This study aimed to evaluate the effect of silver nanoparticles (AgNPs) alone or in combination with calcium hydroxide (Ca(OH)2) on the proliferation, viability, attachment, migration, and osteogenic differentiation of human mesenchymal stem cells (hMSCs). Different concentrations of AgNPs alone or mixed with Ca(OH)2 were prepared. Cell proliferation was measured using AlamarBlue, and hMSCs attachment to dentin disks was evaluated using scanning electron microscopy. Live–dead imaging was performed to assess apoptosis. Wound healing ability was determined using the scratch-migration assay. To evaluate osteogenic differentiation, the expression of Runt-related transcription factor (RUNX2), Transforming growth factor beta-1 (TGF-b1), Alkaline Phosphatase (ALP), and Osteocalcin (OCN) were measured using real-time reverse transcriptase polymerase chain reaction. ALP staining and activity were also performed as indicators of osteogenic differentiation. AgNPs alone seemed to favor cell attachment. Lower concentrations of AgNPs enhanced cell proliferation. AgNP groups showed markedly less apoptosis. None of the medicaments had adverse effects on wound closure. The expression of TGF-b1 was significantly upregulated in all groups, and OCN was highly expressed in the AgNP groups. AgNPs 0.06% showed the most enhanced ALP gene expression levels, activity, and marked cytochemical staining. In conclusion, AgNPs positively affect hMSCs, making them a potential biomaterial for various clinical applications.
This study aimed to compare the effect of thickness and background on the color changes of CAD/ CAM materials (zirconia-reinforced lithium silicate (Suprinity), lithium disilicate (IPS E-max CAD), and hybrid ceramic (Enamic)). Twelve specimens for each thickness (0.4 mm, 0.5 mm, 1 mm, 1.5 mm, and 2 mm) were obtained by sectioning three CAD/CAM materials. A spectrophotometer was used to measure the color change of the specimens against different backgrounds (A2 enamel, A4 dentin, gray, and metal) using the CIELab color space system. Analysis of variance, sample t-test, and posthoc multiple comparison tests were used to evaluate and compare the color changes for different material types, thicknesses, and background colors, with a significance level of P0.05. The result demonstrated that material type, the thickness of the material, and the color of the background had a significant effect on ΔE (P=0.000). Regardless of the material thickness, Suprinity had the highest mean ΔE values (5.4±1.9), and E-max had the lowest mean ΔE values (1.1±0.6). At 2 mm thickness, Enamic presented the lowest ΔE values (0.5±0.5) against the A2 background. The 0.5 and 1 mm specimens corresponded to the highest ΔE values, whereas the 1.5 and 2 mm thickness specimens predominantly gave the lowest ΔE values. All the independent factors, including the type and thickness of the material, and color of the background, contributed significantly to ΔE of the tested materials (P0.05). The study confirms the high masking ability of the lithium disilicate (Emax) material. In clinical situations where the color of the core needs to be masked, it is recommended to use lithium disilicate (E-max).
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