<span class="fontstyle0">Artificial insemination (AI) in cattle using Ongole Cross Breed liquid semen with cauda epidydymal plasma-2 (CEP-2) diluent+0.6% Bovine Serum Albumin (BSA) aimed to improve the genetic quality of livestock with high sperm motility on beef cows. Addition of BSA as extracellular cryoprotectant to support egg yolk function to prevent membrane damage caused by cold shock during cold storage. This research was conducted in Tumpang, Pakis, and Singosari Sub-district, Malang Regency using 38 cows that inseminated using frozen semen of Limousine and Simmental breeds as control (19 cows) and liquid semen with CEP-2 + 0.6% BSA as a treatment (19 cows). The reproductive parameters were Non Return Rate (NRR), Service per Conception (S/C) and Conception Rate (CR). Data were analyzed descriptively and continued by chi-square test. The results showed that insemination using frozen and liquid semen did not affect the success rate of AI in beef cows. The NRR</span><span class="fontstyle0" style="font-size: 7pt;">43-63</span><span class="fontstyle0"> value of frozen was 89.40% and liquid semen was 78.94%; the CR value of frozen semen was 84.21% and liquid semen was 57.89%; and the S/C value of frozen semen was 1.21 and liquid semen was 1.42. The conclusions of the study were the values of NRR</span><span class="fontstyle0" style="font-size: 7pt;">43-63</span><span class="fontstyle0">, CR and S/C of frozen higher than liquid<br />semen.</span>
Improving the productivity and genetic quality of local cattle, especially Ongole Cross Bred (known as PO) is applied through Artificial Insemination (AI). The success of AI must be supported by semen quality. Semen processing at 5°C can cause sperm membrane damage resulting in decreased semen quality, especially the viability of sperm. This results in reduced sperm fertility at the time of fertilization with the ovum. Viability is an important indicator for cold semen processing. The use of semen diluents, Cauda Epididymal Plasma-2 (CEP-2), has been shown to be able to maintain semen quality during storage at 5°C. The addition of BSA as an extracellular cryoprotectant supported the function of egg yolk in CEP-2 to prevent cold shock during storage. This study used fresh ejaculate of PO bull with a minimum requirement of 80% viability. The ejaculate was diluted by CEP-2 with BSA level 0; 0.2; 0.4; 0.6; 0.8 and 1%. The diluted ejaculate was stored for eight days at 5°C. The results showed that the viability of sperm fluctuated in line with the length of storage at each level of BSA. Significant influence of BSA on sperm viability occurred at storage day 4 (83.29±3.53)% at 1% level and day 8 (89.36±2.65)% at 0.2 % level (P <0.01). The conclusion was the BSA 0.2% level maintains the highest viability of sperm during eight days of storage. Suggestion for further research was the need for research on motility and abnormality as a support for the successful processing of liquid semen.
This study aimed to determine the accuracy of various thawing duration and temperatures to the quality of Simmental bull frozen semen. The experimental design used was a randomized group design with five treatments and ten replications, then analyzed using Chi-Square to compare the expected percentage of motility is 40%, and total motile spermatozoa are 10 million/straw value of SNI. A further test is carried out with Duncan's Multiple Range Test (DMRT) if there is a difference. The thawing treatments were 30 seconds at 37˚C water temperatures (T0) according to SNI and at 15 seconds (T1), 30 seconds (T2), 45 seconds (T3), and 60 seconds (T4) with 28˚C tap water temperatures. Variables observed involve motility, viability, abnormalities, concentration, and total motile spermatozoa. The data were analyzed using Analysis of Variance (ANOVA) with a significant difference in variance (P<0,05). The results showed that the different duration and temperatures of thawing influence motility (P<0,05) and the total motile spermatozoa, while the viability, abnormalities, and concentration had no influence (P>0,05). The motility and the total motile spermatozoa reach the highest results at the tap water temperatures T3 (P<0,05), while the lowest results were in T1 (P<0,05). It can be concluded that the different duration and temperatures of thawing influence motility and total motile spermatozoa. The best quality of Simmental bull frozen semen can be obtained with thawing using tap water at 28℃ temperatures for 45 seconds.
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