In this article, pH-responsive near-infrared emitting conjugated polymer nanoparticles (CPNs) are prepared, characterized, and their stabilities are investigated under various conditions. These nanoparticles have capacity to be loaded with water insoluble, anticancer drug, camptothecin (CPT), with around 10% drug loading efficiency. The in vitro release studies demonstrate that the release of CPTs from CPNs is pHdependent such that significantly faster drug release at mildly acidic pH of 5.0 compared with physiological pH 7.4 is observed. Time and dose-dependent in vitro cytotoxicity tests of blank and CPT-loaded nanoparticles are performed by realtime cell electronic sensing (RT-CES) assay with hepatocellular carcinoma cells (Huh7). The results indicate that CPNs can be effectively utilized as vehicles for pH-triggered release of anticancer drugs.
Magnetic fields with different frequency and intensity parameters exhibit a wide range of effects on different biological models. Extremely low frequency magnetic field (ELF MF) exposure is known to augment or even initiate neuronal differentiation in several in vitro and in vivo models. This effect holds potential for clinical translation into treatment of neurodegenerative conditions such as autism, Parkinson's disease and dementia by promoting neurogenesis, non-invasively. However, the lack of information on underlying mechanisms hinders further investigation into this phenomenon. Here, we examine involvement of glutamatergic Ca 2+ channel, N-methyl-d-aspartate (NMDA) receptors in the process of human neuronal differentiation under ELF MF exposure. We show that human neural progenitor cells (hNPCs) differentiate more efficiently under ELF MF exposure in vitro, as demonstrated by the abundance of neuronal markers. Furthermore, they exhibit higher intracellular Ca 2+ levels as evidenced by c-fos expression and more elongated mature neurites. We were able to neutralize these effects by blocking NMDA receptors with memantine. As a result, we hypothesize that the effects of ELF MF exposure on neuronal differentiation originate from the effects on NMDA receptors, which sequentially triggers Ca 2+-dependent cascades that lead to differentiation. Our findings identify NMDA receptors as a new key player in this field that will aid further research in the pursuit of effect mechanisms of ELF MFs. Keywords Extremely low frequency magnetic field (ELF MF) • Neuronal differentiation • N-methyl-d-aspartate (NMDA) receptor • Human neural progenitor cells (hNPCs) Abbreviations ADAM10 A disintegrin and metalloproteinase AMPA Alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid CREB Cyclic AMP-responsive element-binding protein DAPI 4-6-diamidino-2-phenylindole-dihydrochloride EGFR Epidermal growth factor receptor ELF MF Extremely low frequency magnetic field hNPCs Human neural progenitor cells mT MilliTesla NMDA N-methyl-d-aspartate
This review provides a comprehensive compendium of commonly used biomaterials as well as the different fabrication techniques employed for the design of 3D neural tissue models.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.