The Centrifugal liquid Membrane (CLM) method, which provides an ultra-thin two-phase liquid membrane system in a rotating glass cell, was successfully applied to Green Synthesis from Polyscias scutellaria (PS) capped gold nanoparticles (AuNPs-PS) using a Mangkokan leaf (Polyscias scutellaria) extract as a reducing agent and stabilizer in the hexane-water system. PS extract in hexane fraction as the organic phase has a UV absorption spectrum at the maximum wavelength, λmax of 220 nm, while the precursor of HAuCl4 solution as an aqueous phase has an λmax of 214 nm. Investigation of AuNPs-PS formation was carried out at various concentrations of Mangkokan leaf extract concentration; i.e., 0.001 0.003; 0.005; 0.007 and 0.009%, while the reaction was carried out at various rotational speeds of 5,000-9,000 rpm. The formation and stability of AuNPs-PS were observed from the phenomenon of surface plasmon resonance (SPR) and absorbance changes as measured by a UV-Vis spectrophotometer. The results of measurements using CLM-Spectrophotometry shows the formation of AuNPs-PS in the hexane-water system at λmax of 534 nm.
THE POTENTIAL OF ACTIVE COMPOUNDS Polyscias Scutellaria AS INHIBITORS IN CERVICAL CANCER WITH VIRTUAL SCREENING APPROACHCervical cancer is a gynecologic disease that has a high enough malignancy that affects women. In cervical cancer, it is known that the overexpression of Bcl-2 protein is 14.3% in non-invasive cervical carcinoma and 65.2% in invasive cervical carcinoma. The Bcl-2 protein is an important regulator of growth and differentiation pathways. Bcl-2 has anti-apoptotic activity but permeabilization occurs through the mitochondrial pathway, triggering the release of cytochrome c, which then interacts with Apaf-1 for caspase-9 activation and apoptosis occurs. This study aims to determine the mechanism of inhibition of overexpression of Bcl-2 protein by bioactive compounds from the Polyscias scutellaria plant with a virtual screening approach through in silico studies. The in silico study was carried out with the stages of tracking molecular targets, preparation, optimization, simulation and analysis of docking results. The results of the docking analysis showed that the bioactive compounds of the mangkokan plant provided inhibitory activity with gibbs energy values of -6.07, -5.18, -5.43 and -6.02, respectively. Thus the bioactive compounds from the mangkokan plant have potential as Bcl-2 inhibitors in cervical cancer.
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