The application of various microscopy methods-from stereomicroscopy to luminescence microscopy, microspectrofluorimetry and laser-scanning confocal microscopy-has been considered as an approach to model the cell-cell contacts and interactions in allelopathy. It bases on the direct observations of both secretions released from allelopathic species and the interaction(s) with the cell acceptors as biosensors (unicellular plant generative and vegetative microspores). Special attention was paid to the interactions with pigmented and fluorescing components of the secretions released by the cell donors from allelopathically active plant species. Colored allelochemicals are considered as histochemical dyes for the analysis of cellular mechanisms at the allelopathic contacts. IntroductionThe study of cell-cell interactions in allelopathy has been achieved using various types of microscopic methods as has been shown for analysis of pollen-pollen interactions analyzed by microspectrofluorimetry Melnikova 1996, 1999) or laser-scanning confocal microscopy (Roshchina et al. 2007(Roshchina et al. , 2009a. The technique may be suitable for the investigation of allelopathic mechanisms when one cell (a donor cell) releases a molecule that is received by another cell (an acceptor cell) belonging to different species. For this aim it should consider the microscopy for the observation of plant cell, viz.
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