Formaldehyde and benzo(α)pyrene are compounds that harmful for health. Misapplication of this compound has an impact in the form of organ damage in the body. This study aims to determine t he impact of the treatment of the combined exposure of formaldehyde and benzo(α)pyrene to cell necrosis in the liver of mice (Mus musculus). Treatment of formaldehyde dose of 25 mg/kg BW to mice was given orally every day for 60 days. Treatment of benzo(α)pyrene via ip at a dose of 250 mg/kg BW were given after 30 days of incubation with four times injection with one day interval. Liver organ histological preparations were made through the HE staining and were observed by BX51 microcopy. The data obtained that is the percentage of cells necrosis and necrotic foci. This research used Completely Randomized Design (CRD) with 95% confidence interval. Liver organ preparations observations indicate that the percentage of necrosis in the untreated control, benzo(α)pyrene 250 mg/kg BW, formaldehyde 25 mg/kg BW, combination of formaldehyde 25 mg/kg BW with benzo(α)pyrene in a row that is equal to 14.43% ± 0.91; 26.05% ± 3.75; 49.38% ± 2.66; 51.86 ± 1.73. The mean of necrotic foci in liver organ formed in the untreatment control, benzo(α)pyrene 250 mg/kg BW, Formaldehyde 25 mg/kg BW, and the combination of formaldehyde 25 mg/kg BW with benzo(α)pyrene in a row, equal to 1.3 ± 0,07; 1.63 ± 0.61; 2 ± 0.51, and 3.4 ± 0.76. This suggests that the combined treatment had the highest level of toxicity compared with other treatments.
Coral reef ecosystem is the most threatened ecosystem among marine ecosystem in the world due to the combination of anthropogenic and natural disturbances. More research is needed to be monitored and assess coral reef ecosystems, which will be used to find understanding of the ecological integrity and further improvement of the protection strategy in the future. This research was aimed to know the diversity of coral reef diversity at Gili Genting Island, Sumenep Madura and evaluate the condition of coral reef ecosystem based on cover the percentage. Line intercept method was used to understand coral reef diversity and its condition in the desired observation station. The result revealed that 9 families, 22 genera and 45 species of coral have been successfully found, in which Acropora is the most common genus found in in Gili Genting Island. The cover percentage analysis also indicated that the condition of coral reef ecosystem in Gili Genting Island could be classified to very bad category where the total average of cover percentage is 12.55%. These findings provide the preliminary information about the condition of coral reef ecosystem in small island that might be useful for the future integrated management based on ecological perspective.
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