The aim of this research is the detection and analysis of existing trends in the Meta River, Colombia, based on the streamflow records from seven gauging stations in its main course, for the period between June 1983 to July 2019. The Meta River is one of the principal branches of the Orinoco River, and it has a high environmental and economic value for this South American country. The methods employed for the trend detection and quantification were the Mann–Kendall (MK) test, the modified MK (MMK) test, and the Sen’s slope (SS) estimator. Statistically significant trends (at a 95% level of confidence) were detected in more than 30% of the 105 evaluated datasets. The results from the MK test indicate the presence of statistically significant downward trends in the upstream stations and upward trends in the downstream stations, with the latter presenting steep positive slopes. The findings of this study are valuable assets for water resources management and sustainable planning in the Meta River Basin.