Psoriasis is a common inflammatory skin disease and dendritic cells (DCs) play crucial role in the development of skin inflammation. Although the characteristics of skin DCs in psoriasis are well defined, less is known about their peripheral blood precursors. Our aim was to characterize the phenotypic features as well as the cytokine and chemokine production of CD1c myeloid DCs (mDCs) and plasmacytoid DCs (pDCs) in the blood samples of psoriatic patients. Blood DCs were isolated by using a magnetic separation kit, and their intracytoplasmic cytokine production and CD83/CD86 maturation/activation marker expression were investigated by 8-colour flow cytometry. In CD1c mDCs the intracellular productions of Th1, Th2, Th17, Th22 and Treg polarizing cytokines were examined simultaneously, whereas in pDCs the amounts of IFNα as well as IL-12, IL-23 and IL-6 were investigated. The chemokine production of both DC populations was investigated by flow-cytometry and ELISA. According to our results psoriatic CD1c mDCs were in a premature state since their CD83/CD86 maturation/activation marker expression, IL-12 cytokine, CXCL9 and CCL20 chemokine production was significantly higher compared to control cells. On the other hand, blood pDCs neither produced any of the investigated cytokines and chemokines nor expressed CD83/CD86 maturation/activation markers. Our results indicate that in psoriasis not only skin but also blood mDCs perform Th1 polarizing and Th1/Th17 recruiting capacity, while pDCs function only in the skin milieu.

The increase in the prevalence and age of seborrhea, its significant impact on the psycho-emotional sphere, social status and social adaptation of patients determines the relevance of further study of the causes of dermatosis in the key of constitutional psychodermatology, namely – the study of relationships between oily skin and emotional impact of oily skin with anthropometric indicators. The aim of the study was to analyze the correlations between Oily Skin Self Assessment Scale (OSSAS) and Oily Skin Impact Scale (OSIS) in men and women with seborrheic dermatitis with body structure and size indicators. A survey of 40 men and 40 young women with generalized fatty seborrheic dermatitis done. The OSSAS and OSIS scoring system was used to assess the severity of oily skin and the emotional impact of oily skin in seborrheic dermatitis. Anthropometric survey was carried out according to the scheme of Bunak V. V. (1941). The mathematical scheme of Carter J. and Heath B. (1990) was used to evaluate the somatotype. Matiegka J. (1921) formulas were used to calculate body weight components. In addition, the muscle component of body weight was assessed by the American Nutrition Institute. Correlation analysis was performed in the license package "Statistica 6.0" using the non-parametric Spearman's method. As a result of the conducted researches multiple reliable and moderate unreliable correlations of OSSAS or OSIS with anthropo-somatotypological indicators of men and women of patients with generalized fatty form of seborrheic dermatitis of mild and severe course were established. The practical lack of similar correlations between OSIS and anthropo-somatotypological parameters in men and women with severe seborrheic dermatitis is noteworthy. Quantitative analysis of reliable and moderate unreliable correlations of OSSAS or OSIS with anthropo-somatotypological parameters in men and women with seborrheic dermatitis of mild and severe course showed that the vast majority of such correlations are inverse, and unreliable average correlations are often observed (except for OSSAS correlations with anthropo-somatotypological parameters in women with mild disease). It was also found that regardless of sex, most of the reliable or moderately unreliable correlations between OSSAS or OSIS and anthropo-somatotypological indicators are observed in the mild course of the disease. The obtained results of the correlation analysis expand the current understanding of the risk criteria and unfavorable prognosis of seborrheic dermatitis.

