Terminal restriction fragment length polymorphism (tRFLP) is a potentially high-throughput method for the analysis of complex microbial communities. Comparison of multiple tRFLP profiles to identify shared and unique components of microbial communities however, is done manually, which is both time consuming and error prone. This paper describes a freely accessible web-based program, T-Align (http://inismor.ucd.ie/~talign/), which addresses this problem. Initially replicate profiles are compared and used to generate a single consensus profile containing only terminal restriction fragments that occur in all replicate profiles. Subsequently consensus profiles representing different communities are compared to produce a list showing whether a terminal restriction fragment (TRF) is present in a particular sample and its relative fluorescence intensity. The use of T-Align thus allows rapid comparison of numerous tRFLP profiles. T-Align is demonstrated by alignment of tRFLP profiles generated from bacterioplankton communities collected from the Irish and Celtic Seas in November 2000. Ubiquitous TRFs and site-specific TRFs were identified using T-Align.
In this forum we highlight innovative thought, design, and research in the area of interaction design and sustainability, illustrating the diversity of approaches across HCI communities. ---Lisa Nathan and Samuel Mann, Editors
Indoor environment quality (IEQ) can negatively affect occupant health and wellbeing. Air quality, as well as thermal, visual and auditory conditions, can determine how comfortable occupants feel within buildings. Some can be measured objectively, but many are assessed by interpreting qualitative responses. Continuous monitoring by passive sensors may be useful to identify links between environmental and physiological changes. Few studies localise measurements to an occupant level perhaps due to many environmental monitoring solutions being large and expensive. Traditional models for occupant comfort analysis often exacerbate this by not differentiating between individual building occupants. This scoping review aims to understand IEQ and explore approaches as to how it is measured with various sensing technologies, identifying trends for monitoring occupant health and wellbeing. Twenty-seven studies were reviewed, and more than 60 state-of-the-art and low-cost IEQ sensors identified. Studies were found to focus on the home or workplace, but not both. This review also found how wearable technology could be used to augment IEQ measurements, creating personalised approaches to health and wellbeing. Opportunities exist to make individuals the primary unit of analysis. Future research should explore holistic personalised approaches to health monitoring in buildings that analyse the individual as they move between environments.
In many parts of the world, mechanical heating and cooling is used to regulate indoor climates, with the aim of maintaining a uniform temperature. Achieving this is energy-intensive, since large indoor spaces must be constantly heated or cooled, and the difference to the outdoor temperature is large. This paper starts from the premise that comfort is not delivered to us by the indoor environment, but is instead something that is pursued as a normal part of daily life, through a variety of means. Based on a detailed study of four university students over several months, we explore how Ubicomp technologies can help create a more sustainable reality where people are more active in pursuing and maintaining their thermal comfort, and environments are less tightly controlled and less energy-intensive, and we outline areas for future research in this domain.
To date, research in sustainable HCI has dealt with ecofeedback, usage and recycling of appliances within the home, and longevity of portable electronics such as mobile phones. However, there seems to be less awareness of the energy and greenhouse emissions impacts of domestic consumer electronics and information technology. Such awareness is needed to inform HCI sustainability researchers on how best to prioritise efforts around digital media and IT. Grounded in inventories, interview and plug energy data from 33 undergraduate student participants, our findings provide the context for assessing approaches to reducing the energy and carbon emissions of media and IT in the home. In the paper, we use the findings to discuss and inform more fruitful directions that sustainable HCI research might take, and we quantify how various strategies might have modified the energy and emissions impacts for our participants.
Healthcare studies are moving toward individualised measurement. There is need to move beyond supervised assessments in the laboratory/clinic. Longitudinal free-living assessment can provide a wealth of information on patient pathology and habitual behaviour, but cost and complexity of equipment have typically been a barrier. Lack of supervised conditions within free-living assessment means there is need to augment these studies with environmental analysis to provide context to individual measurements. This paper reviews low-cost and accessible Internet of Things (IoT) technologies with the aim of informing biomedical engineers of possibilities, workflows and limitations they present. In doing so, we evidence their use within healthcare research through literature and experimentation. As hardware becomes more affordable and feature rich, the cost of data magnifies. This can be limiting for biomedical engineers exploring low-cost solutions as data costs can make IoT approaches unscalable. IoT technologies can be exploited by biomedical engineers, but more research is needed before these technologies can become commonplace for clinicians and healthcare practitioners. It is hoped that the insights provided by this paper will better equip biomedical engineers to lead and monitor multi-disciplinary research investigations.
Purpose Buildings and their use is a complex process from design to occupation. Buildings produce huge volumes of data such as building information modelling (BIM), sensor (e.g. from building management systems), occupant and building maintenance data. These data can be spread across multiple disconnected systems in numerous formats, making their combined analysis difficult. The purpose of this paper is to bring these sources of data together, to provide a more complete account of a building and, consequently, a more comprehensive basis for understanding and managing its performance. Design/methodology/approach Building data from a sample of newly constructed housing units were analysed, several properties were identified for the study and sensors deployed. A sensor agnostic platform for visualising real-time building performance data was developed. Findings Data sources from both sensor data and qualitative questionnaire were analysed and a matrix of elements affecting building performance in areas such as energy use, comfort use, integration with technology was presented. In addition, a prototype sensor visualisation platform was designed to connect in-use performance data to BIM. Originality/value This work presents initial findings from a post occupancy evaluation utilising sensor data. The work attempts to address the issues of BIM in-use scenarios for housing sector. A prototype was developed which can be fully developed and replicated to wider housing projects. The findings can better address how indoor thermal comfort parameters can be used to improve housing stock and even address elements such as machine learning for better buildings.
This study uses an incentive-compatible experimental online supermarket to assess whether prior environmentally-friendly behaviour outside the store, and whether carbon taxes motivate sustainable consumption. Previous research suggests that past decisions may influence current decisions, for example because consumers compensate morally desirable and undesirable acts (e.g. high-carbon food baskets may follow past environmentally-friendly behaviours) over time; while carbon taxes have been promoted as effective tools to reduce the carbon footprint of food baskets, despite limited empirical evidence. After controlling for past consumption, results show that being required to recall past environmentally-friendly behaviour before shopping led consumers to purchase more sustainable food baskets. Carbon taxation also strongly reduces the carbon footprint of food baskets, showing no interaction with the recall of past behaviours.
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