Hantaviruses are members of the family Bunyaviridae and are an emerging cause of disease worldwide with high lethality in the Americas. In Brazil, the diagnosis for hantaviruses is based on immunologic techniques associated with conventional RT-PCR. A novel one-step SYBR Green real-time RT-PCR was developed for the detection and quantitation of Araraquara hantavirus (ARAV) and Rio Mamore hantavirus (RIOMV). The detection limit of assay was 10copies/µL of RNA in vitro transcribed of segment S. The specificity of assay was evaluated by melting curve analysis, which showed that the Araraquara virus amplified product generated a melt peak at 80.83 ± 0.89 °C without generating primer-dimers or non-specific products. The assay was more sensitive than conventional RT-PCR and we detected two samples undetected by conventional RT-PCR. The one-step SYBR Green real-time quantitative RT-PCR is specific, sensible and reproducible, which makes it a powerful tool in both diagnostic applications and general research of ARAV and RIOMV and possibly other Brazilian hantaviruses.
Pemphigus vulgaris (PV) and pemphigus foliaceus (PF) are blistering autoimmune diseases that depend on interaction between genetic and environmental factors. Viral infections, like herpes simplex viruses 1 and 2 (HSV1/2), cytomegalovirus (CMV), Epstein‐Barr virus and dengue virus, could trigger or exacerbate pemphigus. IgM and IgG antibodies against these viruses in serum from PV and PF, their relatives and controls were determined. HSV1/2 expression was evaluated by direct immunofluorescence (DIF) and qPCR in affected or not oral mucosa from PV patients compared with uninjured PF mucosa. IgG anti‐HSV1 was higher in the PV group compared with all groups. IgG anti‐CMV resulted higher in PV group compared with PF patients and PV relatives. HSV1 was confirmed by DIF and qPCR on oral samples from patients with PV. Lack of HSV1 expression in the oral mucosa of patients with PF corroborate that immunosuppressive therapy cannot be the main cause for HSV1 replication in PV disease.
INTRODUCTION: Arenavirus hemorrhagic fever is a severe emerging disease. METHODS: Considering that the levels of antibodies against arenavirus in the Brazilian population are completely unknown, we have standardized an ELISA test for detecting IgG antibodies using a recombinant nucleoprotein from the Junin virus as the antigen. This protein was obtained by inserting the gene of the Junin virus nucleoprotein into the genome of Autographa californica nucleopolyhedrovirus, using the Bac-to-Bac baculovirus expression system. This recombinant baculovirus was used to infect S. frugiperda cells (SF9). RESULTS: The infection resulted in synthesis of high concentrations of recombinant protein. This protein was detected on 12.5% polyacrylamide gel and by means of Western blot. Using the standardized ELISA test, 343 samples from the population of Nova Xavantina were analyzed. We observed that 1.4% of the serum samples (five samples) presented antibody titers against arenavirus. CONCLUSIONS: These results show the population studied may present exposure to arenavirus infection.
SUMMARYHantavirus cardiopulmonary syndrome (HCPS) is an infectious disease caused by hantaviruses of the family Bunyaviridae, and is transmitted by aerosols of excreta of infected rodents. The aim of the present study was to determine antibody levels to hantavirus in the population that lives at frontier of Brazil and Argentina. Participated of the study 405 individuals living in the municipalities of Bandeirante, Santa Helena, Princesa and Tunapolis, state of Santa Catarina, Brazil. IgG antibodies to hantavirus were analyzed in sera by an ELISA that uses a recombinant N protein of Araraquara hantavirus as antigen. The results were also confirmed by immunofluorescent test. Eight individuals showed antibodies to hantavirus (1.97% positivity), with serum titers ranging from 100 to 800. Six seropositives were males, older than 30 years and farmers. Our results reinforce previous data on hantavirus circulation and human infections in the southern border of Brazil with Argentina.
Insecticide resistance has caused difficulties in controlling Aedes aegypti, stimulating the search for natural larvicide compounds. Crotalaria pallida, popularly known as Chique-Chique and for its repellent action, still lacks scientific data regarding its properties. The objective was to verify the larvicidal/ovocidal potential of this plant in A. aegypti. C. pallida leaves were subjected to extraction with solvents: Ethyl Acetate (AcEt), Hexane (Hex) Ethanol (EtOH) and Methanol (MetOH). Larvicidal action was determined with 15 larvae (L2, Rockefeller strain) through contact with each extract (500, 250; 125; 62.5; 31.25 mg/L diluted in dimethyl sulfoxide (DMSO) for 24 hours (triplicate tests)) with subsequent determination of the lethal dose (DL90 and DL50) .The LD50 was used to determine the larvicidal action in the different larval instars for the extracts with greater lethality. at 500 mg/L) for 10 minutes and subsequently transferred to clean water for hatching (Contact Test). Eggs were also hatched in water containing 500 mg/L of the extracts (Hatching test). All results were subjected to statistical analysis, a better larvicidal action was observed, with death of 100% and 88.8%, in the compounds MetOH and EtOH, respectively, at a concentration of 500 mg/L.
Anti-Dsgs in ATL may represent epiphenomena. Anti-Lbr-pep antibodies in pemphigus suggest a previous infection. A differential association of the HLA profile may contribute to the lack of co-association between pemphigus and ATL.
Avaliação do perfil epidemiológico das neoplasias onco-hematológicas de pacientes atendidos pelo instituto de câncer de três lagoas, no período de 2014 a 2018 Evaluation of the epidemiological profile of oncohematological neoplasms of patients carried out by neoplasm institute of três lagoas, 2014-2018
As doenças priônicas (DPr) humanos são um grupo de doenças neurodegenerativas progressivas, incuráveis e fatais causadas por um agente infeccioso proteico (PrP), capaz de propagar a doença alterando a estrutura conformacional de proteínas, as quais sofrem agregação e depósito no tecido neuronal. Devido ao potencial neurodegenerativo desta doença, bem como a um aumento global de casos, o objetivo do nosso artigo é revisar o conhecimento atual sobre DPr humanas e analisar a situação epidemiológica dessas doenças, no Brasil nos últimos 17 anos. Sabemos, que existem diferentes tipos de DPr com diferenças relacionadas a sua forma de transmissão/manifestação, neuropatologia e manifestações clínicas e que os príons consistem em PrPSc a forma patológica agregada da proteína priônica celular PrPc. Apesar dos mecanismos envolvidos na neurodegeneração não estarem totalmente descritos, sabemos que envolvem múltiplos processos operando simultânea e sinergicamente no cérebro, incluindo degeneração espongiforme, alterações sinápticas, inflamação cerebral, morte neuronal e acúmulo de agregados proteicos. No Brasil, as DPr tornaram-se doenças de notificação compulsória ao Sistema de Informação de Agravos de Notificação (SINAN) a partir de 2005, sendo confirmados até 2020 mais de 400 casos de DPr, com aumento significativo de casos a partir do ano de 2012, principalmente nos estados de São Paulo, Minas Gerais e Paraná, provavelmente pela maior capacidade de diagnóstico destes estados. Dessa forma, uma compreensão mais abrangente destas doenças e sua epidemiologia pode ajudar no diagnóstico precoce e desenvolvimento de terapias muito necessárias para estas doenças devastadoras.
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