Pani heloch (Antidesma montanum) is traditionally used to treat innumerable diseases and is a source of wild vegetables for the management of different pathological conditions. The present study explored the qualitative phytochemicals; quantitative phenol and flavonoid contents; in vitro antioxidant, anti-inflammatory, and thrombolytic effects; and in vivo antipyretic and analgesic properties of the methanol extract of A. montanum leaves in different experimental models. The extract exhibited secondary metabolites including alkaloids, flavonoids, flavanols, phytosterols, cholesterols, phenols, terpenoids, glycosides, fixed oils, emodines, coumarins, resins, and tannins. Besides, Pani heloch showed strong antioxidant activity (IC50 = 99.00 µg/mL), while a moderate percentage of clot lysis (31.56%) in human blood and significant anti-inflammatory activity (p < 0.001) was achieved with the standard. Moreover, the analgesic and antipyretic properties appeared to trigger a significant response (p < 0.001) relative to in the control group. Besides, an in silico study of carpusin revealed favorable protein-binding affinities. Furthermore, the absorption, distribution, metabolism, excretion, and toxicity analysis and toxicological properties of all isolated compounds adopted Lipinski’s rule of five for drug-like potential and level of toxicity. Our research unveiled that the methanol extract of A. montanum leaves exhibited secondary metabolites that are a good source for managing inflammation, pyrexia, pain, and cellular toxicity. Computational approaches and further studies are required to identify the possible mechanism which responsible for the biological effects.
The methanolic extract of Duabanga grandiflora (Roxb. ex DC.) Walpers leaves was investigated to explore in-vitro anti-arthritic, thrombolytic and antioxidant potential and subsequent detection of preliminary phytochemicals. It is used for treating various diseases by folk practitioners and rural people. Initially, BSA protein denaturation method was employed to evaluate in-vitro anti-arthritic potency, where the extract exhibited potent anti-arthritic activity with 73.52±1.01% inhibition of BSA denaturation at a dose of 1000 μg/ml compared to the standard dichlofenac sodium (83.66±1.03% inhibition at 1000 μg/ml dose). In-vitro thrombolytic efficacy was determined using streptokinase (positive control) and water (negative control), and the average lysis of blood clot was found to be 31.12±1.09%, which is indicative towards the thrombolytic effect of the extract. Antioxidant activity was evaluated by the DPPH radical scavenging method followed by the total phenolic and flavonoid content determination. The crude methanolic extract revealed the IC50 value of 129.31μg/ml and 204.89μg/ml against DPPH and standard ascorbic acid, respectively. The total phenolic content and total flavonoid content of the plant leaves were observed to be 52.81±1.74μg/ml and 25.77±1.01μg/ml, respectively. The results indicate that the leaves of D. grandiflora possess favorable anti-arthritic activity, moderate thrombolytic and excellent antioxidant activity, which is demonstrating the presence of alkaloids, phenols, flavonoids and many other biologically important phyto-constituents that needs further exploration. Bangladesh Pharmaceutical Journal 23(1): 54-60, 2020
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