Exposure of rabbit bucks to summer heat stress reduces their homeostasis and semen quality leading to a temporal subfertility. The potentiality of ethanolic extract of Moringa oleifera leaves (M. oleifera ethanolic extract (MLEE)) to reduce negative impacts of heat stress on physiological and semen quality traits was investigated. A total of 28 adult V-line rabbit bucks were randomly distributed among four experimental groups of seven rabbits each. The first group received water (placebo) and served as a control (M0). The other three groups were given orally MLEE at levels of 50 (M50), 100 (M100) and 150 (M150) mg/kg BW every other day for 12 consecutive weeks during the summer season. Chemical constituents of MLEE were detected by gas chromatography/MS. During the experimental period, ambient temperature and relative humidity were recorded daily and were used to estimate temperature and humidity index. Feed intake, BW, rectal temperature were recorded and blood serum biochemical attributes were determined. Semen samples were collected weekly and were analyzed for semen quality traits. Results showed that MLEE contained high percentages of long-chain fatty acids and antioxidant agents. Feed intake and BW were not affected significantly by the treatment, however rectal temperature was decreased significantly by 0.42°C, 0.24°C and 0.40°C in the M50, M100 and M150 groups, respectively, compared with the M0 group. Treatment with 50 mg/kg BW increased concentration of serum albumin (115%; P<0.05), total antioxidant capacity (132%; P<0.05) and testosterone (160%; P=0.098) as well as seminal plasma initial fructose (127%; P=0.092) compared with the control group. Compared with the control, MLEE supplementation with 50, 100 and 150 mg/kg BW increased significantly sperm concentration by 118%, 151% and 158%, sperm progressive motility by 117%, 120% and 118%, sperm viability by 129%, 137% and 127%, sperm normal morphology by 114%, 113% and 114%, intact acrosome sperm by 109% (on average) and sperm with integrated cell membrane by 109%, 123% and 114%, respectively. In conclusion, MLEE supplementation at a level of 50 mg/kg BW could be effectively used to improve heat tolerance, oxidative status and semen quality of rabbit bucks during summer season.

This study investigated the effect of Moringa leaf ethanolic extract (MLEE) on heat-tolerance variables and the reproductive performance of rabbit does bred under hot climate conditions. Additionally, the effect of nanoencapsulation technology on the biological efficiency of MLEE was considered. A total of 56 rabbit does were randomly divided into four experimental groups and treated with 50 mg/kg body weight (BW) nonencapsulated MLEE, 25 or 10 mg/kg BW nanoencapsulated MLEE, or not treated (Control, C). The treatments continued for 50 days, including mating and pregnancy times. Physiological and hematochemical variables, hormonal profiles, and reproductive performance (kindling rate and litter characteristics) were determined. The active components of MLEE were identified. The results indicated that MLEE has 30 active components. All MLEE-based treatments reduced heat-stress-related indicators, such as rectal temperatures, respiratory rates and heart rate; improved hematochemical attributes, redox status, and hormones (progesterone and prolactin); and increased the total litter size, the kindling rate, litter size at birth and litter weight at birth. Adding MLEE can alleviate the negative impacts of heat stress by improving metabolism, redox status, and hormonal balance during pregnancy. These effects were seen whether MLLE was in free or encapsulated forms. However, the use of nanoencapsulated MLEE allowed 80% reduction (10 mg/kg BW) in the optimal dose (50 mg/kg BW) without affecting the efficiency of the treatment. These results support the importance of nanoencapsulation technology in improving the bioavailability of active components when they are orally administered.

The effects of free and nano-encapsulated ME supplementations on the metabolism, immunity, milk production and composition, and reproductive performance of rabbit does during premating, mating, pregnancy, and lactation were investigated. Multiparous rabbit does (n = 26 per group) received 50 mg of free ME (FME) daily, 25 mg of nano-encapsulated ME (HNME), or 10 mg of nano-encapsulated ME (LNME) per kilogram of body weight or were not supplemented (C) during a whole reproductive cycle. The ME contained 30 fatty acids with 54.27% total unsaturated fatty acids (USFAs). The fatty acid encapsulation efficiency of alginate nanoparticles was 70.46%. Compared with the C group, rabbits in all ME treatments had significantly increased body weight, feed intake, and glucose concentration and significantly decreased non-esterified free fatty acids and β-hydroxybutyrate concentrations. Rabbits supplemented with ME also had significantly increased white blood cell counts, phagocytic activity, lysozyme activity, and immunoglobulin G and decreased interleukin-1β concentrations. Moreover, ME supplementation significantly increased the concentrations of colostrum immunoglobulins, milk yield and energy content, and milk USFAs (omega-3 and 6). Rabbit does in the ME treatments had significantly higher conception and parturition rates and better litter characteristics than the C rabbit does. These results demonstrate the positive role of ME fatty acids on the health status and productive and reproductive performance of rabbit does at different physiological stages. Compared with the FME treatment, these parameters were further improved in rabbits that received nano-encapsulated ME at lower doses, illustrating how nano-encapsulation technology improves the bioavailability of ME.

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orty newly born male Holstein calves were distributed randomly into four treatments (n=10) to receive no additive (control), Se (2.3mg/head/d), Humic acid (HA, 15 g/head/d), HA + Se (15 g HA+2.3 mg Se/head/d) to evaluate the effect of treatments on calves performance, serum IgG concentration and some blood parameters. Calves received their respective treatments for four consecutive days after birth as treatments were mixed with colostrum and blood samples were taken at 12, 24, 48 and 72 hrs. after birth and biweekly until 75 th day of life. Treatment had no effect on ADG, feed intake or weaning weight. Se and or HA had no effect on IgG concentration at 48 hrs. Se treatment increased (P<0.05) serum TP and HA reduced (P<0.05) serum TP compared to control and HA+Se. All treatments (Se, HA, HA+Se) increased (P<0.05) serum Alb concentration compared to control. Se supplementation increased (P<0.05) Glob concentration, however HA+Se reduced (P<0.05) Glob. Therefore HA+Se and HA had the higher A/G ratio compared to Se and control. Treatments had no effect on serum glucose (G). Se increased (P<0.05) G whereas, HA and HA+Se reduced (P<0.05) G significantly compared to control. Treatments with HA and HA+Se decreased (P<0.05) serum urea compared to control, whereas HA and increased (P<0.05) serum cholesterol but HA+Se group had the least cholesterol concentrations. All treatments reduced (P<0.05) serum TG compared to control. Se supplementation increased TP, Glob and G contrary to HA effect. More research is needed to evaluate the effect of Se and HA in animal health and performance.