Aim of this study was to analyze phytochemical constituents and antibacterial activity of Urtica dioica leave extracts against isolated foodborne pathogens from different food samples. The phytochemical analysis showed the presence of carbohydrates, alkaloids, saponins, tannins and protein. Total 75 different isolates of bacteria were isolated from different food samples. Out of 75twenty-four were found to be positive for Klebsiella, twenty-six for Staphylococcus spp. and twenty-five for E. coli. Maximum zone of inhibition was recorded by ethyl acetate extract against E. coli with 2.36±0.26 mm diameter followed by Klebsiella (2.28±0.16 mm) Staphylococcus spp. (1.96±0.28 mm) respectively. Methanol extract showed maximum inhibitory zone of 1.91±0.26 mm against Klebsiella followed by 1.82±0.33 mm zone against Staphylococcus spp. and 1.5±0.17 mm inhibitory zone against E. coli. Aqueous extract not showed inhibition against any isolated bacteria.
Urtica dioica (Stinging nettle) belonging to the family Urticaceae is a medicinal plantfound in the Himalayas region from Kashmir to Kumaon region (Uttarakhand.). In Himalayan region, this plant is used for the daily purpose as food as well as to serve animals. It contains various phytochemicals like phenolic acid, coumarins, flavanols, bioflavonoids, Flavan-3-ols etc which is used to cure many diseases like arthritis, constipation, Pulmonary, Liver, Intestine, Kidney, Diabetes, Fungal infections, anti-inflammatory, internal injury and wounds. It also has hepatoprotective activity, anti‑hyperlipidemic activity, diuretic activity, antimicrobial activity, anticancer activity. Researchers have reported about 30 species with 47 genera and 1,300 species worldwide used to treat different diseases. Medicinal plants have proved to be the “backbone” of traditional medical system which signifies that developing countries depend upon medicinal plants for therapeutic use.
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