Lettuce (Lactuca sativa L.) is a leaf vegetable plant from the Compositae (Asteraceae) family that has high economic value. Lettuce contains many minerals and vitamins that are beneficial for health. Lettuce plants have wavy leaves, jagged, fresh green and some are red. There are several challenges in the cultivation of lettuce among them are pest and disease disorders such as snails, nematodes, leaf rot, and root rot. This study aims to determine the type of pathogenic fungi that cause disease and the percentage of disease in Lettuce in Serang Village, Karangreja District, Purbalingga Regency. This study uses a survey method with sampling conducted by purposive random sampling in two different locations. The identification process is done by macroscopic and microscopic observations. The observations were determined by the identification book and continued with the Koch Postulate test. The results of research at the Vegetable Plant Center in Serang Village, Karangreja Subdistrict, Purbalingga Regency, obtained the type of lettuce disease is a leaf rot disease (bottom rot) and leaf spot. Leaf rot is caused by the fungus Rhizoctonia sp. and leaf spot disease caused by the fungus Nigrospora sp. The most common disease in lettuce is leaf rot by Rhizoctonia sp. with a frequency of occurrence as much as 206 times and the percentage of disease by 52.82%.
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