Blood collection and component preparation have been performed in integrally connected multiple plastic containers made with a new plastic. This polyvinylchloride (PVC) container plasticized with butyryl-n-trihexyl-citrate (BTHC) is a new material for blood storage; it contains no di(2-ethylhexyl)phthalate (DEHP). After removal of plasma and buffy coat, the red cells were suspended in saline-adenine-glucose-mannitol (SAGM) medium. After 42-day refrigerator storage, the total adenine nucleotide concentration remained the same as the initial concentration in the red cells, whereas ATP levels had decreased to 61 percent of the initial value. The 2,3 DPG concentration was 62 percent of normal on Day 7 and 21 percent on Day 14. Glucose consumption, lactate production, potassium leakage from red cells, and pH levels were similar to those found after storage in DEHP-plasticized containers under the same conditions. After 42 days, hemolysis levels were 0.56 +/- 0.21 percent and 0.42 +/- 0.17 percent in two series of units mixed weekly and 0.70 +/- 0.27 percent in units stored unmixed. Although even higher levels of hemolysis were observed in the units stored unmixed and used for 24-hour posttransfusion survival, the autologous red cell recovery results were excellent (83.2 +/- 5.1%, n = 8). BTHC-plasticized PVC is found to be a suitable material for 42-day storage of red cells in SAGM solution.
Red cells collected in CPD and suspended in SAGM medium were stored in plastic (PVC) containers for
42 days at +4°C. Comparison was made between aerobic storage (normal air exposure) and anaerobic storage
(exposure to nitrogen gas). The air-exposed units showed a strong increase in pO(2) and oxygen saturation as a result of
oxygen penetration into the bags from outside. This resulted in a decrease in ATP and adenylate energy charge, a
slower metabolization of adenine and hypoxanthine to AMP and IMP, respectively, and a faster decrease in red cell
fluidity. To explain the findings it is concluded that aerobic storage causes an increased need of high-energy
phosphate groups, possibly used for replacement of the phospholipid membrane bilayer or in repair of phosphate
bonds in the cytoskeleton. It is further proposed that a slight formation of hydrogen peroxide from free oxygen
radicals moderately increases the oxidation of reduced (GSH) to oxidized (GSSG) glutathione and slightly enhances
the need for reduced nicotinamide-adenine dinucleotides mainly provided by increased flux through the pentose
phosphate shunt.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.