Understanding citrus tree root development and dynamics are critical in determining crop best nutrient management practices. The role of calcium (Ca) and magnesium (Mg), manganese (Mn), Zinc (Zn), and boron (B) on huanglongbing (HLB) affected citrus trees' root growth and lifespan in Florida is not fully documented. Thus, the objective of this study was to determine the impact of foliar and ground-applied essential nutrients on seasonal fine root length density (FRLD; diameter (d) < 2 mm) and coarse roots (d > 2 mm), FRLD dynamics, root survival probability (lifespan), and root-zone soil pH of HLB-affected sweet orange trees. Results indicated that Ca treated trees budded on Cleopatra (Cleo) and Ca and Mg combined treatments on Swingle (Swc) rootstocks significantly increased seasonal FRLD of fine (< 2 mm) and coarse roots. The highest median root lifespan of Ca treated trees was 325 and 339 days for trees budded on Cleo and Swc rootstocks, respectively. In the second study, the coarse roots showed a significantly higher reaction to the nutrition applied than the fine roots. Meanwhile, the 2× (1× foliar and 1× ground-applied) treated trees showed a significantly higher median root lifespan compared to the other treatments. Thus, the current study unwraps future studies highlighting the combined soil and/or foliar application of the above nutrients to stimulate FRLD and improve root lifespan on HLB-affected sweet oranges with emphasis on root-zone soil pH.Plants 2020, 9, 483 2 of 20 to 2.02 cmcm -3 for trifoliate orange (Poncirus trifoliata). Although the citrus root system is estimated to account for more than 65% of above-ground dry mass [12,13] the root system of HLB-affected citrus trees is usually poorly developed and new root growth can also be inhibited [14]. Studies indicated that 30%-50% of roots of HLB-affected citrus trees are impaired before canopy symptoms appear and 70%-80% of root loss could be prevalent as citrus deprived of intensive cultural management to pacify abiotic and biotic stress [10,15]. The optimum distribution of the root system is mainly restricted by water and nutrients available in soil profiles [16,17]. However, it has been reported that HLB develops an imbalance of nutrient concentrations, which cause nutrient toxicity for some nutrients, including Cu or deficiency for others such as Ca, Mg, Mn, and Zn [18]. Therefore, nutrient supply is an imperative aspect of disease control because nutrients influence plant resistance, pathogen vigor, growth, and associated factors [19,20].Ground-applied fertilizers are subject to unfavorable soil processes such as precipitation as forms that are unavailable to plants, leaching, and runoff [21,22]. Therefore, split ground and foliar-applied fertilizer improve nutrient use efficiency and reduce both nutrient leaching and application cost [23][24][25]. Moreover, HLB-affected trees are limited in soil nutrient uptake because of the associated decline in FRLD [12,16,26]. The role of adding essential nutrients in improving citrus growth in general a...

The decrease in the rate of inflow and outflow of water—and thereby the uptake of plant nutrients as the result of Huanglongbing (HLB or citrus greening)—leads to a decline in overall tree growth and the development of nutrient deficiencies in HLB-affected citrus trees. This study was conducted at the University of Florida, Southwest Florida Research and Education Center (SWFREC) near Immokalee, FL from January 2017 through December 2019. The objective of the study was to determine the effect of rootstocks, nutrient type, rate, and frequency of applications on leaf area index (LAI), water relations (stomatal conductance [gs], stem water potential [Ψw], and sap flow), soil nutrient accumulation, and dynamics under HLB-affected citrus trees. The experiment was arranged in a split-split plot design that consisted of two types of rootstocks, three nitrogen (N) rates, three soil-applied secondary macronutrients, and an untreated control replicated four times. LAI significantly increased in response to the secondary macronutrients compared with uncontrolled trees. A significantly greater gs, and thus a decline in Ψw, was a manifestation of higher sap flow per unit LA (leaf area) and moisture stress for trees budded on Swingle (Swc) than Cleopatra (Cleo) rootstocks, respectively. The hourly sap flow showed significantly less water consumption per unit LA for trees that received a full dose of Ca or Mg nutrition than Ca + Mg treated and untreated control trees. The soil nutrient concentrations were consistently higher in the topmost soil depth (0–15 cm) than the two lower soil depths (15–30 cm, 30–45 cm). Mobile nutrients: soil nitrate–nitrogen (NO3-N) and Mg2+ Mg2+, Mn2+, Zn2+, and B leached to the lower soil (15–30 cm) depth during the summer season. However, the multiple split applications of N as Best Management Practices (BMPs) and optimum irrigation scheduling based on reference evapotranspiration (ETo) maintained soil available N (ammonium nitrogen [NH4-N] and NO3-N) below 4.0 mg kg−1, which was a magnitude 2.0–4.0× less than the conventional N applications. Soil NH4-N and NO3-N leached to the two lower soil depths during the rainy summer season only when trees received the highest N rate (280 kg ha−1), suggesting a lower citrus N requirement. Therefore, 224 kg ha−1 N coupled with a full Ca or Mg dose could be the recommended rate for HLB-affected citrus trees.

