All Australian land mammals, reptiles, and birds weighing more than 100 kilograms, and six of the seven genera with a body mass of 45 to 100 kilograms, perished in the late Quaternary. The timing and causes of these extinctions remain uncertain. We report burial ages for megafauna from 28 sites and infer extinction across the continent around 46,400 years ago (95% confidence interval, 51,200 to 39,800 years ago). Our results rule out extreme aridity at the Last Glacial Maximum as the cause of extinction, but not other climatic impacts; a "blitzkrieg" model of human-induced extinction; or an extended period of anthropogenic ecosystem disruption.

Aim  To assess whether eight factors thought to be involved in the extinction process can explain the pattern of recent decline in Australia's mammal fauna. Location  Australia. Methods  We compiled the first comprehensive lists of mammal species extant at the time of European settlement in each of Australia's 76 mainland regions, and assigned a current conservation status to each species in each region to derive an index of faunal attrition. We then sought to explain the observed region‐to‐region variation in attrition (the dependent variable) by building a series of models using variables representing the eight factors. Results  A strong geographically based pattern of attrition emerged, with faunal losses being greatest in arid regions and least in areas of high rainfall. The Akaike information criterion showed support for one model that explained 93% of the region‐to‐region variation in attrition. Its six variables all made independent contributions towards explaining the observed variation. Two were environmental variables, namely mean annual rainfall (a surrogate for regional productivity) and environmental change (a measure of post‐European disturbance). The other four were faunal variables, namely phylogenetic similarity, body‐weight distribution, area (as a surrogate for extent of occurrence), and proportion of species that usually shelter on the ground (rather than in rock piles, burrows or trees). Main conclusions  In combination with historical evidence, the analysis provides an explicit basis for setting priorities among regions and species. It also shows that the long‐term recovery of populations of many species of Australian mammals will require introduced predator suppression as well as extensive habitat management that includes controlling feral herbivores. Specifically, habitat management should restore aspects of productivity relevant to the types of species at risk and ensure the continual availability of suitable refuges from physiological stressors.

This paper attempts to identify and explain patterns in the biogeography of Australia’s indigenous terrestrial mammals at the time of European settlement (before modern extinctions), and also compares species’ pre-European and current status by region. From subfossil, historical and contemporary sources, we compiled data on the past geographic range and present status of mammals for Australia’s 85 biogeographic regions. Of the 305 indigenous species originally present, 91 have disappeared from at least half of the bioregions in which they occurred before European settlement. Thirty-nine extant species ‘persist’ in less than 25% of their original bioregions; 28 of these are marsupials and 11 are rodents. Twenty-two of the original 305 species are extinct, a further eight became restricted to continental islands, and 100 have become extinct in at least one bioregion. Over the same period, 26 species of exotic mammals established wild populations and now occupy from one to 85 bioregions. When we classified the bioregions in terms of their original species composition, the 3-group level in the dendrogram approximated the Torresian, Eyrean and Bassian subregions proposed by Spencer in 1898, while the 4-group level separated southern semiarid Eyrean bioregions, including those in south-west Australia, from the arid Eyrean bioregions. The classification dendrogram showed geographically (and statistically) discrete clustering down to the 19-group level, suggesting that all four subregions can be further divided on the basis of their mammal faunas. Variation partitioning showed 66% of the biogeographical pattern can be explained by environmental factors (related to temperature and precipitation), the spatial position of each bioregion (a third-order polynomial of latitude and longitude), the area of each bioregion, and the richness of species in each bioregion. In addition to the marked distributional changes that indigenous mammals have experienced over the last 200 years, the 49% of variation explainable by temperature and precipitation implies further shifts with global climate change.

Owing to exceptional biomolecule preservation, fossil avian eggshell has been used extensively in geochronology and palaeodietary studies. Here, we show, to our knowledge, for the first time that fossil eggshell is a previously unrecognized source of ancient DNA (aDNA). We describe the successful isolation and amplification of DNA from fossil eggshell up to 19 ka old. aDNA was successfully characterized from eggshell obtained from New Zealand (extinct moa and ducks), Madagascar (extinct elephant birds) and Australia (emu and owl). Our data demonstrate excellent preservation of the nucleic acids, evidenced by retrieval of both mitochondrial and nuclear DNA from many of the samples. Using confocal microscopy and quantitative PCR, this study critically evaluates approaches to maximize DNA recovery from powdered eggshell. Our quantitative PCR experiments also demonstrate that moa eggshell has approximately 125 times lower bacterial load than bone, making it a highly suitable substrate for high-throughput sequencing approaches. Importantly, the preservation of DNA in Pleistocene eggshell from Australia and Holocene deposits from Madagascar indicates that eggshell is an excellent substrate for the long-term preservation of DNA in warmer climates. The successful recovery of DNA from this substrate has implications in a number of scientific disciplines; most notably archaeology and palaeontology, where genotypes and/or DNA-based species identifications can add significantly to our understanding of diets, environments, past biodiversity and evolutionary processes.

Although the temporal overlap between human dispersal across Australia and the disappearance of its largest animals is well established, the lack of unambiguous evidence for human–megafauna interactions has led some to question a human role in megafaunal extinction. Here we show that diagnostic burn patterns on eggshell fragments of the megafaunal bird Genyornis newtoni, found at >200 sites across Australia, were created by humans discarding eggshell in and around transient fires, presumably made to cook the eggs. Dating by three methods restricts their occurrence to between 53.9 and 43.4 ka, and likely before 47 ka. Dromaius (emu) eggshell occur frequently in deposits from >100 ka to present; burnt Dromaius eggshell first appear in deposits the same age as those with burnt Genyornis eggshell, and then continually to modern time. Harvesting of their eggs by humans would have decreased Genyornis reproductive success, contributing to the bird's extinction by ∼47 ka.

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-The hills and ranges of the Pilbara contain large numbers of caves, but only a miniscule proportion of these holds mammal remains that can be used to reconstruct the original (i.e. pre-European) fauna. During two fi eld seasons in 1985 and 2004, only 12 sites with bones were discovered. Material from three other sites lodged in the collections of the Western Australian Museum was also used. Thirty-six native mammal species plus the introduced House Mouse were identifi ed from cave surface remains. Compared with the fauna known from live-caught specimen records and the results of the Pilbara Biodiversity Survey, rodent species are comprehensively represented among the remains, dasyurid marsupials and bandicoots are moderately well represented, macropodoids are poorly represented, and bats (except two cave-roosting species) hardly represented at all. Results of this study indicate that the Central Rock-rat (Zyzomys pedunculatus), and probably the Golden-backed Tree-rat (Mesembriomys macrurus), originally occurred throughout the Pilbara ranges. Chuditch (Dasyurus geoffroii) is recorded from the Pilbara for the fi rst time, suggesting that it may have occurred in the lowlands, while Northern Quoll (D. hallucatus) occupied the ranges. New records of Melomys burtoni and Pseudomys fi eldi represent substantial extensions of geographic range. Overall, these results confi rm the Pilbara as the western outpost of several northern mammal species, but show that the original fauna is less distinctive than previously thought, with a higher proportion of species widespread in the arid zone.