Increasing the amount of feeding in shrimp culture feeding will trigger the accumulation of organic matter and toxic compounds in the form of waste in the pond. One of the effort that can be done is bioremediation or the return system of environmental conditions that are polluted through the addition of certain bacteria. This research aims to identify bioremediation degradation bacteria in shrimp pond waste. This study was held in November 2019 -March 2020. Twelve isolates of bacteria wich isolated from the shrimp pond sediment at Pasir Sakti, East Lampung and cultured on sewage media. Subsequent samples were screened to select total ammonia nitrogen (TAN) degradation bacteria candidates. Bacterial isolates with the best activity are subsequently identified morphologically including cell form, Gram test, motility, and molecular identification with 16S rRNA sequential analysis. The results showed that the best isolate were able to reducing TAN was T4.10 isolate with activity able to reduce TAN by 0,404 mg/L. Morphological, biochemical, and molecular identification confirm that the isolate was 100% Bacillus megaterium bacteria. These bacteria can be used as bioremediation candidates. Shrimp pond waste will be degraded into compounds that can be reused for shrimp metabolic processes, so the sustainability aquaculture can happened.
This study aims to determine the effectiveness of supplementation of sodium (Na) alginate Sargassum sp. from Lampung waters to enhance the non-specific immune response and the resistence of Pacific white shrimp (Litopenaeus vannamei) against white spot disease. This study used a completely randomized design with 3 treatments, namely feeding without alginate supplementation (A) or control, dietary Na alginate Sargassum supplementation at a dose of 2.0 (B), and 4.0 g kg−1 feed (C), each with four replications, for 14 days. Hemolymph sampling was performed on days 0, 7 and 14 to observe the parameter of shrimp hematology included total haemocyte count (THC), phagocytocyte activity (PA), phagocytic index (PI), and total plasma protein (TPP). The hepatopancreas histology profile and water quality were observed at the end of treatment. Data were analyzed by Anova at 95% of confidence interval and continued with Duncan test. Result showedthat the supplementation of Na alginat from Sargassum sp. significantly enhance several shrimp immune response namely THC, PA, and TPP. Meanwhile, the histology of the hepatopancreas of both shrimp treatment and control showed not significantly effect on tissue damage. The water quality was still in normal condition during the treatment. The results of this study indicate that the application of Sargassum alginate supplementation at a dose of 2gr/kg of feed is the best treatment to enhance the immune response of Pacific white shrimp.
Amylolytic and proteolitic activity from thermophilic bacteria have been observed from the Way Panas hot springs, Kalianda, South Lampung. This study aims to isolate and screen amylase and protease enzyme-producing thermophilic bacteria and their potential in fisheries. A total of 8 bacterial isolates were examined from the Way Panas hot spring, Kalianda. Each was cultured on selective medium for amylase and protease production. The results showed that 4 bacterial isolates were able to hydrolyze starch (A.WP.50.1, A.WP.50.1, A.WP.50.3, and A.WP.50.4) were able hydrolyze starch and no bacterial isolates had hydrolysis activity against protein. The highest activity of amylolitic was achieved by A.WP.50.4 bacteria isolate. Based on the phenotype, biochemistry assay, and 16s rDNA sequencing, the A.WP.50.4 bacteria isolate have high similarity and identified as Bacillus cereusAmylolytic and proteolitic activity from thermophilic bacteria have been observed from the Way Panas hot springs, Kalianda, South Lampung. This study aims to isolate and screen amylase and protease enzyme-producing thermophilic bacteria and their potential in fisheries. A total of 8 bacterial isolates were examined from the Way Panas hot spring, Kalianda. Each was cultured on selective medium for amylase and protease production. The results showed that 4 bacterial isolates were able to hydrolyze starch (A.WP.50.1, A.WP.50.1, A.WP.50.3, and A.WP.50.4) were able hydrolyze starch and no bacterial isolates had hydrolysis activity against protein. The highest activity of amylolitic was achieved by A.WP.50.4 bacteria isolate. Based on the phenotype, biochemistry assay, and 16s rDNA sequencing, the A.WP.50.4 bacteria isolate have high similarity and identified as Bacillus cereus
Awareness of Mud Crab over exploitation in Mangkang Wetan Waters has been noticed. One of the reference information is the growth study to determine the condition of the mud crab population. High demand encourages the fisherman to catch more, which leads to overexploitation in nature. The study aimed to estimate the growth, mortality, and exploitation rate of mud crabs. The 921 mud crabs samples were collected from Mangkang Wetan Waters from October 2018 to January 2019. The method used was the survey method. The crabs were taken once a week for 4 months. The width and weight of crab carapace were measured. The growth rate of S. serrata was 0.93/year (male) and 0.69/year (female). The natural mortality rate of S. serrata was 1.08/year (male) and 0.89/year (female), the mortality of catch (F) was 0.55/year (male) and 1.09/year (female). The rate of exploitation of male S. serrata reached 34%, and the rate of exploitation of female S. serrata was 55%. The exploitation of female S. serrata shows that overexploitation has occurred because the optimum value of exploitation (E-OPTIMUM) is equivalent to E=50%.
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