1. This study was conducted to determine the effects of heat stress on fearfulness, leucocyte components, oxidative stress and lipid peroxidation in two commercial broiler strains, Cobb (C) and Ross (R). 2. At 36 and 37 d of age birds were exposed to 38 +/- 1 degree C for 3 h. Rectal temperatures, duration of tonic immobility (TI), haematocrit values, proportions of leucocyte components (heterophil, lymphocyte, basophil, eosinophil, monocyte), malondialdehyde (MDA) concentrations and antioxidant enzyme activities (CAT, SOD, GPx) of all the birds were determined, before and after heat treatment. 3. Rectal temperatures increased and haematocrit values decreased in birds exposed to heat stress. Heat stress caused a significant increase in heterophil/lymphocyte and in basophil ratios. 4. Exposing birds to heat stress increased duration of TI, suggesting heat-stressed birds tended to be more fearful. 5. Heat stress resulted in a significant Genotype x Treatment interaction for MDA concentration. CAT, SOD and GPx activities; MDA concentrations in heat-stressed R strain birds were greater than in heat-stressed C strain birds.

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1. This study was conducted to determine metabolic and physiological responses of 2 commercial broiler strains, Hubbard (H) and Cobb (C), exposed to an ambient temperature of 38 degrees +/- 1 degree C for 2 h at 14 and 15 d of age. 2. Exposure to high temperature at an early age resulted in weight loss in strain C, which was not compensated for by 35 d of age but there was no weight loss in strain H. 3. Exposure of broilers to heat stress (38 degrees +/- 1 degree C) at 35 d of age resulted in an increase in rectal temperature, regardless of previously high temperature experience but acid-base balance and haematocrit values were not affected by heat stress. 4. Malondialdehyde concentration was higher in unexposed birds than in previously exposed ones and did not significantly differ between strains.

1. The present study was conducted to determine if detrimental effects of high temperature on broiler performance could be overcome by short-term fasting and to examine the effect of food withdrawal on the responses of two commercial strains (S1 and S2). 2. An experimental design was used with three feeding groups and two strains. Birds in the control (C) group were fed ad libitum during the experimental period (0 to 6 weeks), while feed was withdrawn in the other groups between 10:00 and 16:00 h during weeks 5 and 6 (F1) or week 6 (F2), respectively. After standard brooding for the first 3 weeks temperature and humidity varied according to outside climate between 4 and 6 weeks of age. 3. Feeding strategy significantly affected body weight at week 6 (BW6) and daily weight gain during week 6 (DWG5-6). F2 gave the best results in BW6 and DWG5-6. Strain effect was significant on DWG4-5 and DWG5-6; S1 had higher DWG4-5 and S2 gained more at the last stage. S1C broilers had greater BW6 than S2C but the S2 strain gave a better response to short-term fasting compared with S1 and this resulted in significant strain by feeding interaction effect on BW6 and DWG5-6. 4. It was shown that short-term fasting was an effective method for controlling rectal temperature (RT) of broilers at 5 and 6 weeks of age. 5. Total food consumption (FCO-6), food conversion ratio (FCRO-6), mortality, and carcase characteristics were not affected by short-term fasting in the experimental conditions. 6. In conclusion, it can be recommended that broiler producers may practise 6 h of fasting during the hot period of the day without any adverse effect on slaughter weight and FCR when the birds are close to the average slaughter weight and a heat wave is expected. Strain differences in RT measurements also indicate the importance of choosing strains to be used in hot and warm climate conditions with attention to the thermoregulation ability of broilers.

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Chemical vapor deposition (CVD) has emerged as the most promising technique towards manufacturing of large area, high quality graphene. Characterization, understanding, and controlling of various structural defects in CVD-grown graphene are essential to realize its true potential for real-world applications. We report a new method for in situ chemical probing of vacancy defects in CVD-grown graphene at room temperature. Our approach is based on a solid-gas phase reaction that occurs selectively in graphene vacancy defect regions such as holes and cracks. Our new probing technique has a unique combination of the following advantages: (1) no exposure to liquids; (2) non-damaging in situ probing; (3) high selectivity, sensitivity, and reliability towards vacancy defects; (4) simplicity and scalability. By focusing on hexagonal graphene domains, we have made the following findings: (1) the nucleation centers of graphene domains are favorable locations of hole defects. (2) The lengthy electron-beam irradiation at very low energy (3.5 keV) could etch the graphene. (3) Graphene cracks often kink at the angle of primarily 150° or 120°. (4) There exist complex graphene cracks such as cracks with clock-hands patterns, and cracks with snowflake-like branched structures. (5) There exist discontinuous cracks in some graphene domains, where hole defects are oriented along a straight or curved line. Such discontinuous cracks may arise from the ductile fracture of graphene. In addition, we have shown that our method is also applicable to chemical probing of vacancy defects such as holes, continuous and discontinuous cracks in CVD-grown monolayer polycrystalline graphene films on copper. Our study also suggests that the copper grain and copper grain boundary play significant roles in formation and distribution of graphene vacancy defects.

Sesame seeds can be contaminated with aflatoxins during growth or storage. Therefore, tahini, a sesame seed-based product, has potential risk of aflatoxin contamination. In this study, a total of 104 tahini samples collected from Central Anatolian region of Turkey were analysed for aflatoxin contamination. Analysis of samples was carried out by high-performance liquid chromatography with fluorescence detection after immunoaffinity column clean-up, with recoveries ranging from 70.3 to 82.4%. In terms of incidence and quantity, aflatoxin B 1 was the predominant mycotoxin detected in 15 samples, with contamination levels ranging from 0.31 to 2.53 µg/kg. The mean level of total aflatoxins in contaminated samples was found to be 1.17±0.55 µg/kg. Our data revealed that aflatoxin B 1 and total aflatoxins levels determined in tahini samples were within the Turkish Food Codex limits. The incidence of contaminated tahini samples indicated that a regular surveillance program should be established to monitor the occurrence of aflatoxins in tahini.