No abstract

Medicinal plants are widely used in folk medicine but quite often their composition and biological effects are hardly known. Our study aimed to analyze the composition, cytotoxicity, antimicrobial, antioxidant activity and cellular migration effects of Anthyllis vulneraria, Fuchsia magellanica, Fuchsia triphylla and Lysimachia nummularia used in the Romanian ethnomedicine for wounds. Liquid chromatography with mass spectrometry (LC-MS/MS) was used to analyze 50% (v/v) ethanolic and aqueous extracts of the plants’ leaves. Antimicrobial activities were estimated with a standard microdilution method. The antioxidant properties were evaluated by validated chemical cell-free and biological cell-based assays. Cytotoxic effects were performed on mouse fibroblasts and human keratinocytes with a plate reader-based method assessing intracellular adenosine triphosphate (ATP), nucleic acid and protein contents and also by a flow cytometer-based assay detecting apoptotic–necrotic cell populations. Cell migration to cover cell-free areas was visualized by time-lapse phase-contrast microscopy using standard culture inserts. Fuchsia species showed the strongest cytotoxicity and the highest antioxidant and antimicrobial activity. However, their ethanolic extracts facilitated cell migration, most probably due to their various phenolic acid, flavonoid and anthocyanin derivatives. Our data might serve as a basis for further animal experiments to explore the complex action of Fuchsia species in wound healing assays.

The improved HPLC/DAD-ESI/MS method was successfully utilised for the characterisation and quantitation of the phenolic compounds in C. avellana bark and leaves extracts.

A nontargeted, effect-directed screening (bioprofiling) and a subsequent highly targeted characterization of antibacterial compounds from plant matrices is demonstrated on the example of Solidago virgaurea root extracts. The procedure comprises high-performance thin-layer chromatography (HPTLC) coupled with six bacterial bioassays including two plant pathogens, a radical scavenging assay, an acetylcholinesterase assay as well as in situ and ex situ mass spectrometric analyses. In situ mass spectra were directly recorded from the adsorbent using the Direct Analysis in Real Time interface (HPTLC-DART-MS), whereas ex situ mass spectra were recorded using an elution head-based interface (HPTLC-ESI-MS). For further bioassay-guided isolation of the main antimicrobial compounds, flash chromatographic fractionation and semipreparative high-performance liquid chromatographic purification were used and nuclear magnetic resonance data allowed the identification of the unknown antimicrobial compounds as 2Z,8Z- and 2E,8Z-matricaria esters. The discovered antibacterial activity was confirmed and specified by a luminometric assay and as minimal inhibitory concentration in the liquid phase.

Diarylheptanoids are a class of secondary plant metabolites with a wide variety of bioactivity. Research on their phytochemistry and phytoanalysis is rapidly growing and the number of identified structures bearing the aryl-C-aryl skeleton is at present approaching 500. Historically, the yellow pigment curcumin has been characterized as the first diarylheptanoid and the extensive research on naturally occurring analogues is still ongoing. In this review, studies dealing with the characterization of linear and cyclic derivatives are discussed from the phytoanalytical point of view. Isolation, fractionation and purification strategies from natural sources along with their chromatographic behavior and structural characteristics are discussed. The role of various techniques used for the extraction (such as Soxhlet extraction, sonication, maceration/percolation, microwave-assisted extraction, supercritical carbon dioxide extraction); isolation (liquid-liquid extraction, column chromatographic techniques, preparative thin-layer and high-performance liquid chromatography, centrifugal partition chromatography, counter-current chromatography); separation (thin-layer chromatography, high-performance liquid chromatography, gas chromatography, capillary electrophoresis) and structural characterization (UV/Vis spectroscopy, infrared spectroscopy, X-ray crystallography, mass spectrometry, nuclear magnetic resonance spectroscopy and circular dichroism spectroscopy) are critically reviewed.