No abstract

BackgroundAging is typically accompanied by memory decline and changes in hippocampal function. Among these changes is a decline in the activity of the dentate gyrus (DG) during behavior. Lasting memory, however, is thought to also require recapitulation of recent memory traces during subsequent rest – a phenomenon, termed memory trace reactivation, which is compromised in hippocampal CA1 with progressive age. This process has yet to be assessed in the aged DG, despite its prominent role in age-related memory impairment. Using zif268 transcription to measure granule cell recruitment, DG activity in adult and aged animals was assessed both during spatial exploration and as animals remained at rest in the home cage in order to detect potential memory-related replay.ResultsConsistent with the observation of memory trace reactivation in DG, the probability that an individual granule cell transcribes zif268 during rest in the animal’s home cage is increased by recent experience in a novel environment. Surprisingly, a comparable increase was observed in the probability of granule cells in the aged DG expressing zif268 during rest. Moreover, no significant age-related difference was observed in the number of granule cells expressing zif268 during rest. Thus, the number and pattern of granule cell expression of zif268 during rest is preserved in aged animals, despite a significant decline in exploration-related zif268 expression.ConclusionsThese data lead to the hypothesis that the input the aged DG receives from backprojections from CA3 (the region widely hypothesized to mediate reactivation) remains functionally intact despite loss of innervation from the perforant path.

Following training to match 2-and 8-sec durations of feederlight to red and green comparisons with a 0-sec baseline delay, pigeons were allowed to choose to take a memory test or to escape the memory test. The effects of sample omission, increases in retention interval, and variation in trial spacing on selection of the escape option and accuracy were studied. During initial testing, escaping the test did not increase as the task became more difficult, and there was no difference in accuracy between chosen and forced memory tests. However, with extended training, accuracy for chosen tests was significantly greater than for forced tests. In addition, two pigeons exhibited higher accuracy on chosen tests than on forced tests at the short retention interval and greater escape rates at the long retention interval. These results have not been obtained in previous studies with pigeons when the choice to take the test or to escape the test is given before test stimuli are presented. It appears that task-specific methodological factors may determine whether a particular species will exhibit the two behavioral effects that were initially proposed as potentially indicative of metacognition.

Pigeons were trained in a within-subjects design to discriminate empty intervals (bound by two 1-sec visual markers) and filled intervals (a continuous visual signal). The intervals were signaled by different visual stimuli and they required responses to different sets of comparison stimuli. In Experiment 1, empty intervals were judged longer than filled intervals. The difference between the point of subjective equality (PSE) for the empty intervals and the PSE for filled intervals increased as the magnitude of the anchor-duration pairs increased. Although there was more pecking during filled intervals than during empty intervals, there was no significant correlation between pecking during filled intervals and the value of the PSE. In Experiment 2, empty intervals continued to be judged longer than filled intervals, even when pigeons were required to refrain from pecking during filled intervals. Keypecking per se does not appear to play an important role in the empty-filled timing difference.

and her Bachelor of Science in Mechanical Engineering from the George Washington University. Her research aims to acquire new fundamental understanding of phase-change processes. She is passionate about research, education, and mentoring. She is also committed to creating a more inclusive engineering culture.