In this paper, an irregular displacement-based lensless wide-field microscopy imaging platform is presented by combining digital in-line holography and computational pixel super-resolution using multi-frame processing. The samples are illuminated by a nearly coherent illumination system, where the hologram shadows are projected into a complementary metal-oxide semiconductor-based imaging sensor. To increase the resolution, a multi-frame pixel resolution approach is employed to produce a single holographic image from multiple frame observations of the scene, with small planar displacements. Displacements are resolved by a hybrid approach: (i) alignment of the LR images by a fast feature-based registration method, and (ii) fine adjustment of the sub-pixel information using a continuous optimization approach designed to find the global optimum solution. Numerical method for phase-retrieval is applied to decode the signal and reconstruct the morphological details of the analyzed sample. The presented approach was evaluated with various biological samples including sperm and platelets, whose dimensions are in the order of a few microns. The obtained results demonstrate a spatial resolution of 1.55 µm on a field-of-view of ≈30 mm2.
The current work describes the use of multidimensional Euclidean geometric distance (EGD) and Bayesian methods to characterize and classify the sky and cloud patterns present in image pixels. From specific images and using visualization tools, it was noticed that sky and cloud patterns occupy a typical locus on the redgreen-blue (RGB) color space. These two patterns were linearly distributed parallel to the RGB cube's main diagonal at distinct distances. A characterization of the cloud and sky patterns EGD was done by supervision to eliminate errors due to outlier patterns in the analysis. The exploratory data analysis of EGD for sky and cloud patterns showed a Gaussian distribution, allowing generalizations based on the central limit theorem. An intensity scale of brightness is proposed from the Euclidean geometric projection (EGP) on the RGB cube's main diagonal. An EGD-based classification method was adapted to be properly compared with existing ones found in related literature, because they restrict the examined color-space domain. Elimination of this limitation was considered a sufficient criterion for a classification system that has resource restrictions. The EGD-adapted results showed a correlation of 97.9% for clouds and 98.4% for sky when compared to established classification methods. It was also observed that EGD was able to classify cloud and sky patterns invariant to their brightness attributes and with reduced variability because of the sun zenith angle changes. In addition, it was observed that Mie scattering could be noticed and eliminated (together with the reflector's dust) as an outlier during the analysis. Although Mie scattering could be classified with additional analysis, this is left as a suggestion for future work.
Laser photocoagulation is currently the standard treatment for sight-threatening diseases worldwide, namely diabetic retinopathy and retinal vein occlusions. The slit lamp biomicroscope is the most commonly used device for this procedure, specially for the treatment of the eye periphery. However, only a small portion of the retina can be visualized through the biomicroscope, complicating the task of localizing and identifying surgical targets, increasing treatment duration and patient discomfort. In order to assist surgeons, we propose a method for creating intraoperative retina maps for view expansion using a slit-lamp device. Based on the mosaicking method described by Richa et al, 2012, the proposed method is a combination of direct and feature-based methods, suitable for the textured nature of the human retina. In this paper, we describe three major enhancements to the original formulation. The first is a visual tracking method using local illumination compensation to cope with the challenging visualization conditions. The second is an efficient pixel selection scheme for increased computational efficiency. The third is an entropy-based mosaic update method to dynamically improve the retina map during exploration. To evaluate the performance of the proposed method, we conducted several experiments on human subjects with a computer-assisted slit-lamp prototype. We also demonstrate the practical value of the system for photo documentation, diagnosis and intraoperative navigation.
This paper presents a systematic literature review concerning 3D segmentation algorithms for computerized tomographic imaging. This analysis covers articles published in the range 2006-March 2018 found in four scientific databases (Science Direct, IEEEXplore, ACM, and PubMed), using the methodology for systematic review proposed by Kitchenham. We present the analyzed segmentation methods categorized according to its application, algorithmic strategy, validation, and use of prior knowledge, as well as its general conceptual description. Additionally, we present a general overview, discussions, and further prospects for the 3D segmentation methods applied for tomographic images.
User satisfaction analyses in synchronous telemedicine and teleconsultation environments have been widely performed and generally show satisfied users. In the field of asynchronous telemedicine, however, satisfaction studies were performed only in one single location or with a restricted set of users. With the aim of offering an exemplar evaluation of the impact of the statewide use of a large-scale asynchronous telemedicine network on the satisfaction of the involved users, this study presents the results obtained from a survey of the perceived quality of the service by both patients and healthcare staff. For this purpose, a survey with satisfaction questionnaires was performed with 564 patients from seven upstate municipalities and 56 healthcare professionals from 46 municipalities, using a methodology from the process improvement field. The collected data were quantified and underwent statistical analysis, which showed a clear perception of the improvement in the quality of service by both patients and healthcare professionals. The present findings also showed that both patients and healthcare professionals felt that introducing these new technologies was a positive step, even in upstate areas and when they involved great changes in the usual processes of primary care.
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