We study the effect of salt concentration on the ionic conductance and translocation of single DNA molecules through nanocapillaries made out of quartz glass. DNA translocation experiments were performed in aqueous solution for concentrations of KCl between 10 mM and 2 M while ion conductance was characterized from 1 mM to 2 M KCl concentration. Here, we develop a model for the conductance of conical nanocapillaries taking into consideration the surface charge of the quartz glass. We demonstrate that the conductance of our nanocapillaries shows similar behavior to silicon oxide nanopores at low and high KCl concentrations. Finally, we show that DNA translocations in high KCl concentrations (400 mM-2 M) cause a reduction in the ionic current. In contrast, DNA translocations at low KCl concentrations (10-300 mM) lead to increases in the ionic current. Our new results, which until now have not been shown for nanocapillaries, can be well understood with an adapted model.

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Introduction Understanding the impact of lockdown upon resistance training (RT), and how people adapted their RT behaviours, has implications for strategies to maintain engagement in similar positive health behaviours. Further, doing so will provide a baseline for investigation of the long-term effects of these public health measures upon behaviours and perceptions, and facilitate future follow-up study. Objectives To determine how the onset of coronavirus (COVID-19), and associated ‘lockdown’, affected RT behaviours, in addition to motivation, perceived effectiveness, enjoyment, and intent to continue, in those who regularly performed RT prior to the pandemic. Methods We conducted an observational, cross-sectional study using online surveys in multiple languages (English, Danish, French, German, Italian, Portuguese, Slovakian, Swedish, and Japanese) distributed across social media platforms and through authors’ professional and personal networks. Adults ( n = 5389; median age = 31 years [interquartile range (IQR) = 25, 38]), previously engaged in RT prior to lockdown (median prior RT experience = 7 years [IQR = 4, 12]) participated. Outcomes were self-reported RT behaviours including: continuation of RT during lockdown, location of RT, purchase of specific equipment for RT, method of training, full-body or split routine, types of training, repetition ranges, exercise number, set volumes (per exercise and muscle group), weekly frequency of training, perception of effort, whether training was planned/recorded, time of day, and training goals. Secondary outcomes included motivation, perceived effectiveness, enjoyment, and intent to continue RT. Results A majority of individuals (82.8%) maintained participation in RT during-lockdown. Marginal probabilities from generalised linear models and generalised estimating equations for RT behaviours were largely similar from pre- to during-lockdown. There was reduced probability of training in privately owned gyms (~ 59% to ~ 7%) and increased probability of training at home (~ 18% to ~ 89%); greater probability of training using a full-body routine (~ 38% to ~ 51%); reduced probability of resistance machines (~ 66% to ~ 13%) and free weight use (~ 96% to ~ 81%), and increased probability of bodyweight training (~ 62% to ~ 82%); reduced probability of moderate repetition ranges (~ 62–82% to ~ 55–66%) and greater probability of higher repetition ranges (~ 27% to ~ 49%); and moderate reduction in the perception of effort experienced during-training ( r = 0.31). Further, individuals were slightly less likely to plan or record training during lockdown and many changed their training goals. Additionally, perceived effectiveness, enjoyment, and likelihood of continuing current training were all lower during-lockdown. Conclusions Those engaged in RT prior to lockdown these behaviours with only slig...

Objectives: To determine how the onset of COVID-19, and the associated ‘lockdown’, affected RT behaviours, in addition to motivation, perceived effectiveness, enjoyment, and intent to continue, in those who regularly performed resistance training RT prior to the pandemic. Design: Observational, cross-sectional. Setting: Online surveys in multiple languages (English, Danish, French, German, Italian, Portuguese, Slovakian, Swedish, and Japanese) distributed across social media platforms and through authors professional and personal networks. Participants: Adults (n = 5389 after data cleaning; median age = 31 years [IQR = 25, 38]), who were previously engaged in RT prior to lockdown (median prior RT experience = 7 years [IQR = 4, 12]). Main outcomes: Self-reported RT behaviours including: continuation of RT during lockdown, location of RT, purchase of specific equipment for RT, method of training (e.g. alone, supervision etc.), full-body or split routine, types of training, repetition ranges, exercise number, set volumes (per exercise and muscle group), weekly frequency of training, perception of effort, whether training was planned/recorded, time of day, and training goals. Secondary outcomes included motivation, perceived effectiveness, enjoyment, and intent to continue RT. Results: A majority of individuals (82.8%) maintained participation in some form of RT during- lockdown. Marginal probabilities from generalised linear models and generalised estimating equations of engaging in certain RT behaviours were largely similar from pre- to during- lockdown (particularly numbers of exercises, sets per exercise or muscle group, and weekly frequencies). There was reduced probability of training in privately owned gyms and increased probability of training at home; greater probability of training using a full-body routine; reduced probability of resistance machines use and increased probability of free weight and bodyweight training; reduced probability of moderate repetition ranges and greater probability of higher repetition ranges; and reduction in the perception of effort experienced during- training. Further, individuals were slightly less likely to plan or record training during- lockdown and many changed their training goals as a result of lockdown. Conclusions: Those engaged in RT prior to lockdown appeared mostly able to maintain these behaviours with only slight adaptations in both the location and types of training performed. However, people employed less effort, and motivation, perceived effectiveness, enjoyment, and likelihood of continuing current training all appeared lower during- lockdown. These results have implications for strategies to maintain engagement in positive health behaviours such as RT during- restrictive pandemic-related public health measures.

