Problem statement: It was intended to diagnose and treat the disorders of female reproductive system where they are exposed to environmentally toxic pesticides and estrogenic compounds like bisphenol A which are the main reason for the infertility problems. Approach: Human saliva contains arrays of proteins that have distinct biological functions. Results: Salivary hormones concentrations of luteinising hormone, follicle stimulating hormone, estrogen as well as estrone-1-glucuronide, were assayed in 20 normal menstruating women who were among the reproductive phases like preovulatory phase (6-12 days); Ovulatory phase (13-14 days) and Postovulatory phase (15-26 days). It was intended to diagnose and treat the disorders of female reproductive system where they are exposed to environmentally toxic pesticides and estrogenic compounds like bisphenol A which are the main reason for the infertility problems. In this context, the detection of fertile period suggests that the mean value of luteinising hormone (56.58±12.96 mIU L −1 ) indicates the surge during the time of ovulation.Identifying the period of ovulation would be the easiest way to detect other constituents like follicle stimulating hormone, estrogen and its conjugate like estrone-1-glucuronide are extensively high (p<0.05) was observed. Conclusion: Our results indicated that the salivary hormones like estrogen and its conjugate indicates decrease in ovulatory and postovulatory phase. Further, the environmental toxic materials can also cause problems like infertility. The conclusion is that any physiological effects of estrogen from drinking water will be undetectable in people. The results from hormones suggested that they may be considered in the diagnosis and treatment of ovulation using saliva as the noninvasive specimen.
Lipases are glycerol ester hydrolases that catalyze the hydrolysis of triglycerides to free fatty acids and glycerol. Oil spilled soil samples collected from Dharmapuri and Salem districts were screened for a novel lipase producing bacteria by screening in Tributyrin agar plate and Rhodamine olive oil agar and lipase screening liquid medium. Among the two hundred isolates thirty two isolates exhibited high lipolytic activity on TBA plate assay and twenty isolate exhibited high lipolytic activity on ROA plate assay The isolate BLP 141 produces lipase more than 12 U/ml in the further lipase screening liquid medium was selected as a novel lipase producing bacteria and identified by morphological and biochemical characteristics as Pseudomonas. The 16S rRNA gene amplified by bacterial universal primer 27F and 1492R yielded 1478 bp was analysed by BLAST confirmed it as Pseudomonas gessardii and was submitted in GenBank with accession number KJ547711.
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