Significant progress has been made in the diagnosis of seborrheic dermatitis. It is based on the anamnesis and clinical picture of skin lesions. Carrying out a detailed analysis of the structure and size of the body in combination with clinical and instrumental studies allows us to further make a more reliable prognosis of complications of this disease and improve the results of treatment of such patients. The aim of the study was to establish and analyze the features of the skinfold thickness in Ukrainian men and women with seborrheic dermatitis of varying severity. Skinfold thickness (SFT) was determined in 40 men and 40 women (aged 25 to 44 years) with generalized fatty seborrheic dermatitis (mild and severe). The control group consisted of SFT values of practically healthy men (n=82) and women (n=154) of the same age group from the database of the research center National Pirogov Memorial Medical University, Vinnytsya. Statistical processing of SFT indicators was performed in the license package “Statistica 6.0” using non-parametric evaluation methods. Compared with practically healthy men, patients with mild and severe seborrheic dermatitis had lower SFT values on the posterior (by 49.7 % and 46.5 %) and anterior (by 41.9 % and 46.4 %) surfaces of the shoulder and chest (by 28.9 % and 27.9 %), on the thigh (47.3 % and 38.3 %), on the forearm (only compared to severe severity by 18.5 %), at the lower angle of the shoulder blade (only compared to mild severity by 3.5 %) and on the shin (only compared to severe severity by 15.9 %), as well as higher values of SFT on the side (by 36.7 % and 51.6 %); and in women patients of varying severity– also lower values of SFT on the posterior surface of the shoulder (by 51.0 % and 43.6 %), on the anterior surface of the shoulder (by 46.6 % and 31.0 %), on the chest (by 31.3 % and 18.9 %), on thighs (by 47.4 % and 38.9 %) and on the shin (only compared to the mild degree by 10.2 %), as well as higher values of SFT on the side (by 37.0 % and 44.6 %). Among men or women with seborrheic dermatitis of varying severity, only higher values were found in women with severe SFT on the anterior surface of the shoulder (by 22.6 %), and in men with severe severity – higher values of SFT on the thigh (by 14.6 %). Manifestations of sexual dimorphism of SFT among patients with seborrheic dermatitis of varying severity were found only between men and women with severe disease, namely, higher values of SFT in women on the front shoulder surface (by 28.6 %), forearm (by 16.0 %) and on the shin (by 26.3 %).

In order to suppress self-reactive T cells that escape from central tolerance, additional mechanism in peripheries, so-called peripheral tolerance, is essential; however this mechanism is not fully elucidated. To investigate this issue, here we generated a novel murine transgenic line that expressed membrane-bound ovalbumin (mOVA) under the control of human involucrin (Ivl) promoter; Ivl-mOVA mice. Within one week after the transfer of CD8 + T cells from OT-I mice (OT-I T cells), GVHD-like cutaneous and mucosal eruptions developed. Histological analysis revealed massive infiltration of OT-I T cells to the epidermis. Intriguingly, most (>95%) of the transferred OT-I T cells were deleted in lymph nodes within 24 h, and subsequent extensive expansion of OT-I T cells was observed 48 h post transfer. This expansion was observed not only in skin-draining lymph nodes but also in mesenteric lymph nodes, spleen, and bone marrow. We used bone-marrow chimeric mice reconstituted with b2-microglobulin-deficient mice, which lack functional MHC class I, to identify the responsible cell subset for initial deletion of OT-I T cells. OT-I T cell deletion was observed even in these chimeric mice. This result indicates the essential role of radio-resistant cells, suggestive of stromal cells, in secondary lymphoid organs. Taken together, these results suggest that tissue-specific self-antigens presented by radio-resistant lymph node stromal cells control peripheral tolerance and development of GVHD-like skin lesion. 365Myeloid but not plasmacytoid blood DCs possess Th1 polarizing and Th1/Th17 recruiting capacity in psoriasis A Psoriasis is a common inflammatory skin disease and dendritic cells (DCs) play a crucial role in the development of skin inflammation. Although the characteristics of skin DCs in psoriasis are well defined, less is known about their peripheral blood precursors. Our aim was to characterize the phenotypic features as well as the cytokine and chemokine production of CD1c+ myeloid DCs (mDCs) and plasmacytoid DCs (pDCs) in the blood samples of psoriatic patients. Blood DCs were isolated by using a magnetic separation kit, and their intracytoplasmic cytokine production and CD83/CD86 maturation/activation marker expression were investigated by 8-colour flow cytometry. In CD1c+ mDCs, the intracellular productions of Th1, Th2, Th17, Th22 and Treg polarizing cytokines were examined simultaneously, whereas in pDCs the amounts of IFNa, as well as IL-12, IL-23, and IL-6, were investigated. The chemokine production of both DC populations was investigated by flow cytometry and ELISA. According to our results, psoriatic CD1c+ mDCs were in a premature state since their CD83/CD86 maturation/activation marker expression, IL-12 cytokine, CXCL9 and CCL20 chemokine production was significantly higher compared to control cells. On the other hand, blood pDCs neither produced any of the investigated cytokines and chemokines nor expressed CD83/CD86 maturation/activation markers. Our results indicate that in psoriasis not only skin but al...

