Purpose:To evaluate that Connexin (Cx43) plays a role in lesions after hepatic ischemia/reperfusion (IR) injury.Methods:We use Cx43 deficient model (heterozygotes mice) and compared to a wild group. The groups underwent 1 hour ischemia and 24 hours reperfusion. The heterozygote genotype was confirmed by PCR. We analyzed the hepatic enzymes (AST, ALT, GGT) and histology.Results:The mice with Cx43 deficiency showed an ALT mean value of 4166 vs. 307 in the control group (p<0.001); AST mean value of 7231 vs. 471 in the control group (p<0.001); GGT mean value of 9.4 vs. 1.7 in the control group (p=0.001); histology showed necrosis and inflammation in the knockout group.Conclusions:This research demonstrated that the deficiency of Cx43 worses the prognosis for liver injury. The topic is a promising target for therapeutics advancements in liver diseases and procedures.

IntroductionPatients with liver cirrhosis are at a higher risk of hospitalization. The present review aimed to assess the risk of thromboembolism and its burden on hospitalized cirrhotic patients.Materials and methodsA systematic review (PROSPERO: CRD42021256869) was conducted in PubMed, Embase, Cochrane, Lilacs, and a manual search of references. It evaluated studies that compare cirrhotic patients with venous thromboembolism (VTE) with cirrhotic patients without VTE or studies that compare cirrhotic patients with non-cirrhotic patients. No restrictions were set for the date of publication or language. The last search was conducted in June 2021.ResultsAfter selection, 17 studies were included from an initial search of 5,323 articles. The chronic liver disease etiologies comprise viral, alcohol, autoimmune, NASH (non-alcoholic steatohepatitis), cryptogenic, hemochromatosis, cholestasis, and drug-related. The included studies were conflicted regarding the outcomes of VTE, pulmonary embolism, or bleeding. Patients with cirrhosis associated with VTE had prolonged length of hospital stay, and patients with cirrhosis were at higher risk of portal thrombosis.ConclusionIn-hospital cirrhotic patients are a heterogeneous group of patients that may present both thrombosis and bleeding risk. Clinicians should take extra caution to apply both prophylactic and therapeutic anticoagulation strategies.Systematic review registration[https://www.crd.york.ac.uk/PROSPERO/], identifier [CRD42021256869].

INTRODUÇÃO 1.1 Fisiopatologia da isquemia e reperfusão 1.1.1 Citocinas envolvidas e recrutamento de neutrófilos na IR 1.1.2 Mediadores da Matriz Extracelular (MEC) e células estreladas hepáticas (HSCs) 1.2 Comunicação celular 1.2.1 Conexinas Conexina 43 1.2.2 Interações celulares e expressão de conexinas no fígado 1.3 Justificativa do estudo 2 OBJETIVO 3 MATERIAIS E MÉTODOS 3.1 Animais geneticamente modificados 3.2 Genotipagem 3.3 Delineamento experimental 3.4 Análise bioquímica 3.5 Exame histopatológico do fígado 3.6 Análise estatística