Long-chain fatty acids have been shown to suppress appetite and reduce energy intake (EI) by stimulating the release of gastrointestinal hormones such as cholecystokinin (CCK). The effect of NEFA acyl chain length on these parameters is not comprehensively understood. An in vitro screen tested the capacity of individual NEFA (C12 to C22) to trigger CCK release. There was a gradient in CCK release with increasing chain length. DHA (C22) stimulated significantly (P , 0·01) more CCK release than all other NEFA tested. Subsequently, we conducted a randomised, controlled, crossover intervention study using healthy males (n 18). The effects of no treatment (NT) and oral doses of emulsified DHA-rich (DHA) and oleic acid (OA)-rich oils were compared using 24 h EI as the primary endpoint. Participants reported significantly (P ¼ 0·039) lower total daily EI (29 % reduction) with DHA compared to NT. There were no differences between DHA compared to OA and OA compared to NT. There was no between-treatment difference in the time to, or EI of, the first post-intervention eating occasion. It is concluded that NEFA stimulate CCK release in a chain length-dependent manner up to C22. These effects may be extended to the in vivo setting, as a DHA-based emulsion significantly reduced short-term EI.
Long chain fatty acids may suppress appetite and reduce energy intake by stimulating release of gastrointestinal hormones such as cholecystokinin (CCK) (1) . Their differential effect on CCK release, energy intake, body weight and body composition is not comprehensively understood (2)(3)(4)(5) . An in vitro screen using STC-1 cells was used to assess the effects of NEFA on CCK production. CCK concentrations were determined by ELISA. The order of fatty acid induced CCK release per mg cellular protein was docosahexaenoic (3.08 ng/mg), eicosapentaenoic (2.26 ng/mg), pinolenic (1.61 ng/mg), linoleic (1.44 ng/mg), oleic (1.39 ng/mg), conjugated linoleic (1.32 ng/mg), lauric (1.17 ng/mg) and ethanol (1.16 ng/mg). DHA stimulated significantly more CCK production than the other NEFA tested (P < 0.01). An acute randomised, controlled, crossover intervention using healthy males (n 18) compared the effect of consuming an emulsified preload containing DHA, oleic acid (OA) or no-treatment on food intake over a 24 h period. Subjects consumed 12 097 kJ after no treatment, 9912 kJ after OA and 8539 kJ after DHA. There was significantly lower energy intake following DHA relative to no treatment (P = 0.039), which was not matched by OA. In a double-blind, randomised, controlled study of the DHA and OA containing emulsions, overweight female (n 40) subjects consumed twice 6 ml daily dose of either emulsions for 12 weeks. At the end of the intervention, mean body weight (kg) was reduced by 3.5 % in the DHA group and by 1.1% in the OA group. BMI (kg/m 2 ) was reduced by 3.9 % in the DHA group and by 1.9% in the OA group. Mean body weight and BMI were significantly reduced for the DHA group compared with the OA group (P = 0.037 and 0.032, respectively).DHA is a stimulant of CCK in STC-1 cells. In two distinct human feeding studies, DHA consumption significantly reduced total daily energy intake and reduced body weight and BMI.
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