Antibiofilm Efficacy of Silver Nanoparticles Alone or Mixed with Calcium Hydroxide as Intracanal Medicaments: An Ex-Vivo Analysis

AlGazlan, AlMaha S.; Auda, Sayed H.; Balto, Hanan; Alsalleeh, Fahd

doi:10.1016/j.joen.2022.07.008

Cited by 10 publications

(16 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The suspension of silver nanoparticles proved to be the most efficient medium for delivering calcium hydroxide as an intracanal medicament against Candida albicans [ 55 ]. The efficacy of the addition AgNPs in a Ca(OH) 2 mixture showed promising antibacterial properties against a wide range of microorganisms [ 24 , 39 , 46 ]. Their defense mechanism against bacteria was elucidated by the antibacterial activity of the AgNPs; their particles consistently emit silver ions, which can be employed as a tactic for eradicating microorganisms [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

“…Silver nanoparticles (AgNPs) demonstrate bactericidal properties by breaking the cellular membrane, inhibiting metabolic enzymes, and generating reactive oxygen species (ROS) [ 45 ]. Previous research reported the potential antibacterial efficacy of AgNPs when combined with Ca(OH) 2 [ 33 , 39 , 46 ]. Therefore, this study aimed to assess the antifungal activity of a combination of AgNPs and Ca(OH) 2 against C. albicans .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Effectiveness of Silver Nanoparticles Mixed with Calcium Hydroxide against Candida albicans: An Ex Vivo Analysis

Alghofaily,

Alfraih,

Alsaud

et al. 2024

Microorganisms

Self Cite

View full text Add to dashboard Cite

Introduction: The purpose of this study was to assess the antifungal activity of silver nanoparticles (AgNPs) in combination with calcium hydroxide (Ca(OH)2) against Candida albicans (C. albicans). Methods: AgNPs was mixed with pure Ca(OH)2 powder in an aqueous base. A standard suspension (1 × 108 bacterial cells/mL) of C. albicans was prepared in a 96-well plate and incubated on shaker at 37 °C in 100% humidity to allow fungal biofilm formation in infected dentin slices (n = 98). The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of AgNPs alone or with Ca(OH)2 were determined. The samples were separately placed in 24-well tissue culture plates and divided into three experimental groups (0.03, 0.04, and 0.06) and three control groups; negative (saline) and positive chlorhexidine gel and Ca(OH)2. Quantitative measurements of fungal activity by XTT colorimetric assay and qualitative measurements using confocal laser microscopy and scanning electron microscopy were performed. Results: The cell viability of C. albicans in the experimental groups was significantly reduced compared to the negative control group. The combination of (AgNPs (0.04%) and Ca(OH)2) was the most potent against C. albicans. Conclusions: The findings demonstrated that combining silver nanoparticles with Ca(OH)2 was more effective against C. albicans biofilm compared to Ca(OH)2 alone, suggesting a combing effect.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Effectiveness of Silver Nanoparticles Mixed with Calcium Hydroxide against Candida albicans: An Ex Vivo Analysis

Alghofaily,

Alfraih,

Alsaud

et al. 2024

Microorganisms

Self Cite

View full text Add to dashboard Cite

show abstract

“…Enterococcus faecalis , A. viscous and S. mutans are the typical bacterial species of primary and/or secondary endodontic infections were selected [ 26 ]. To facilitate bacterial penetration into the dentine tubules, a centrifuge was used with parameters set at 1400 g , 2000 g , 3600 g and 5600 g for two cycles of 5 min each [ 27 , 28 ]. A total of 120 sterile root-canal models were randomly placed in 10 ml Eppendorf tubes containing 2 ml of each type of bacteria to combine into a 6 ml suspension.…”

Section: Methodsmentioning

confidence: 99%

Thermosensitive hydrogel with programmed dual-octenidine release combating biofilm for the treatment of apical periodontitis

