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Abstract.-Crude, aqueous, ethanol extracts and essential oils of aerial parts of Dysphania ambrosioides were investigated for their in vitro antimicrobial properties. The plant was collected from Agouray village near Meknès city of Morocco. D. ambrosioides is widely used as traditional folk medicine in Morocco. The main components in the oil were α-terpinene (61.04%), 4-carene (13.55%) and pcymene (12.94%). The antimicrobial activity was evaluated against 12 microorganisms using the disc-diffusion assay, well in agar method, minimal inhibition concentration (MIC) and minimal bactericidal concentration (MBC) values. The different extracts tested had no antimicrobial effect against the test microorganisms whereas the essential oil had inhibitory effects on the growth of bacteria, yeasts and fungi.

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The aim of this work is to determine the chemical composition of the essential oil of Origanum compactum, from the region of Meknes (Morocco), and their antimicrobial effect, and on the other hand, to carry out a comparison between the chemical composition of this essential oil with the six regions of Morocco: Larache, Chefchaouen, Al Hoceïma, Tetouan, Taounate and Rabat. The essential oils of Origanum compactum thus obtained were analyzed and identified by gas chromatography coupled with mass spectrometry (GC/MS). To determine the similarities and dissimilarities between the chemical compositions of the essential oil of Origanum compactum from the Meknes region with the other six regions of Morocco, we performed the principal component analysis (ACP). Regarding antimicrobial activity, the essential oils of Origanum compactum have shown significant inhibitory activity against the bacteria and molds studied.