As a precursor to planned arboviral vector incrimination studies, an integrated systematics approach was adopted using morphology and DNA barcoding to examine the Culex fauna present in Turkey. The mitochondrial COI gene (658bp) were sequenced from 185 specimens collected across 11 Turkish provinces, as well as from colony material. Although by morphology only 9 species were recognised, DNA barcoding recovered 13 distinct species including: Cx. (Barraudius) modestus, Cx. (Culex) laticinctus, Cx. (Cux.) mimeticus, Cx. (Cux.) perexiguus, Cx. (Cux.) pipiens, Cx. (Cux.) pipiens form molestus, Cx. (Cux.) quinquefasciatus, Cx. (Cux.) theileri, Cx. (Cux.) torrentium, Cx. (Cux.) tritaeniorhynchus and Cx. (Maillotia) hortensis. The taxon formerly identified as Cx. (Neoculex) territans was shown to comprise two distinct species, neither of which correspond to Cx. territans s.s. These include Cx. (Neo.) impudicus and another uncertain species, which may be Cx. (Neo.) europaeus or Cx. (Neo.) martinii (herein=Cx. (Neo.) sp. 1). Detailed examination of the Pipiens Group revealed Cx. pipiens, Cx. pipiens f. molestus and the widespread presence of the highly efficient West Nile virus vector Cx. quinquefasciatus for the first time. Four new country records are reported, increasing the Culex of Turkey to 15 recognised species and Cx. pipiens f. molestus. A new taxonomic checklist is provided, annotated with respective vector competencies for transmission of arboviruses.
Body size is one of the most significant features of organisms and is correlated with a large number of ecological and physiological variables. Similar to size, biological shape is one of the most conspicuous aspects of an organism's phenotype and provides a link between the genotype and the environment. Body size may change with altitude and also habitat differences associated with altitude may affect the biological shape and some morphological characteristics. Four populations of Aedes vexans Meigen occurring in different ecological subregions at altitudes between 808-1,620 m in the Aras Valley from northeastern Turkey were compared using traditional and geometric morphometrics. When the wing shape differences of populations were analyzed by UPGM, the cluster analyses recognized two main groups of populations. Gödekli (808 m) comprised the first group while Zülfikar (848 m), Sürmeli (944 m), and Cilehane (1,620 m) populations comprised the second group. In the second group, Zülfikar and Cilehane populations showed a similar grouping pattern while Sürmeli appeared as a different group. Centroid sizes were used as measures of overall wing size differences among different regions. Aedes vexans from the Sürmeli region had relatively larger wings. Journal of Vector Ecology 36 (1): 30-41. 2011.
Two formulations, VectoBac 12 AS and VectoBac WDG, of Bacillus thuringiensis israelensis (Bti) and one formulation, VectoLex WDG, of Bacillus sphaericus were tested against Anopheles maculipennis, Culex pipiens, Culex theileri, Aedes caspius, and Aedes dorsalis larvae in drainage canals, a flooded plain, and a drainage well in the Igdir Plain of Arafat Valley, Turkey. VectoBac 12 AS applied at 0.5 and 1 liter/ha to a drainage canal provided complete control of Cx. theileri and 80% to 98% control of An. maculipennis. VectoLex WDG at 400 g/ha provided 100% larval control of Cx. pipiens and Cx. theileri in polluted drainage canals. High larval density of Ae. caspius decreased the efficacy of VectoBac 12 AS and VectoBac WDG in the flooded plain. Residual effects of Bti formulations lasted only a few days in the breeding areas. Residual activity of VectoLex WDG was negatively influenced by water depth, organic pollution, and sunlight and positively influenced by low water velocity and emergent vegetation.
During nine consecutive nights in July 2007 (from 18:15-05:45 h), mosquitoes landing-biting on humans were collected outdoors and indoors at the Turkey-Armenia border. A total of 1005 females were collected consisting of nine species. The dominant species was Aedes dorsalis (Meigen) (47.5% of total catch), followed by Anopheles hyrcanus (Pallas) (22.9%), Culex theileri (Theobald) (9.3%),Ae. vexans (Meigen) (6.6%), Ae. caspius (Pallas) (4.9%),Anopheles maculipennis s.l. (Meigen) (3.1%), Culex territans (Walker) (2.8%), Coquillettidia richiardii (Ficalbi) (1.6%), and Cx. pipiens L. (1.5%). The biting rate outdoors (15.1 mosquitoes/human/h) was greater than indoors (3.4 mosquitoes/human/h). The landing-biting of Ae. dorsalis peaked at dusk (19:15-19:45 h) and dawn (04:15-04:45 h). Ae. vexans activity increased soon after dark (20:15-20:45 h) and reached a peak at dawn (04:15-04:45 h). Maximum biting activity of An. hyrcanus and Cx. theileri occurred during the first sampling interval after dusk (20:15-20:45 h). A large number of An. maculipennis s.l. adults were collected during the second half of the night. We believe that these findings will contribute to decisions on the timing of mosquito control in Ararat Valley.
During nine consecutive nights in July 2007 (from 18:15-05:45 h), mosquitoes landing-biting on humans were collected outdoors and indoors at the Turkey-Armenia border. A total of 1005 females were collected consisting of nine species. The dominant species was Aedes dorsalis (Meigen) (47.5% of total catch), followed by Anopheles hyrcanus (Pallas) (22.9%), Culex theileri (Theobald) (9.3%),Ae. vexans (Meigen) (6.6%), Ae. caspius (Pallas) (4.9%),Anopheles maculipennis s.l. (Meigen) (3.1%), Culex territans (Walker) (2.8%), Coquillettidia richiardii (Ficalbi) (1.6%), and Cx. pipiens L. (1.5%). The biting rate outdoors (15.1 mosquitoes/human/h) was greater than indoors (3.4 mosquitoes/human/h). The landing-biting of Ae. dorsalis peaked at dusk (19:15-19:45 h) and dawn (04:15-04:45 h). Ae. vexans activity increased soon after dark (20:15-20:45 h) and reached a peak at dawn (04:15-04:45 h). Maximum biting activity of An. hyrcanus and Cx. theileri occurred during the first sampling interval after dusk (20:15-20:45 h). A large number of An. maculipennis s.l. adults were collected during the second half of the night. We believe that these findings will contribute to decisions on the timing of mosquito control in Ararat Valley.
In this study, carbon nanotube (CNT) supported Pd/CNT catalysts at varying Pd molar ratios (Pd involving among 0.1-20 wt %) are prepared via NaBH4 reduction method. The surface of catalysts prepared for hydrazine electrooxidation are successfully characterized via N2 adsorption-desorption measurements, X-ray diffractometer (XRD), X-ray photoelectron spectroscopy (XPS), and transmission electron microscope (TEM). Electrochemical measurements are performed using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques. According to the characterization results, for 5% Pd/CNT catalyst, the average particle size and the surface area are determined as 5.17 nm and 773.10 m 2 g -1 , respectively. Among Pd containing (0.1-20 wt %) CNT supported catalysts, 5%Pd/CNT catalyst exhibits the best current density as 6.81 mA cm -2 (1122.63 mA mg -1 Pd). Furthermore, 5% Pd/CNT catalyst shows the best charge transfer resistance (Rct) compared to Pd/CNT catalysts. Pd/CNT catalysts are promising anode catalysts for direct hydrazine fuel cells.
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