Kandungan flavonoid dalam ekstrak daun tin (Ficus carica L.) memiliki aktiftas anti diabetes. Namun, ekstrak daun tin memiliki kelarutan dan absorpsi yang rendah. Penelitian ini bertujuan untuk mengetahui efektivitas liposom ekstrak daun tin sebagai antihiperglikemia terhadap kadar glukosa darah puasa ikan zebra. Ekstrak daun tin distandarisasi berdasarkan parameter nonspesifik kemudian diformulasikan ke dalam liposom. Selanjutnya dilakukan karakterisasi melalui pengamatan terbentuknya liposom secara visual, pembacaan ukuran partikel dengan Particle Size Analyzer (PSA), uji stabilitas, dan Transmission Electron Myroscope (TEM). Uji antihiperglikemia terdiri dari tiga kelompok dan dipilih secara acak. Kelompok kontrol negatif, positif, dan perlakuan diberi Aloksan pada hari pertama dan glukosa 1%. Pada hari ke-7, kelompok kontrol positif diberi Metformin, sedangkan kelompok perlakuan diberi liposom. Ikan zebra diperiksa kadar gula darah puasa dengan glucometer. Formula ke-5 dengan waktu ultrasonikasi selama 8 menit telah memenuhi kriteria sediaan liposom yang baik dengan karakteristik warna putih susu, ukuran partikel 116,17 ± 4,40nm; PI 0,439 ± 0,01Ð; zeta potensial -18,53 ± 0,41mV; stabil selama 24 jam; dengan morfologi bentuk bulat, memanjang, dan tidak beraturan. Liposom ekstrak daun tin terbukti mampu menurunkan kadar glukosa darah puasa ikan zebra sebesar 62,09%. Dapat disimpulkan bahwa liposom ekstrak daun tin berpotensi sebagai alternatif pengobatan antihiperglikemia.
This research aim to validation of a simple, rapid and accurate HPLC-UV method for the quantification of andrographolide isolated from Andrographis paniculata Ness in Self Nano Emulsifying Drug Delivery System (SNEDDS) formulation during the dissolution test. The assay was performed using a XTerra® MS C18 column (150 mm X 4.6 mm, five μm) with a mobile phase of methanol and water (70: 30), at 0.8 mL/min flow rate and UV detection of 229 nm. Simulation gastric fluid (SGF) and intestinal fluid (SIF) were prepared as dissolution medium. The validation parameter was conducted including the test on linearity, precision, accuracy, LOD, and LOQ. The result showed an excellent linearity with r = 0.999 and good selectivity for both medium dissolution. The method showed sufficient precision, with a relative standard deviation (RSD) smaller than % Horwitz. The accuracy reported as % recovery was found to be 102.61 and 101.17 % in each SGF and SIF dissolution medium. LOD and LOQ were found 0.46 and 1.40 in SGF medium, 0.87 and 2.64 in SIF medium. In conclusion, the HPLC method developed showed specificity and selectivity with linearity in the working range, good precision and accuracy and suitable for quantification andrographolide in SNEDDS formulation.
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