Wounds represent a medical problem that contributes importantly to patient morbidity and to healthcare costs in several pathologies. In Hidalgo, Mexico, the Bacopa procumbens plant has been traditionally used for wound-healing care for several generations; in vitro and in vivo experiments were designed to evaluate the effects of bioactive compounds obtained from a B. procumbens aqueous fraction and to determine the key pathways involved in wound regeneration. Bioactive compounds were characterized by HPLC/QTOF-MS, and proliferation, migration, adhesion, and differentiation studies were conducted on NIH/3T3 fibroblasts. Polyphenolic compounds from Bacopa procumbens (PB) regulated proliferation and cell adhesion; enhanced migration, reducing the artificial scratch area; and modulated cell differentiation. PB compounds were included in a hydrogel for topical administration in a rat excision wound model. Histological, histochemical, and mechanical analyses showed that PB treatment accelerates wound closure in at least 48 h and reduces inflammation, increasing cell proliferation and deposition and organization of collagen at earlier times. These changes resulted in the formation of a scar with better tensile properties. Immunohistochemistry and RT-PCR molecular analyses demonstrated that treatment induces (i) overexpression of transforming growth factor beta (TGF-β) and (ii) the phosphorylation of Smad2/3 and ERK1/2, suggesting the central role of some PB compounds to enhance wound healing, modulating TGF-β activation.
Objetivo: Identificar el conocimiento sobre el uso tradicional de Bacopa procumbens que conservan los pobladores del municipio de Huasca de Ocampo, Hidalgo, México. Metodología: El estudio se basó en una entrevista estructurada aplicada a 26 informantes, mediante un muestreo por conveniencia. Se realizó el análisis descriptivo de la información por medio de frecuencias y un análisis multivariado de correspondencias múltiples. Resultados: El conocimiento tradicional de esta planta se concentra en las amas de casa con edad entre 40 y 50 años; este conocimiento fue trasmitido por familiares, el uso tradicional que le dan a la especie B. procumbens es medicinal y se usa para disminuir o aliviar el dolor de estómago, sanar heridas, calmar el dolor de muelas, calmar el dolor de riñón, eliminar la hinchazón y fiebre. La planta es recolectada en el campo, solo en la cantidad que será usada, lo que puede ayudar en parte a la conservación del recurso, aunque debe prevenirse la sobre explotación. Limitaciones: Existen pocas personas que conocen la planta y sus propiedades. Conclusiones: El conocimiento tradicional de la planta se centra en mujeres amas de casa, la forma más común de uso es hervida y se aplica principalmente para dolor de estómago, y otros problemas de salud.
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