Storage of saline‐adenine‐glucose‐mannitol‐suspended red cells in a new plastic container: polyvinylchloride plasticized with butyryl‐n‐ trihexyl‐citrate

Högman, Claes F.; Eriksson, Lars; Ericson, Åke; Reppucci, A.J.

doi:10.1046/j.1537-2995.1991.31191096180.x

Cited by 67 publications

(44 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Many parameters indicated that, overall, RBCs stored in BTHC-plasticized bags showed poorer membrane preservation capacity than those in DINCH- or DEHP-plasticized bags. A previous study from 1991, found that overall RBC quality in DEHP- and BTHC-plasticized PVC was generally comparable [24]. Our results, focused on events at the RBC membrane, would suggest differently.…”

Section: Discussioncontrasting

confidence: 44%

Blood Bag Plasticizers Influence Red Blood Cell Vesiculation Rate without Altering the Lipid Composition of the Vesicles

Bicalho

Serrano

Pereira

et al. 2015

Transfus Med Hemother

View full text Add to dashboard Cite

Background: Polyvinyl chloride (PVC) plasticized with di(2-ethylhexyl) phthalate (DEHP) is commonly used for blood collection and storage. DEHP has protective effects on RBC membranes, but is also a toxin. Methods: A paired study was conducted to investigate the influence of DEHP and two alternative plasticizers, 1,2-cyclohexane-dicarboxylic acid diisononyl ester (DINCH) and n-butyryl-tri-n-hexyl citrate (BTHC), on the preservation of RBCs stored for 42 days in PVC pediatric bags. The RBC membrane was evaluated for supernatant hemoglobin (Hb), release of extracellular microvesicles (EVs), osmotic fragility, deformability, and lipid composition. Results: In BTHC-plasticized bags, the supernatant Hb increase during storage was 2 times greater than in DINCH- and DEHP-plasticized bags. By day 21, EV concentrations had doubled from day-5 levels in DINCH- and DEHP-, and trebled in BTHC-plasticized bags. RBC mean cell volumes were greater in BTHC- than in DINCH- or DEHP-plasticized bags (p < 0.001). Osmotic fragility differed significantly among plasticizers (p < 0.01). After day 21, RBC deformability decreased in all, but to a greater extent in the bags with BTHC. Phospholipid composition of RBCs and EVs was not different among plasticizers. Conclusion: Membrane stabilization capacity differed among the plasticizers. RBC in BTHC bags stored more poorly, while DEHP and DINCH bags offered better protection against vesiculation, osmotic stress, and Hb loss.

show abstract

Section: Discussioncontrasting

confidence: 44%

Blood Bag Plasticizers Influence Red Blood Cell Vesiculation Rate without Altering the Lipid Composition of the Vesicles

Bicalho

Serrano

Pereira

et al. 2015

Transfus Med Hemother

View full text Add to dashboard Cite

show abstract

“…During a period of 42 days of storage, free hemoglobin/methemoglobin level reaches to 200 mg/ dl [26]. According to the American association of blood banks (AABB) blood or blood products with 1% hemolysis is safe for transfusion, if it provides 75% RBCs in the next 24 h [27].…”

Section: Discussionmentioning

confidence: 99%

Extracellular Methemoglobin Mediated Early ROS Spike Triggers Osmotic Fragility and RBC Destruction: An Insight into the Enhanced Hemolysis During Malaria

Balaji

Trivedi

2011

Ind J Clin Biochem

View full text Add to dashboard Cite

Malaria infection is known to cause severe hemolysis due to production of abnormal RBCs and enhanced RBC destruction through apoptosis. Infected RBC lysis exposes uninfected RBC to the large amount of pro-oxidant molecules such as methemoglobin. Methemoglobin (MetHb) exposure dose dependently makes RBCs susceptible to osmotic stress and causes hemolysis. MetHb mediated oxidative stress in RBC correlated well with osmotic fragility and hemolysis. Interestingly, a reactive oxygen species (ROS) spike at 15 min was responsible for the observed effects on RBC cells. Two natural antioxidants N-acetyl cysteine and mannitol protected the RBC from MetHb-mediated defects, which clearly indicated involvement of oxidative stress in the process. MetHb due to its pseudo-peroxidase activity produces ROS in the external microenvironment. Therefore, classical peroxidase inhibitors were tested to probe peroxidase activity mediated ROS production with defects in RBCs. Clotrimazole (CLT), which irreversibly inactivates the MetHb (CLTMetHb) and abolishes peroxidase activity, did not produce significant ROS outside RBC and was inefficient to cause osmotic fragility and hemolysis. Hence, initiating a chain reaction, MetHb released from ruptured RBC produces significant ROS in the external microenvironment to make RBC membrane leaky and enhanced hemolysis. Together data presented in the current work explored the role of MetHb in accelerated humorless during malaria which could be responsible for severe outcomes of pathological disorders.

show abstract

“…2,3-BPG was analysed by an enzymatic assay [13] using a test kit (Boehringer-Mannheim, Germany) and Sigma metabolite control (Sigma Diagnostics, USA) in a Cobas Mira S (Roche Diagnostics, Hoffmann-LaRoche, Basel, Switzerland).…”

Section: Methodsmentioning

confidence: 99%

Improved Blood Preservation with 0.5CPD Erythro-Sol. Coagulation Factor VIII Activity and Erythrocyte Quality after Delayed Separation of Blood

et al. 1998

View full text Add to dashboard Cite

Background and objectives: Delay between blood collection and the separation of its components may result in lowered yield of factor VIII (FVIII) and loss of 2,3-biphosphoglycerate (2,3-BPG). This study was to see whether the use of 0.5 CPD resulted in better preservation of FVIII and maintenance of 2,3-BPG. Materials and methods: 55 units of blood were collected in 0.5CPD and 48 in CPD SAG-M. Ten of the collections were paired, so that the same donors were bled in a single session partly in an 0.5CPD system and partly in CPD SAG-M. After collection, the blood was promptly cooled to 20 °C and stored at that temperature for up to 24 h. Results: Preservation of FVIII activity was significantly better in 0.5CPD compared with CPD. The content of von Willebrand factor was stable in the anticoagulant solutions for 24 h at that temperature. Plasma separated from both media had low levels of prothrombin fragment 1+ 2 and complement activation. Paired collections substantiated previous reports that red cell storage is significantly improved in 0.5CPD compared with CPD SAG-M with respect to 2,3-BPG and haemolysis. Conclusions: Red cell metabolism and oxygen-releasing capacity are kept at acceptable levels in 0.5CPD blood for 24 h at 20 °C before component separation. The concentration of red cell 2,3-BPG remained at normal or slightly subnormal levels during further storage in 0.5CPD at 4 °C for 2–4 weeks before gradual decay to an average of 39% at 48 days.

show abstract

Storage of saline‐adenine‐glucose‐mannitol‐suspended red cells in a new plastic container: polyvinylchloride plasticized with butyryl‐n‐ trihexyl‐citrate

Cited by 67 publications

References 16 publications

Blood Bag Plasticizers Influence Red Blood Cell Vesiculation Rate without Altering the Lipid Composition of the Vesicles

Blood Bag Plasticizers Influence Red Blood Cell Vesiculation Rate without Altering the Lipid Composition of the Vesicles

Extracellular Methemoglobin Mediated Early ROS Spike Triggers Osmotic Fragility and RBC Destruction: An Insight into the Enhanced Hemolysis During Malaria

Improved Blood Preservation with 0.5CPD Erythro-Sol. Coagulation Factor VIII Activity and Erythrocyte Quality after Delayed Separation of Blood

Contact Info

Product

Resources

About