The fate of foliar and ground-applied essential nutrients is the least studied topic under citrus greening or Huanglongbing (HLB)-affected citrus, which is inherently suffering from severe root decline because of HLB-associated problems. The objective of this study was to evaluate if ground-applied coupled with foliar spray of essential nutrients can reverse the decline in tree growth and understand the fate of the nutrients in the soil-root-tree interfaces. The treatments were arranged in a split-split plot design in which nitrogen (N) was ground-applied in 20 splits biweekly and Mn, Zn, and B were foliar and /or ground-applied in three splits following the spring, summer, and late summer flush seasons. Soil nutrients in three depths (0–15, 15–30, and 30–45 cm), root, and leaf nutrient concentrations of the essential nutrients, leaf area index (LAI), and tree canopy volume (TCV) data were studied twice (spring and summer) for two years. A significantly higher soil NH4-N and NO3-N concentrations were detected in the topsoil depth than the two lower soil depths (15–30 and 30–45 cm) indicating lesser nutrient leaching as trees received moderate (224 kg ha−1) N rate. Except for soil zinc (Zn) concentration, all the nutrient concentrations were significantly higher in the topsoil (0–15 cm), compared with two lower soil depths indicating that Zn was intricate by changes in soil environmental conditions, root acquisition, and/or leaching to lower soil depth. Leaf N concentration significantly increased over time following seasonal environmental fluctuations, tree growth, and development. Thus, leaf N concentration remained above the optimum nutrient range implying lower N requirement under irrigation scheduling with SmartIrrigation, an App used to determine the daily irrigation duration to meet tree water requirement and split fertigation techniques. Root Manganese (Mn) and Zn concentrations were significantly higher in the root tissues of the treated than the control trees and translocated to the leaves accordingly. Meanwhile, a significantly higher LAI for trees budded on Swingle (Swc) rootstock however, larger TCV for trees budded on Volkameriana (Volk) rootstocks. The trees had significantly larger TCV when the trees received a moderate N rate during early study years and under foliar 9 kg ha−1 coupled with the ground 9 kg ha−1 Mn and Zn treatments during the late study years. Therefore, split ground application of 224 kg ha−1 of N, foliar applied 9 kg ha−1 coupled with ground-applied 9 kg ha−1 Mn and Zn were the suggested rates to sustain the essential leaf nutrient concentration within the optimum ranges and improve the deterioration of vegetative growth associated with HLB-induced problems of citrus trees.

Nitrogen and micronutrients have a key role in many citrus plant enzyme reactions. Although enough micronutrients may be present in the soil, deficiency can develop due to soil depletion or the formation of insoluble compounds. The objectives of this study were to (1) determine the adsorption, distribution, and availability of Zn in a sandy soil; (2) compare the effectiveness of foliar and soil application methods of Zn on Huanglongbing [HLB] affected trees; (3) compare foliar application rates of Zn for HLB-affected trees; (4) determine the effect of N rates on yield, soil inorganic N distribution patterns, and tree growth parameters. Tree rows were supplied with three N rates of 168, 224 and 280 kg·N·ha−1 and Zn at single and double recommended rates (recommended rate = 5.6 kg·Zn·ha−1) using foliar and soil application methods, in a split-plot experimental design. The results show that Zn concentration in the 0–15 cm soil depth was three times higher than the 30–45 and 45–60 cm soil depths during the study. An adsorption study revealed high Zn (KD = 6.5) sorption coefficients at 0–15 cm soil depth, while 30–45 and 45–60 cm depths showed little sorption. Leaf Zn concentration for foliar spray was two times higher than the soil application method. A nitrogen level of 224 kg N ha−1 improved canopy volume when compared to other N levels at the expense of reduced fruit weight. Foliar Zn application at 5.6 or 11.2 kg ha−1 and N rate at 224 kg ha−1 appear to be adequate for improving the performance of HLB-affected citrus trees.

 Split application of ground applied essential nutrients increase the availability of nutrients and decrease higher nutrient requirements. Split application of essential nutrients improves the availability and uptake of nutrients for adversely affected HLB-induced root density citrus trees, thus decrease leaching and reduce downstream ecosystems. The availability of essential nutrients increases vegetative growth. Vegetative growth improves fruit yield and quality.