Human CD83 is a type I transmembrane glycoprotein, primarily expressed on mature dendritic cells (DCs), but also on subsets of activated Band T cells. Increasing evidence suggests that while in vitro expression of membrane-bound CD83 (mCD83) on mature DC possesses stimulatory effects on immune response, a soluble form of CD83 (sCD83) has inhibitory effects. In this study, we used a model of hapten-induced skin inflammation, i.e allergic contact dermatitis (ACD), to gain further insight on the immuno-regulatory properties of sCD83. Intraperitoneal injection of sCD83 prior to skin sensitization with the potent chemical sensitizer 2,4-dinitrofluorobenzene (DNFB) significantly reduced the development of experimental ACD. Impaired allergic reaction was the result of a sequential inhibition of innate immune response, skin DC migration and T cell priming. Crucially, no inhibition of skin inflammation was recorded in animals deficient in B cells (Rag2-/-or mMT mice), in IL-10-/-mice and in CD83-conditionally deleted B cell mice, suggesting that B cells are central to the anti-inflammatory effects of this sCD83. Further study of the role of sCD83 as an inhibitor of ACD could yield valuable insights into the pathophysiology of inflammatory skin diseases as well as aiding the development of potential therapeutic strategies.

Annotation. Despite the prevalence of seborrheic dermatitis (SD), especially among young people, little is known about its clinical and anamnestic features in the population of Ukraine. To fill this gap, it is necessary to conduct a study of reliable data obtained from the domestic sample of working age. The aim of the study is to find the clinical and anamnestic features of men and women with generalized fatty form of seborrheic dermatitis of varying severity. A survey of 40 men and 40 young women (25-44 years according to the age periodization of the WHO, 2015) in patients with generalized fatty seborrheic dermatitis was conducted on the basis of the Department of Skin and Venereal Diseases with a postgraduate course in National Pirogov Memorial Medical University, Vinnytsya and the Military Medical Clinical Center of the Central Region. Retrospective analysis included assessment of passport, anamnestic, clinical data. Diagnosis of SD was established on the basis of complaints of the subject, life history and illness, examination of the face, scalp, torso and extremities with the assessment of subjective and objective signs of the disease. Statistical analysis was performed using the license package “Statistica 6.0”. It was found that the severity of SD increased with increasing length of service and depended on the type of professional activity. Stress, temperature, humidity, smoking, heredity caused the manifestation or exacerbation of dermatosis. Acne and rosacea often accompanied severe SD. Itching and oiliness of the skin, rash on the head and face, erythematous spots and plaques, the predominance of red dermographism were observed in both types of flow, and the feeling of dirt, localization of pathological elements on the body, sebaceous scales – only in severe. Thus, the data obtained in our study can form the basis for the development of an algorithm for monitoring patients with generalized seborrheic dermatitis of varying severity.

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Annotation. The purpose of the work is to analyze the scientific literature regarding the constitutional and psychological features of the occurrence and course of seborrheic dermatitis (SD). A review of the literature indicates that diabetes is a multifactorial disease with complex and multifaceted pathogenesis. The works of recent years reflect the involvement in the pathogenesis of SD of virtually all integrating systems of the organism and the main links of its basic functional systems. In the phenotypic manifestation of SD involved both exogenous (physico-chemical, biological) and endogenous (nervous system, genetic predisposition and immune disorders) factors. The pathogenetic mechanisms of dermatitis are polymorphic and do not contradict but complement each other. Studying the constitutional and psychological features of SD in Ukrainian residents is thus a promising area of research.