Paleomobility has been a key element in the study of the expansion of ancient states and empires, including the Tiwanaku polity of the South Central Andes (AD 500-1000). We present radiogenic strontium and oxygen isotope data from human burials from three cemeteries in the Tiwanaku-affiliated Middle Horizon archaeological site complex of Rio Muerto in the Moquegua Valley of southern Peru. At Rio Muerto, archaeological human enamel and bone values range from (87) Sr/(86) Sr = 0.70657-0.72018, with a mean of (87) Sr/(86) Sr = 0.70804 ± 0.00207 (1σ, n = 55). For the subset of samples analyzed for oxygen isotope values (n = 48), the data ranges from δ(18) Ocarbonate(VSMOW)  = +18.1 to +27.0‰. When contextualized with other lines of archaeological evidence, we interpret these data as evidence for an archaeological population in which the majority of individuals had "local" origins, and were likely second-generation, or more, immigrants from the Tiwanaku heartland in the altiplano. Based on detailed life history data, we argue a smaller number of individuals came at different ages from various regions within the Tiwanaku polity. We consider whether these individuals with isotopic values consistent with "nonlocal" geographic origins could represent first-generation migrants, marriage exchange partners, or occupationally mobile herders, traders or other travelers. By combining isotopic life history studies with mortuary treatment data, we use a person-centered migration history approach to state integration and expansion. Isotopic analyses of paleomobility at the Rio Muerto site complex contribute to the role of diversity in ancient states by demonstrating the range of geographic origins rather than simply colonists from the Lake Titicaca Basin.

These data indicate relatively high C4 plant consumption among the Tiwanaku colonies, and support paleobotanical and archaeological evidence that maize (Zea mays) was the staple crop. Dietary values are similar overall between the sexes, but significantly higher δ(13) Ccollagen values in males is consistent with a model of gendered norms of consumption similar to that of the later Inca (AD 1438-1533), where males consumed more maize than females, often in the form of beer (chicha). Results provide new insights on social dynamics within the Tiwanaku colonies and suggest the increased importance maize consumption for males during the Tiwanaku expansion.

Guinea pigs (Cavia spp.) have a long association with humans. From as early as 10,000 years ago they were a wild food source. Later, domesticated Cavia porcellus were dispersed well beyond their native range through pre-Columbian exchange networks and, more recently, widely across the globe. Here we present 46 complete mitogenomes of archaeological guinea pigs from sites in Peru, Bolivia, Colombia, the Caribbean, Belgium and the United States to elucidate their evolutionary history, origins and paths of dispersal. our results indicate an independent centre of domestication of Cavia in the eastern colombian Highlands. We identify a peruvian origin for the initial introduction of domesticated guinea pigs (Cavia porcellus) beyond South America into the caribbean. We also demonstrate that peru was the probable source of the earliest known guinea pigs transported, as part of the exotic pet trade, to both Europe and the southeastern United States. Finally, we identify a modern reintroduction of guinea pigs to Puerto Rico, where local inhabitants use them for food. This research demonstrates that the natural and cultural history of guinea pigs is more complex than previously known and has implications for other studies regarding regional to global-scale studies of mammal domestication, translocation, and distribution. The use of ancient DNA (aDNA) in studies of animal domestication and subsequent translocation has radically improved our ability to identify spatially, temporally, and culturally variable processes of domestication and the diversity of social networks behind domestic species distribution (e.g. 1,2). Increasingly, aDNA studies are revising previous assumptions of geographically conscripted animal domestication and dispersal events to reveal multiple centers, timings, and processes of domestication of the world's most prominent domestic animals (e.g. pigs, chickens, cattle, dogs 3-6). Because domestic animals are exemplar proxies for investigating past human migration and interaction, understanding long-term, diachronic patterns of when and where species domestication and

The analysis of 87 Sr/ 86 Sr has become a robust tool for identifying non-local individuals at archeological sites. The 87 Sr/ 86 Sr in human bioapatite reflects the geological signature of food and water consumed during tissue development. Modeling relationships between 87 Sr/ 86 Sr in human environments, food webs, and archeological human tissues is critical for moving from identifying non-locals to determining their likely provenience. In the Andes, obstacles to sample geolocation include overlapping 87 Sr/ 86 Sr of distant geographies and a poor understanding of mixed strontium sources in food and drink. Here, water is investigated as a proxy for bioavailable strontium in archeological human skeletal and dental tissues. This study develops a water 87 Sr/ 86 Sr isoscape from 262 samples (220 new and 42 published samples), testing the model with published archeological human skeletal 87 Sr/ 86 Sr trimmed of probable non-locals. Water 87 Sr/ 86 Sr and prediction error between the predicted and measured 87 Sr/ 86 Sr for the archeological test set are compared by elevation, underlying geology, and watershed size. Across the Peruvian Andes, water 87 Sr/ 86 Sr ranges from 0.7049 to 0.7227 (M = 0.7081, SD = 0.0027). Water 87 Sr/ 86 Sr is higher in the highlands, in areas overlying older bedrock, and in larger watersheds, characteristics which are geographically correlated. Spatial outliers identified are from canals, wells, and one stream, suggesting those sources could show non-representative 87 Sr/ 86 Sr. The best-fit water 87 Sr/ 86 Sr isoscape achieves prediction errors for archeological samples ranging from 0.0017-0.0031 (M = 0.0012, n = 493). The water isoscape explains only 7.0% of the variation in archeological skeletal 87 Sr/ 86 Sr (R 2 = 0.07), but 90.0% of archeological skeleton 87 Sr/ 86 Sr fall within the site isoscape prediction ± site prediction standard error. Due to lower sampling density and higher geological variability in the highlands, the water 87 Sr/ 86 Sr isoscape is more useful for ruling out geographic origins for lowland

This Article contains errors. The Acknowledgements section in this Article is incomplete. "We would like to thank Matthew Smith for providing the Green Castle, Jamaica specimen; Kitty Emery and the Environmental Archaeology Program Laboratory at the Florida Museum of Natural History for facilitating access to guinea pig specimens from Finca Valencia, Puerto Rico (Catalog #0547); William Keegan and Corinne Hofman for analysis of specimens from Giraudy, Saint Lucia; Daniel S. Sandweiss for permission to analyze the Lo Demás specimens housed at the Florida Museum of Natural History and site information; Ana Maria Boada for providing samples from El Venado, Colombia; and Martha Zierden (on behalf of Charleston Museum) and Elizabeth Reitz for providing the Charleston sample, site information and comments on the manuscript. Kuelap samples were selected and exported legally according to the Ministry of Culture of Peru Resolución Viceministerial no. 095-2015-VMPCIC-MC (August 2015). We thank Susan Duser for assistance in creating Figs. 1A and 2. We acknowledge Peter Stahl for his review of the manuscript prior to publication.