In the western Sahel, leaves of Roselle (Hibiscus sabdariffa) have considerable economic importance because of their nutritional and medical uses. These plant organs are used to supplement nutrients provided by cereals such as millet and sorghum. However, there is a lack of information on the nutrient composition of these plant organs of Roselle at different growth stages. Therefore, the experiment was carried out under rainfall conditions during the 2006 rainy season (from July to September) at the experimental station of the Agrhymet Regional Centre in Niamey (Niger). The content of the micronutrients Fe, Mn, Cu and Zn in leaves of three ecotypes of Roselle (A3, A7 and A9) at three growth stages, vegetative (stage I), flowering (stage II), and mature (stage III) was determined. The experimental design was a randomized complete block with four replicates and one variable (ecotype). Results indicated that at stage I, ecotype A3 had higher Fe content in leaves. In addition, A3 had also the highest Zn content in leaves at stage I. For all three ecotypes, Fe and Zn content in the leaves decreased significantly (p<0.05) from stage I to stage II, then remained constant until stage III. For Fe, the decrease between stage I and II was 37% for A3 and 50%, respectively for A7 and A9. The corresponding decrease of Zn content was 30% for A7 and 50%, respectively, for A3 and A9. The Mn content in the leaves of Roselle was similar for the three ecotypes at stage I, thereafter increased continuously during plant growth. From stage I to II, the increase was about 90%, 70% and 50%, respectively for A9, A7 and A3. From stage II to III, the increase in Mn content in the leaves was significantly (p<0.05) higher for A3 and A7, respectively 180% and 80%. At stages I and II, the highest Cu content was recorded for A3 and the lowest one for A7. During the whole cycle of plant growth, the Cu content in the leaves was relatively constant for A9. In contrast, Cu content in the leaves decreased for the remaining ecotypes. Therefore the vegetative stage corresponding to 25 days after sowing is the recommended optimal harvest time of Roselle to maximise on the nutrients.

Nutrients are vital for plant growth, development, and aid in disease control because nutrients affect host plant and pathogen interactions. Once a citrus tree is infected with the phloem-limited, Gram-negative bacteria, Candidatus Liberibacter asiaticus (CLas), huanglongbing (HLB; citrus greening), it would fall under threat of survival as the disease has no known control mechanism discovered thus far. The objective of this study was to determine if split soil applications of essential nutrients improve the availability and accumulation, reduce leaching of these nutrients beyond the root zone, and promote root growth and water dynamics of HLB-affected citrus trees in the soil–plant–atmosphere continuum. Split soil applications of three N rates (168, 224, and 280 kg ha−1 year−1) were the main blocks. Micronutrients were randomly applied to the sub-blocks assigned in a split-split plot design, applied in three splits annually. The micronutrients were applied to foliage and soil as follows: foliar only 1× (1×), foliar 1× and soil-applied 1× (2×), and foliar 1× and soil-applied 2× (3×)× (1× = 9 kg ha−1 year−1 of Mn and Zn to each foliar and soil along with 2.2 kg ha−1 year−1 of B). Significant soil NH4-N and NO3-N, Zn, and Mn were retained within the active soil root zone (0–30 cm). Higher soil acidity was detected when trees received the highest micronutrient rate in the upper soil layers (0–15 cm) as compared with the middle (15–30 cm) and the lowest (30–45 cm) soil layers. Fine root length density (FRLD) was significantly lower at the highest micronutrient rates, manifesting root growth negatively associated with high Mn and low soil pH. Invariably, the water dynamics: stem water potential (ψstem), stomata conductance (gs), and sap flow were also negatively affected when trees received foliar 1× and soil 2× (3×) treatment as compared with the other treatments. Split application of nutrients had a significant effect on FRLD growth, retaining soil-applied nutrients within the active root zone, and improved water use efficiency.

Since the first occurrence of Huanglongbing (HLB) in the Florida commercial citrus industry in 2004, fruit yield and yield components of HLB-affected citrus have declined in endemically affected citrus tree groves. Optimal fertilization is thus critical for improving tree performance because nutrients are vital for tree growth and development, and play a significant role in tree disease resistance against various biotic and abiotic stresses. The objective of the current study was to determine whether leaf nutrient concentration, tree growth, yield, and postharvest quality of HLB-affected citrus trees were improved by the split application of nutrients. The four micronutrient application rates were used as fixed factors and the three nitrogen (N) rates were used as random factors for leaf nutrient analyses, tree growth, fruit yield, and postharvest analyses. Significant leaf manganese (Mn) and zinc (Zn) concentrations were detected when trees received foliar and soil-applied micronutrients regardless of the N rates. There was a strong regression analysis of leaf Mn and Zn nutrient concentration and nutrient rates with R2: 0.61 and 0.59, respectively. As a result, a significant leaf area index associated with foliar and soil-applied micronutrient rates had a positive correlation with leaf area index and soil pH with R2: 0.58 and 0.63 during the spring and summer seasons, respectively. Trees that received a moderate (224 kg·ha−1) N rate showed the least fruit decay percentage and total soluble solids (TSS) of 8% more than the lowest (168 kg·ha−1) and highest (280 kg·ha−1) N rates, even though fruit yield variations were barely detected as these micronutrients promoted vegetative growth. Moreover, the TSS to titratable acidity (TA) ratio of foliar and soil-applied micronutrient-treated trees showed 2% and 7% greater values than the foliar-only treated and control trees, respectively. Although micronutrients exacerbated stem-end rind breakdown (SERB), these nutrients significantly improved fruit storage when the fruits were stored for extended periods (8–11 weeks). Thus, moderate N rate, foliar (1×), and soil-applied (1×) micronutrient treatments improved tree growth, fruit postharvest, and fruit storage characteristics.