Background: Physical activity (PA) promotes health and well-being. For students, university represents a transitional period, including increased independence over lifestyle behaviors, in addition to new stressors and barriers to engaging in PA. It is, therefore, important to monitor PA trends in students to gain a greater understanding about the role it might play in physical and mental well-being, as well as other factors, such as attainment and employability. Methods: Cross-sectional surveys were conducted in 2016 in Scottish universities and colleges, and in 2017 in universities and colleges across the United Kingdom, and the data were pooled for the present study (N = 11,650). Cumulative ordinal logistic regression was used to model the association between PA levels and mental and personal well-being, social isolation, and perceptions of academic attainment and employability. Results: Only 51% of the respondents met the recommended levels of moderate to vigorous PA per week. There was a linear relationship between PA levels and all outcomes, with better scores in more active students. Conclusions: UK university students are insufficiently active compared with the general population of 16- to 24-year olds. Yet, students with higher PA report better outcomes for mental and personal well-being, social isolation, and perceptions of academic attainment and employability.

Heart rate, energy expenditure, and affective responses from children participating in trampoline park sessions compared with traditional extra-curricular sports clubs. SUMMARY OF KEY FINDINGS Lack of physical activity is widely becoming a concern in younger populations. Inactivity has negative associations with quality of life, in addition to children's academic, social, and physical skills. Further, it may predispose them to poorer health later in life. For most children physical education and extracurricular activities delivered through school are the most apparent opportunities for engagement in physical activity.  However, opportunities for physical activity exist outside of the school environment per se and a place that has seen an increase in popularity in recent years are trampoline parks. The emotional state (feelings) of a child during physical activity is thought to be related to their enjoyment and potentially adherence. Trampoline parks are typically thought of as being exciting adventure based activity and so it was of interest to examine both the heart rate and energy expenditure, in addition to affect, of children taking part in both extracurricular sports (football and netball) compared to a trampoline park session. A group of 26 children (aged 6-11 years, 16 girls and 10 boys) took part in the study.They wore heart rate belts during the activities and gave a rating of their feelings after them too. Data was collected from 3 sessions of both extracurricular sports in addition to trampoline park sessions. The results showed heart rate and energy expenditure were higher during trampoline park sessions, though both activities were classed as moderate-vigorous physical activity. Further, both activities elicited feelings of 'excitement'. Both extracurricular sports clubs and trampoline parks may be viable options for children to engage in physical activity that is sufficiently 'intense' and related to positive feelings. SCIENTIFIC ABSTRACTBackground: Lack of physical activity (PA) is becoming an issue in younger populations. Trampoline parks are newly popular environments for PA yet research on their use is scarce. Thus the present study compared heart rate, energy expenditure, and affective responses in children participating in trampoline park sessions compared with extracurricular sports clubs. Methods: Children (aged 6-11 years; n = 16 females, n = 10 males) participated in 3 trampoline park sessions and 3 extracurricular sports club sessions lasting ~45 minutes over 3 weeks. Heart rate, energy expenditure, and affective responses through the circumplex model, were measured. Results: Both conditions elicited moderate-vigorous PA. Average heart rate (mean difference [95%CIs] = 27.6 fc [23.5 to 31.8]), peak heart rate (mean difference [95%CIs] = 24.2 fc [20.8 to 27.6]) and energy expenditure (mean difference [95%CIs] = 3.2 kcals.min -1 [2.7 to 3.6]) were all significantly higher for the trampoline sessions. Affective responses for both conditions elicited feelings of 'excitement'. Conclusio...

This paper explores ecological perspectives of human activity in the use of digital musical instruments and assistive technology. While such perspectives are relatively nascent in DMI design and evaluation, ecological frameworks have a long-standing foundation in occupational therapy and the design of assistive technology products and services. Informed by two case studies, the authors' critique, compare and marry concepts from each domain to guide future research into accessible music technology.The authors discover that ecological frameworks used by occupational therapists are helpful in describing the nature of individual impairment, disability and situated context. However, such frameworks seemingly flounder when attempting to describe the personal value of music-making.

Visually impaired and blind (VIB) people as a community face several access barriers when using technology. For users of specialist technology, such as digital audio workstations (DAWs), these access barriers become increasingly complex—often stemming from a vision-centric approach to user interface design. Haptic technologies may present opportunities to leverage the sense of touch to address these access barriers. In this article, we describe a participant study involving interviews with twenty VIB sound creatives who work with DAWs. Through a combination of semi-structured interviews and a thematic analysis of the interview data, we identify key issues relating to haptic audio and accessibility from the perspective of VIB sound creatives. We introduce the technical and practical barriers that VIB sound creatives encounter, which haptic technology may be capable of addressing. We also discuss the social and cultural aspects contributing to VIB people’s uptake of new technology and access to the music technology industry.