A bstract Aim To determine the presence of Streptococcus mutans (S. mutans) and Streptococcus sobrinus (S. sobrinus) and their association with extrinsic and intrinsic variables in 6–18-month-old infants. Methods This was an analytical, cross-sectional study of 65 6–18-month-old infants who visited the Centers for Early Childhood in Buenos Aires City. Three groups were established according to the presence of teeth—group I (GI)—edentulous infants, group II (GII)—infants with 1–8 teeth, and group III (GIII)—infants with 9–16 teeth. Data on the variables, diet, use of artificial teats, and oral hygiene were gathered using a self-administered questionnaire. An oral examination was performed according to the International Caries Detection and Assessment System (ICDAS II) criterion. A saliva sample was taken by aspiration with a sterile plastic syringe. Cariogenic Streptococci (CS) were counted using the adherence test in modified gold broth (AT-MGB). Molecular detection and quantification were performed by quantitative polymerase chain reaction (qPCR) (gtfB, gtfT, and tuf). Results A total of 12% of infants received oral hygiene, 38% used bottles, 30% used pacifiers, and 55% had sugar intake. S. sobrinus and S. mutans were detected in 57.1 and 28.6% of the children with caries, respectively. Groups I, II, and III had CS counts of log 2, 3.4, and 3.7, respectively. S. sobrinus was detected in 26.7% of GI, 52.9% of GII, and 85.7% of GIII, while S. mutans was detected in 13.3%, 35.3%, and 57.7%, respectively. Conclusion The prevalence of S. sobrinus was higher than S. mutans in all groups. The presence of CS was significantly associated with sugar intake. No association was found between S. mutans and S. sobrinus and the presence of caries, hygiene habits, or use of artificial teats. Clinical Significance This study supports the role of diet in developing a cariogenic biofilm in children under 2 years of age. How to cite this article Cornejo CF, Soken LJ, Salgado PA, et al. Detection of Streptococcus mutans and Streptococcus sobrinus and Their Association with Oral Microbiome Stressors in 6–18-month-old Infants. Int J Clin Pediatr Dent 2023;16(1):68-73.
In this study, comprehensive clinics (CC) are interpreted as a pragmatic projection of the complexity of health. The aim of the study was to analyze the characteristics of the CCs at different schools of dentistry in Argentina. Data were collected from document analysis and interviews with key respondents at 8 universities, classified according to the criteria of Bray and Thomas. Interviews were developed and analyzed according to discourse analysis. The convergences and divergences among curricula were established and analyzed statistically. Triangulation of results showed that: (a) Most curricula (7/8) included subjects called CC; (b) the permanence of CCs in the curricula was not stable, with changes recorded in different periods; and (c) in the so-called CCs, the complex clinical approach showed frequent displacements based on teacher values or competence. It was concluded that there are divergences between theory and practice, and that it would be recommendable to have more rigorous curricular design taking into account current trends regarding the complexity of health and its transfer to educational management.
Introduction The hepatitis C virus (HCV) infection is a health condition affecting 3% of the world population, which oral manifestations and associated factors interest both physicians and dentists. The aim of this work was to describe the dental treatment need and the impact of the perception of the oral component of health on the quality of life in HCV+ patients. Methodology Descriptive study on a convenience sample. 45 HCV+ patients (46±5 y.o.) completed the OHIP-14 questionnaire, which consists of 14 questions grouped in 7 domains (D1 functional limitation, D2 physical pain, D3 psychological discomfort, D4 physical disability, D5 psychological disability, D6 social disability and D7 general disability). The participants indicated their responses using a Likert-type frequency scale. The Community Caries Index of Treatment Need (CCITN) was determined for each patient. The proportion and CI95% of the social impact on the quality of life were calculated. The association between CCITN and the quality of life was assessed by Chi2 (p<0.05). Results The CCITN was 11 (8-14). The overall social impact was 38% (24-52%). The increasing order relationship of the impact on each of the domains was D1, D7, D6, D4, D5, D2, D3. A significant association between oral health-related quality of life and CCITN was observed (Chi2 = 7.57, p = 0.006), showing greater impairment of the quality of life as the treatment need increased. Conclusion The association between CCITN and quality of life becomes evident using OHIP-14 during dental appointments. The results suggest the need for comprehensive interventions during the provision of oral health care to HCV+ patients.
Candida dubliniensis (Cd) and Candida albicans (Ca) are the most frequently isolated yeasts in HIV+ patients. Some of the enzymes produced by these yeasts are considered virulence factors since they contribute to pathogenicity of Candida spp. The aim of the present study was to compare production of enzymes such as phospholipase (Ph), proteinase (P), and hemolysin (H) by Cd and Ca strains isolated from periodontal HIV-positive patients receiving and not receiving highly active antiretroviral therapy (HAART). Subgingival biofilm samples were obtained using paper points, and a sample of oral mucosa was taken using a swab. Phenotypic and molecular methods were used to isolate 39 strains of Candida, including 25 strains of Cd and 14 strains of Ca, obtained from 33 periodontal pocket samples and 6 oral mucosa samples collected from 15 HIV+ patients (8 receiving and 7 not receiving HAART). Malt egg-yolk agar, albumin agar and blood agar were used to evaluate pH, P and H production respectively. The strains were inoculated in duplicate and incubated at 37 ºC. Colony and halo diameters were measured. A greater proportion of Ca was observed in patients not receiving HAART, and a higher proportion of Cd was observed in those under HAART, Chi2 p< 0.001. Phospholipase production was observed in 92.9% percent of isolated Ca strains but in none of the isolated Cd strains. Proteinase production was high in Ca and Cd strains isolated from patients not receiving HAART. Hemolysin production was observed in all the studied strains, though it was significantly higher (p=0.04) in Ca and Cd strains isolated from patients not receiving HAART. To sum up, the proportion of Candida dubliniensis strains was highest in the subgingival biofilm of patients receiving HAART, and Cd strains were found to express fewer virulence factors than Ca strains.
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