Since there is no available serological methods to detect antibodies to ferret coronavirus (FRCoV), an
enzyme-linked immunosorbent assay (ELISA) using recombinant partial nucleocapsid (N) proteins of the ferret
coronavirus (FRCoV) Yamaguchi-1 strain was developed to establish a serological method for detection of FRCoV
infection. Many serum samples collected from ferrets recognized both a.a. 1–179 and a.a. 180–374 of the N
protein, but two serum samples did not a.a. 180–374 of the N protein. This different reactivity was also
confirmed by immunoblot analysis using the serum from the ferret.Therefore, the a.a. 1–179 of the N protein
was used as an ELISA antigen. Serological test was carried out using sera or plasma of ferrets in Japan.
Surprisingly, 89% ferrets in Japan had been infected with FRCoV. These results indicated that our established
ELISA using a.a. 1–179 of the N protein is useful for detection of antibody to FRCoV for diagnosis and
seroepidemiology of FRCoV infection.
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