Fermented rice bran (FRB) is known to protect mice intestines against dextran sodium sulfate (DSS)-induced inflammation; however, the restoration of post-colitis intestinal homeostasis using FRB supplementation is currently undocumented. In this study, we observed the effects of dietary FRB supplementation on intestinal restoration and the development of fibrosis after DSS-induced colitis. DSS (1.5%) was introduced in the drinking water of mice for 5 days. Eight mice were sacrificed immediately after the DSS treatment ended. The remaining mice were divided into three groups, comprising the following diets: control, 10% rice bran (RB), and 10% FRB-supplemented. Diet treatment was continued for 2 weeks, after which half the population of mice from each group was sacrificed. The experiment was continued for another 3 weeks before the remaining mice were sacrificed. FRB supplementation could reduce the general observation of colitis and production of intestinal pro-inflammatory cytokines. FRB also increased intestinal mRNA levels of anti-inflammatory cytokine, tight junction, and anti-microbial proteins. Furthermore, FRB supplementation suppressed markers of intestinal fibrosis. This effect might have been achieved via the canonical Smad2/3 activation and the non-canonical pathway of Tgf-β activity. These results suggest that FRB may be an alternative therapeutic agent against inflammation-induced intestinal fibrosis.
Fermented rice bran (FRB), a prospective supplement, has been proven to ameliorate certain medical conditions. However, its nutraceutical effect on muscle atrophy has never been investigated. The present study aimed to evaluate the effect of FRB on muscle atrophy in a streptozotocin (STZ)-induced diabetic rat model. Three groups of Sprague-Dawley rats, namely the control, STZ, and FRB groups, were treated as follows. The diabetic groups (STZ and FRB) were injected intraperitoneally with STZ (40 mg/kg BW), whereas the control group was injected with the vehicle. The STZ and control groups were fed the AIN93M diet, and the FRB group was fed 10% of FRB based on the AIN93M diet. The diabetic groups had reduced muscle size compared to the control group; however, these changes were alleviated in the FRB group. Moreover, the FRB group had a significantly lower expression of FBXO32/Atrogin-1 and TRIM63/MuRF1 (p < 0.05) due to blocked NF-κB activation. In conclusion, the anti-inflammatory effect of FRB may be beneficial for ameliorating muscle atrophy in diabetic conditions.
BackgroundWe have previously reported that ingestion of adenosine (ADN) and adenosine-5′-monophosphate (AMP) improves abnormal glucose metabolism in the stroke-prone spontaneously hypertensive rat model of non-obesity-associated insulin resistance. In this study, we investigated the effect of ADN and AMP ingestion on glucose metabolism in mice with high-fat diet-induced obesity.MethodsSeven-week-old C57BL/6 J mice were administered distilled water (as a control), 10 mg/L ADN, or 13 mg/L AMP via their drinking water for 14 or 25 weeks, during which they were fed a high-fat diet. Oral glucose tolerance test (OGTT) was conducted on 21-week-old mice fasted for 16 h. Insulin tolerance test (ITT) was performed on 22-week-old mice fasted for 3 h. Blood and muscle were collected for further analysis of serum parameters, gene and protein expression levels, respectively.ResultsGlucose metabolism in the ADN and AMP groups was significantly improved compared with the control. OGTT and ITT showed that ADN and AMP groups lower than control group. Furthermore, phosphorylation of AMP-activated protein kinase (AMPK) and mRNA levels of genes involved in lipid oxidation were enhanced in the skeletal muscle of ADN- and AMP-treated mice.ConclusionThese results indicate that ingestion of ADN or AMP induces activation of AMPK in skeletal muscle and mitigates insulin resistance in mice with high-fat diet-induced diabetes.Electronic supplementary materialThe online version of this article (10.1186/s12906-018-2367-6) contains supplementary material, which is available to authorized users.
ABSTRAKUMKM (Usaha Mikro Kecil dan Menengah) Pangan perlu memiliki SNI produk untuk dapat bersaing di era pasar bebas. Sertifikat SNI Produk dapat diberikan oleh lembaga sertifikasi, dengan syarat, industri tersebut telah mengimplementasikan Sistem Manajemen Mutu SNI ISO 9001. Kegiatan ini bertujuan untuk mengidentifikasi kesiapan industri kecil (pangan) dalam memenuhi klausul SNI ISO 9001 dan mendampingi mereka dalam memperbaiki dokumen yang diperlukan. Kegiatan dilakukan selama 2 bulan dengan sasaran 24 UMKM Pangan di Palu (Sulawesi Tengah) yang dipilih secara purposive. Hasil kegiatan menunjukkan bahwa semua UMKM Pangan belum menerapkan semua aspek CPMB (Cara Produksi Makanan yang Baik). UMKM terbaik hanya memenuhi 21% klausul SNI ISO 9001. Pemenuhan terhadap klausul SNI ISO 9001 meningkat antara 4586% setelah dilakukan pendampingan.Kata kunci: CPMB, SNI ISO 9001, SNI Produk, UMKM Pangan ABSTRACTSmall and Medium Industries of the Food (Food SME's) needs to have SNI products to be competitive in the free market era. SNI certificate product can be provided by the certification body, on the condition that the industry has to implement ISO 9001. This activity aims to identify the readiness of small industries (food) to meet the clauses of ISO 9001 and assist them in improving the necessary documents. Activities carried out for 2 months with 24 samples of small industries (food) in Palu (Central Sulawesi) were selected purposively. The results showed that all the small industries yet to implement all aspects of GMP. Small industry best only meet 21% of the clauses of ISO 9001. Compliance with the clauses of ISO 9001 increased by between 4586% after mentoring Keywords: GMP, ISO 9001, SME's, SNI Product PENDAHULUANUsaha Mikro Kecil Menengah (UMKM) di Indonesia, terutama UMKM Pangan, memiliki keterbatasan dalam menghadapi persaingan di era pasar bebas. Persaingan tidak hanya antar IK Pangan tetapi juga persaingan menghadapi Industri Besar dan industri dari luar negeri dengan dibukanya Masyarakat Ekonomi ASEAN (MEA). Keterbatasan utama UMKM adalah ketidakmampuan dalam memberikan jaminan mutu kepada konsumen untuk produk yang dihasilkannya.Guna memenuhi persyaratan peraturan perdagangan international dan untuk memperkuat posisi perusahaan di persaingan global, maka perusahaan pangan perlu menerapkan sistem jaminan mutu (Karipidis et al. 2009). Sistem jaminan mutu yang berkembang dan umum digunakan dalam industri pangan adalah HACCP, ISO 22000, dan ISO 9001. Seluruh sistem jaminan mutu yang diterapkan di industri pangan memerlukan persyaratan dasar berupa Cara Produksi Makanan yang Baik (CPMB).Persyaratan dasar untuk menghasilkan produk yang bermutu dan aman berupa CPMB
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.