Cheng,

Huangfu,

Zhao

et al. 2024

Regenerative Biomaterials

View full text Add to dashboard Cite

The utilization of intracanal medicaments is an indispensable procedure in root canal treatment（RCT）. However, the conventional intracanal medicaments still need improvement regarding antimicrobial efficacy and ease of clinical operation. To address the above issues, OCT/PECT@OCT+ALK composite hydrogel characterized by programming sequential release of dual antimicrobial agents has been proposed. Thanks to the self-assemble ability of amphiphilic copolymer poly(ε-caprolactone-co-1,4,8-trioxa [4.6]spiro -9-undecanone)-poly(ethylene glycol)-poly(ε-caprolactone-co-1,4,8-trioxa [4.6]spiro-9- undecanone) (PECT), dual hydrophilic and hydrophobic antimicrobial agents could be easily encapsulated in the hydrogel system, and tailored for sequential drug release for a better antibiofilm effect. The hydrophilic octenidine (Octenidine dihydrochloride, OCT-HCl) is encapsulated in the hydrophilic part of hydrogel for instantaneous elevating the drug concentration through bursting release, and the hydrophobic octenidine(Octenidine, OCT) is further loaded into the PECT nanoparticles to achieve a slower and sustained release profile. Additionally, calcium hydroxide (Ca(OH)2) was incorporated into the system and evenly dispersed among PECT nanoparticles to create an alkaline (ALK) environment, synergistically enhancing the antibiofilm effect with higher efficiency and prolonged duration. The antibiofilm effect has been demonstrated in root-canal models and apical periodontitis rats, exhibiting superior performance compared to clinically used Ca(OH)2 paste. This study demonstrates that OCT/PECT@OCT+ALK composite thermosensitive hydrogel is a potential intracanal medicament with excellent antibiofilm effect and clinical operability.

show abstract

“…Growth factors, such as transforming growth factor-beta (TGF-β), bone morphogenetic proteins (BMPs), and vascular endothelial growth factor (VEGF), have already been reported to stimulate cell proliferation and differentiation, angiogenesis, and tissue regeneration of the dental pulp when incorporated into hydrogels [ 29 , 46 , 47 , 48 ]. Additionally, bioactive endodontic hydrogels may include antimicrobial agents, such as chlorhexidine or silver nanoparticles, to avoid bacterial infection and reduce the risk of treatment failure [ 39 , 49 , 50 , 51 , 52 , 53 , 54 ]. Furthermore, bioactive endodontic hydrogels can be clinically administered using minimally invasive techniques, such as syringe injection, allowing for precise placement within the endodontic space.…”

Section: Bioactive Endodontic Hydrogelmentioning

confidence: 99%

Bioactive Endodontic Hydrogels: From Parameters to Personalized Medicine

Leveque,

Bekhouche,

Farges

et al. 2023

IJMS

View full text Add to dashboard Cite

Regenerative endodontic procedures (REPs) aim at recreating dental pulp tissue using biomaterials such as hydrogels. Their bioactivity is mostly related to the nature of biomolecules or chemical compounds that compose the endodontic hydrogel. However, many other parameters, such as hydrogel concentration, bioactive molecules solubility, and apex size, were reported to influence the reciprocal host–biomaterial relationship and hydrogel behavior. The lack of knowledge regarding these various parameters, which should be considered, leads to the inability to predict the clinical outcome and suggests that the biological activity of endodontic hydrogel is impossible to anticipate and could hinder the bench-to-bedside transition. We describe, in this review, that most of these parameters could be identified, described, and studied. A second part of the review lists some challenges and perspectives, including development of future mathematical models that are able to explain, and eventually predict, the bioactivity of endodontic hydrogel used in a clinical setting.

show abstract

Antibiofilm Efficacy of Silver Nanoparticles Alone or Mixed with Calcium Hydroxide as Intracanal Medicaments: An Ex-Vivo Analysis

Cited by 10 publications

References 31 publications

The Effectiveness of Silver Nanoparticles Mixed with Calcium Hydroxide against Candida albicans: An Ex Vivo Analysis

The Effectiveness of Silver Nanoparticles Mixed with Calcium Hydroxide against Candida albicans: An Ex Vivo Analysis

Thermosensitive hydrogel with programmed dual-octenidine release combating biofilm for the treatment of apical periodontitis

Bioactive Endodontic Hydrogels: From Parameters to Personalized Medicine

Contact Info

Product

Resources

About