Resistance phenomena of infectious agents to synthesis drugs and the high cost of these drugs led to medicinal plants as an alternative against infectious disease. The objective of this study is to characterize different classes of secondary metabolites and to test the antibacterial activity of six plants: Bauhinia rufescens Lam, Blepharis linariifolia PERS, Chrozophora brocchiana Vis, Gardenia ternifolia Schum and Thonn, Indigofera astragalina DC, Phyllanthus pentandrus Schumach &Thonn, used in traditional medicine for children treatment. The extracts are obtained by aqueous decoction with the 1/10 model (50 g of each sample in 500 ml of solvent). Phytochemical screening of these six plants was carried out by standards tests, which are reactions of colorations, precipitation and complexation. The agr medium diffusion was used for the sensitivity test of the aqueous extracts of these plants against two strains of bacteria (two isolates of Salmonella typhimurium lettuce and clinical). Phytochemical screening showed the presence of polyphenols, tannins, flavonoids, coumarins, alkaloids, sterols/triterpenes in all tested extracts. All extracts contain saponins except Phyllanthus pentandrus. For free quinones, only Phyllanthus pentandrus and Indigofera astragalina showed a negative test. Among these six plants, five (5) showed an activity on Salmonella typhimurium at 200 mg/mL. C. brocchiana and P. pentandrus showed the best activity on S. typhimurium (lettuce) with 19.5±0.70 mm and 17.5±0.70 mm, respectively. At the concentration of 50 mg/mL, only these two plants are also showed antibacterial activity on S. typhimurium (lettuce). The results obtained indicate that these plants contain some secondary metabolites, some have antimiocrobial activity and constitute a scientific database for the research in the field of phytomedicines. These results will justify the use of these plants in the treatment of some infections.
Objective: The aim of this study is to determine the prevalence and phenotype diversity of Salmonella isolated from poultry offal in Niger. Methodology and Results: A total of 155 poultries offal consisting of gizzard, liver and spleen were analyzed according to ISO 6579: 2002. Based on these different analyzes, high prevalence of Salmonella from 20% to 69% was found. Serotyping showed the predominance of Derby 42.37% followed by S. Hato 15.25%, S. Chester 10.17%, S. Agona 5.08%, S. Suberu and S. Essen 3.39% each, S. Hessarek and S. Kissangani 1.69% each. Isolated Salmonella strains showed low resistance to antibiotics. Conclusion and perspective: Poultry offal for human consumption has high concentration of Salmonella. This is due to poor hygienic practices of poultry sellers. From these facts, awareness and training measures are necessary. Niger authorities must also build modern slaughterhouses and poultry markets in order to reduce the risk infectious proliferation of diseases such as gastroenteritis and food poisoning.
International trade, new agricultural, animal production and food practices have facilitated the spread and transmission of food-borne pathogens; including Salmonella. Salmonella is a ubiquitous bacterium responsible for a variety of diseases in humans, animals. It has a large diversity and contains more than 2579 serotypes. However, very little work describing the diversity, structure and populations dynamics of Salmonella in different hosts is available in Niger. In order to describe the general context of Salmonella diversity in Africa, an analysis of the available literature on this subject was carried out. A total of 131 publications were identified. From those publications, twenty five (25) were eligible. The LOGIT estimation model was used for serotype analysis to standardize the distribution of data and minimize sample sizes. It appears from our analysis that the prevalence of Salmonella is high regardless of the host and host germ. The major serotypes found in humans in Africa are S. Typhimurium, S. Enteritidis and S. Typhi. In poultry, the predominant serotype is S. Hadar on the other side S. Typhimurium predominates in North Africa. Thus, the results of these review analyzes constitute one of the steps in the process of understanding trends in the distribution of Salmonella in Africa. These results are presented as an outline of the implementation of a successful
The emergence of multidrug bacterial resistance poses a great public health problem and requires a constant search for new antibacterial agents. However, Niger’s flora possesses several medicinal plants used in traditional medicine to cure infectious diseases and can be used as sources of bioactive ingredients. This current study was designed to evaluate the antibacterial activity of eight plants used in the traditional pharmacopeia of Niger. The extracts were prepared by maceration using ethanol, methanol, and distilled water. The obtained extracts were screened against Salmonella spp., Shigella spp., and Escherichia coli using the microdilution method coupled with a resazurin-based assay. Phytochemical screening was performed using colorimetry, while the quantification of total polyphenols, total flavonoids, and total tannins was determined by spectrophotometry. Out of the eight plants obtained, five named Cassia italica, Limeum pterocarpum, Phyllanthus pentandrus, Strychnos innocua, and Ximenia americanum exhibited antibacterial activity with MICs ranging from 500 μg/mL to 2000 μg/mL. Phytochemical screening showed the presence of alkaloids, saponosides, tannins, flavonoids, terpenes/sterols, quinones, and polyphenols. The ethanolic and methanolic extracts of X. americana contained important quantities of total polyphenols, with 43.59 ± 0.15 and 41.97 ± 0.02 mg EAG/100 mg of extract, respectively. These extracts showed the highest contents of total tannins at 46.49 g/L and 45.52 g/L, respectively. For total flavonoids, the highest content was obtained with the methanolic extract of P. pentandrus, with 3.12 ± 0.01 mg QE/100 mg of extract. These findings justify the uses of these plants in traditional medicine for the treatment of infectious diseases such as diarrhea and can be used as starting points for the development of phytodrugs against infectious diarrhea.
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