The encapsulin nanocompartment from Rhodococcus erythropolis N771 (Reencapsulin) was expressed and purified in wild-type and C-terminally His-tagged forms. Negative-stained transmission electron microscopy, fieldflow fractionation combined with multi-angle light scattering and dynamic light scattering analyses showed that 60 Reencapsulin monomers were assembled as a spherical particle with a diameter of 28 nm. Heterogeneous guest proteins such as EGFP and firefly luciferase were packaged into the internal cavity of the Reencapsulin nanocompartment by fusing the C-terminal 37-amino-acid sequence of the R. erythropolis N771 DypB peroxidase to the C-terminus. Reencapsulin has the potential to package target proteins in its internal cavity and/or display them on its external surface, making it a feasible carrier for nanotechnology applications.
Acyclic partial scan design is an efficient DFT method. This paper presents a scheduling method for reducing the number of scan registers for acyclic structure. In order to estimate the number of scan registers during scheduling, we propose provisional binding of operational units, and show a force-directed scheduling algorithm with the provisional binding. Experimental results show that the number of scan registers in the resulting RTL datapaths can be reduced by our method combined with the binding algorithm for acyclic partial scan.
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