The present study was carried out to evaluate the occurrence of Salmonellae in raw broiler parts and to determine the antimicrobial resistance profile of the isolated strains. Twenty-four (39.3%) broiler parts samples were positive for Salmonella and twenty-five Salmonella strains were isolated, since two different serovars were detected in one single positive sample. Salmonella Enteritidis was the most prevalent serovar. Among Salmonella Enteritidis isolates, 95.2% belonged to Phage Type 4 (PT4) (20/21) and 4.8% to PT7 (1/21). Twenty-two (88%) strains of Salmonella were resistant to at least one antimicrobial agent, generating eight different resistance patterns. The S. Typhimurium (n: 1) and S. Hadar (n: 3) isolates presented multiple resistance. Three S. Enteritidis isolates were susceptible to all antimicrobials tested, two were resistant only to tetracycline. The high prevalence of Salmonella in the broiler parts strenghtens the importance of the use of good manufacturing practices (GMP), and HACCP. The results also emphasize the need for the responsible use of antimicrobials in animal production.

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RESUMOCampylobacter são microorganismos patogênicos associados com aves ou alimentos de origem avícola e sua importância está relacionada à alta prevalência de Campylobacter nos frangos de corte e suas carcaças, correlacionados com gastroenterite em humanos. Neste estudo, monitorou-se 22 lotes de frango de corte com idades entre 3 a 5 semanas na granja e 35 dias no abate e avaliou-se os métodos de pré-enriquecimento (PE) e isolamento direto (ID) para identificação de Campylobacter em swabs cloacais e carcaças de frango. Realizaram-se 22 análises de swabs cloacais pelo PE e pelo ID, 96 análises de carcaças pelo ID e, destas, 95 pelo PE. Para o isolamento direto a partir de swabs utilizou-se o ágar mCCDA acrescido de suplemento seletivo, acondicionado em embalagem não permeável e microaerofilia com mistura de gases (5% O 2 , 10% CO 2 e 85% N 2 ). Para o PE, os swabs foram inoculados em pool no caldo Bolton suplementado com antibióticos e 200 mg/ L de TTC, seguido de inoculação em ágar mCCDA, também em microaerofilia. As carcaças também foram analisadas para ambos os métodos, utilizando-se caldo Bolton no pré-enriquecimento, seguido de inoculação em mCCDA ou isolamento direto em ágar Bolton com TTC, sempre em microaerofilia. Não houve diferença significativa (p=1,00) entre os métodos de pré-enriquecimento e isolamento direto nas amostras de swabs e carcaças. Identificou-se 81,8% lotes positivos por ID e 77,3% pelo PE na análise dos swabs e 99,0% das carcaças pelo PE e 97,9% pelo ID. Os métodos de pré-enriquecimento e isolamento direto foram homogêneos e sensíveis para detecção de Campylobacter em amostras de swabs cloacais e carcaças de frango. Entretanto, pela praticidade e antecipação dos resultados em 24 horas, recomenda-se a utilização do método de isolamento direto.Descritores: Campylobacter, pré-enriquecimento, isolamento direto, swabs cloacais, carcaças de frangos. ABSTRACTCampylobacter are pathogenic microorganisms associated with poultry or poultry products. Its importance is related to high prevalence of Campylobacter in broiler flocks and in carcasses, which frequency is correlated to gastroenteritis in human. This study was realized in 22 broiler chicken flocks with from 3 to 5 weeks and the slaughter age of 35 days. Were evaluated the pre-enrichment (PE) and direct plating (DP) in the identification of Campylobacter in cloacae swabs and broiler carcasses. Were analyzed 22 cloacae swabs by PE and DP, 96 carcasses by DP, and of these, 95 by PE. In the DP, the swabs were plated directly onto selective modified agar (mCCDA) and placed in waterproof bags, under microaerophilia, with a gas mixture (5% O 2 , 10% CO 2 and 85% N 2 ). In the PE, they were pooled onto Bolton broth supplemented with antibiotics, 15 g/ L of agar, 0.5g/L of iron sulfate and 200 mg/L of TTC, followed by plating onto mCCDA, also under microaerophilia. Carcasses were assessed in both methods using Bolton broth in PE, followed by plating onto mCCDA, and DP onto Bolton agar with TTC. No statistically significant difference (p=1.00) was ob...

RESUMO A resistência antimicrobiana é um assunto amplamente estudado em todos os gêneros bacterianos, sobretudo em relação aos responsáveis por zoonoses, como o caso da Salmonella, a qual merece especial atenção, já que pode ser transmitida para os seres humanos. O presente estudo foi conduzido para verificar a ocorrência de resistência a agentes antimicrobianos em 22 cepas de Salmonella Hadar isoladas de carcaças congeladas de frango no Estado do Rio Grande do Sul, no período de maio de 1995 a abril de 1996. No presente estudo, os resultados indicaram que 100% das cepas de Salmonella Hadar apresentaram resistência a tetraciclina, estreptomicina e sulfazotrim, tendo também apresentado resistência em diferentes níveis ao ácido nalidíxico (86,36%), nitrofurantoína (18,18%) e cloranfenicol (4,54%). Todas as cepas de Salmonella Hadar apresentaram resistência a três ou mais agentes antimicrobianos, com cinco diferentes padrões de resistência. Os níveis de resistência observados enfatizam a necessidade do uso responsável dos agentes antimicrobianos na produção animal.

Forming a management board for a Protected Area (PA) is a democratic process which exercises social participation in public environmental management. Ilha dos Lobos Wildlife Refuge (WLR), in southern Brazil, had its management council formed in 2016, 33 years after its creation. The objective of this article was to characterize the board members’ profiles emphasizing their perception of the role of the management council and the challenges and opportunities of this PA. In order to achieve these goals, qualitative methodologies such as the application of 30 questionnaires with closed questions and nine semi-structured interviews with the board members were used. Data collection also involved bibliographical and documentary research. The general profile of the board members is of residents at the PA municipality, with a high level of education, and with previous experience in other councils. According to the interviewed members, the main role of a board member is to provide the management council with technical information, to help integrating society with the PA, and to spread the importance of the WLR. Regarding the main goals of the WLR, its importance as a refuge for sea lions and for scientific research was highlighted. As to the conflicts, the management council members mentioned the illegal fishing within the PA and the fishery interaction with sea lions as the main problems. In terms of future perspectives, the board members revealed the desire to develop sustainable tourism and to implement a management plan. In this context, the establishment of the management council of Ilha dos Lobos WLR represents a milestone in the history of participative management of this PA, consolidating a space for clarification, discussion and integration with the stakeholders of its area of influence. Keywords: Social participation, marine protected area, participatory governance, stakeholders.