BACKGROUND: Skin is the body's fi rst defence against direct exposure to variety of chemicals. Polycyclic aromatic hydrocarbons such as 3-methylcholanthrene (3-MC) are common in polluted urban air and have a potential of producing harmful effects. Moreover, their late effects can occur months or years after exposure. OBJECTIVES: We aimed to investigate the long-term effects of 3-MC induced dermal toxicity on the expression of markers of apoptosis, pleiotropic cytokines, and oxidative stress and to determine the protective effect of cisplatin. METHODS: Groups were designed as control (group 1), 3-MC applied (group 2) and 3-MC+cisplatin applied mice (group 3). Cutaneous expressions of TGFβ, PDGFA, PDGFC, bFGF, PDGFRα, USP28, and Ki67 were evaluated with qPCR. Total oxidant (TOS), total antioxidant (TAS) and oxidative stress index (OSI) values were determined in liver and kidney tissues. RESULTS: The expression levels of TGFβ, PDGFRα, USP-28, Ki67, and PDGFA were decreased signifi cantly in MC applied groups. Renal TAS levels were signifi cantly lower in group-3. Liver and kidney OSI values were increased in both groups 2 and 3. CONCLUSION: The results indicated that low dose 3-MC caused oxidative stress and downregulated apoptotic and cytokine markers in the long term and cisplatin had no ameliorative effects on this degeneration processes (Tab.
This study aimed to evaluate the protective effect of krill oil against nephrotoxicity caused by gentamicin. Distilled water was given orally to the control and second groups (GI) for seven days while 500 mg/kg krill oil was given to the third (GII), fourth (GIII) groups. In addition, isotonic saline was administered subcutaneously to the control and GIII groups throughout the study, while 80 mg/kg gentamicin was administered to the GI, and GII groups. Alanine aminotransferase (ALT) and gamma glutamyltransferase (GGT) peptidase, total cholesterol, urea, and creatinine levels in plasma and, malondialdehyde (MDA) and total antioxidant status (TAS) levels in both plasma and kidney tissue supernatant were evaluated. Histopathological changes in tubules and glomeruli and vascular changes were evaluated by scoring. Urea level and ALT activity were found to be significantly lower in the GII and GIII groups compared to the GI group (p<0.001; p≤0.001). As a result, it was observed that degenerative damage and glomerular changes in the tubule at the histological level mediated by oxidative stress were consistent with the increase in ALT, urea, and MDA levels. In this respect, it is suggested that krill oil can be used as a nephroprotective food supplement to contribute to treatment in cases of toxicity.
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