Crocin is the main bioactive components of the saffron with neuro generative and protective activity, however, its activity in neurodegenerative disease in not fully elicited. So, the aim of the current study was to determine effects of the crocin on re exive motor behavior, depressive and serum antioxidant activities on cuprizone-induced (CPZ) model of multiple sclerosis (MS) mice. 40 male C57BL/6 mice were randomly assigned into 4 groups. Mice in the control group were treated with normal diet. In group 2, CPZ-induced demyelination was done by chew palate containing 0.2% (w/w) CPZ for 5 weeks. In group 3, normal diet was provided and mice orally received crocin (100 mg/kg) 3 times per week for 5 weeks. In group 4, mice feed CPZ containing diet and orally received crocin (100 mg/kg) three times per for 5 weeks. At the end of the study, re exive motor behavior and depressive tests were done. Also, serum and brain tissue antioxidant activity was determined. According to the data, crocin had positive effects on hind-limb foot angle, hind-and front-limb suspension, surface righting, grip strength and negative geotaxis while CPZ had adverse effect compare to control group (P < 0.05). Co-administration of the CPZ + crocin signi cantly decreased adverse effect of the CPZ on the re exive motor behavior tests (P < 0.05).CPZ signi cantly increased immobility time in the forced swimming test (FST), tail suspension test (TST) and crocin diminished it (P < 0.05). Co-administration of the CPZ + crocin signi cantly decreased adverse effect of the CPZ on immobility time (P < 0.05). CPZ decreased number of cross in open eld test (OFT) and spending time on rotarod and CPZ + crocin signi cantly lessened adverse effect of the CPZ (P < 0.05). CPZ signi cantly increased malondialdehyde (MDA) and decreased glutathione peroxidase (GPx), superoxide dismutase (SOD) and total antioxidant status (TAS) and these effects reversed by crocin in brain tissue and serum (P < 0.05). Co-administration of the CPZ + crocin signi cantly improved adverse effect of the CPZ on serum and brain tissue antioxidants (P < 0.05). These results suggested crocin has protective effect against on CPZ-induced MS in mice.
Crocin is the main bioactive components of the saffron with neuro generative and protective activity, however, its activity in neurodegenerative disease in not fully elicited. So, the aim of the current study was to determine effects of the crocin on reflexive motor behavior, depressive and serum antioxidant activities on cuprizone-induced (CPZ) model of multiple sclerosis (MS) mice. 40 male C57BL/6 mice were randomly assigned into 4 groups. Mice in the control group were treated with normal diet. In group 2, CPZ-induced demyelination was done by chew palate containing 0.2% (w/w) CPZ for 5 weeks. In group 3, normal diet was provided and mice orally received crocin (100 mg/kg) 3 times per week for 5 weeks. In group 4, mice feed CPZ containing diet and orally received crocin (100 mg/kg) three times per for 5 weeks. At the end of the study, reflexive motor behavior and depressive tests were done. Also, serum and brain tissue antioxidant activity was determined. According to the data, crocin had positive effects on hind-limb foot angle, hind- and front-limb suspension, surface righting, grip strength and negative geotaxis while CPZ had adverse effect compare to control group (P < 0.05). Co-administration of the CPZ + crocin significantly decreased adverse effect of the CPZ on the reflexive motor behavior tests (P < 0.05). CPZ significantly increased immobility time in the forced swimming test (FST), tail suspension test (TST) and crocin diminished it (P < 0.05). Co-administration of the CPZ + crocin significantly decreased adverse effect of the CPZ on immobility time (P < 0.05). CPZ decreased number of cross in open field test (OFT) and spending time on rotarod and CPZ + crocin significantly lessened adverse effect of the CPZ (P < 0.05). CPZ significantly increased malondialdehyde (MDA) and decreased glutathione peroxidase (GPx), superoxide dismutase (SOD) and total antioxidant status (TAS) and these effects reversed by crocin in brain tissue and serum (P < 0.05). Co-administration of the CPZ + crocin significantly improved adverse effect of the CPZ on serum and brain tissue antioxidants (P < 0.05). These results suggested crocin has protective effect against on CPZ-induced MS in mice.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.