To cite this version:Alexandre Labergue, Valérie Deprédurand, Alain Delconte, Guillaume Castanet, Fabrice Lemoine. New insight into two-color LIF thermometry applied to temperature measurements of droplets. Experiments in Fluids, Springer Verlag (Germany), 2010, 49 (2), pp.547 -556. <10.1007/s00348-010-0828-x>. New insight into two-color LIF thermometry applied to temperature measurements of dropletsAbstract When laser-induced fluorescence of droplets is used for measurements such as droplet temperature, a new dependence of the droplet size on the spectral distribution of fluorescence has been highlighted. The two-color laserinduced fluorescence technique applied to droplet temperature measurement requires a single fluorescent tracer and two spectral bands of detection for which the temperature sensitivity is different. Generally, the ratio of the intensities measured on each of the spectral bands of detection is assumed to be only temperature dependent. However, droplet dependence on diameter is also likely to influence the intensities ratio. This study provides some illustrations of the phenomenon, first on sprays with different mean statistical diameters and secondly on single droplets, for two temperature-sensitive fluorescent tracers in their solvents: sulforhodamine B dissolved in water and pyrromethene 597-8C9 dissolved in n-decane.
To cite this version:Guillaume Castanet, Alain Delconte, Fabrice Lemoine, Loïc Mees, Gèrard Gréhan. Evaluation of temperature gradients within combusting droplets in linear stream using two colors laser-induced fluorescence. Experiments in Fluids, Springer Verlag (Germany), 2005, 39 (2), pp.431 -440. 10.1007/s00348-005-0931-6. hal-01570421 ORIGINALS Guillaume Castanet AE Alain Delconte AE Fabrice Lemoine Loı¨c Mees AE Ge`rard Gre´han Evaluation of temperature gradients within combusting droplets in linear stream using two colors laser-induced fluorescence Abstract The scope of this paper concerns the heating process of fuel droplets injected in a hot gaseous environment. The two colors laser-induced fluorescence technique allows measuring the temperature distribution within a droplet by scanning the droplet volume by a sufficiently small probe volume compared to the droplet volume itself. The temperature field is reconstructed using two approaches which have been compared. One is based on a geometrical optics model and the other is based on the 3D calculation of the internal excitation field within the droplet, using the generalized LorenzMie theory. Experimental results have been obtained on a combusting monodisperse ethanol droplet stream (diameter around 200 lm).
This paper presents a new implementation of an optical technique which aims to measure the temporal and spatial evolution of the liquid temperature of a high injection pressure Diesel spray. Measurements are performed using the two-colour laser-induced fluorescence technique in which a temperature-sensitive fluorescent tracer is added to the fuel (ISO 4113 normafluid in the present case) and all the parameters except the temperature (e.g. tracer concentration, incident laser excitation, droplet number density) are eliminated by processing the ratio of the fluorescence intensity measured on two spectral bands. Diesel sprays, issuing from a single-hole injector, are injected at high pressures ranging from 500 to 1500 bar at a frequency of 10 Hz. The signal acquisition is then triggered on the injection cycle to enable the phase-locked monitoring of the liquid phase temperature. Temperature maps of the spray are presented and the influence of the injection pressure on the droplet temperature can be finally inferred from these results.
Tissue engineering may constitute a promising alternative to current strategies in ligament repair, providing that suitable scaffolds and culture conditions are proposed. The objective of the present contribution is to present the design and instrumentation of a novel
OPEN ACCESSProcesses 2014, 2 168 multi-chamber tension-torsion bioreactor dedicated to ligament tissue engineering. A preliminary biological evaluation of a new braided scaffold within this bioreactor under dynamic loading is reported, starting with the development of a dedicated seeding protocol validated from static cultures. The results of these preliminary biological characterizations confirm that the present combination of scaffold, seeding protocol and bioreactor may enable us to head towards a suitable ligament tissue-engineered construct.
X-ray-induced photodynamic therapy is based on the energy transfer from a nanoscintillator to a photosensitizer molecule, whose activation leads to singlet oxygen and radical species generation, triggering cancer cells to cell death. Herein, we synthesized ultra-small nanoparticle chelated with Terbium (Tb) as a nanoscintillator and 5-(4-carboxyphenyl succinimide ester)-10,15,20-triphenyl porphyrin (P1) as a photosensitizer (AGuIX@Tb-P1). The synthesis was based on the AGuIX@ platform design. AGuIX@Tb-P1 was characterised for its photo-physical and physico-chemical properties. The effect of the nanoparticles was studied using human glioblastoma U-251 MG cells and was compared to treatment with AGuIX@ nanoparticles doped with Gadolinium (Gd) and P1 (AGuIX@Gd-P1). We demonstrated that the AGuIX@Tb-P1 design was consistent with X-ray photon energy transfer from Terbium to P1. Both nanoparticles had similar dark cytotoxicity and they were absorbed in a similar rate within the cells. Pre-treated cells exposure to X-rays was related to reactive species production. Using clonogenic assays, establishment of survival curves allowed discrimination of the impact of radiation treatment from X-ray-induced photodynamic effect. We showed that cell growth arrest was increased (35%-increase) when cells were treated with AGuIX@Tb-P1 compared to the nanoparticle doped with Gd.
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