Nucleic acids (DNA) and neurotransmitters integrate together
in
the brain of organisms to make powerful information processing systems.
Dopamine is an important neurotransmitter whose complexation with
DNA in its protonated form has been implemented in several applications
such as sensors, antitumor drugs, and bioengineering. However, the
molecular level understanding about the binding of protonated dopamine
with the different regions of DNA and its effect on the structure
as well as stability of DNA is very limited. The nature of binding
of protonated dopamine with DNA and its effect on the stability and
structural integrity of DNA have been extensively investigated using
different spectroscopic and molecular dynamics (MD) simulation techniques.
Spectroscopic studies suggest that the multimodal interaction of protonated
dopamine with DNA bases in its groove region enhances its stabilization
without causing any perturbation in the canonical form of DNA. MD
simulation study depicts that protonated dopamine intrudes into the
groove region of DNA by replacing the surrounding water and interacts
with the bases in the major and minor grooves of DNA in a multimodal
manner. Electrostatic and dispersion interactions contribute to the
stabilization of the interaction of dopamine with the minor groove
bases of DNA, whereas the role of electrostatic interaction is prominent
in the case of the major groove. The binding of dopamine with the
groove region of DNA enhances the hydrogen bond between its Watson–Crick
base pairs, which results in higher stabilization of DNA as compared
to that in buffer condition. This study provides a molecular level
insight about the binding of dopamine with DNA, which can be useful
in diverse areas ranging from medicine to bioengineering.
A series of coordination polymers synthesized from a bis‐pyridyl linker, namely 4,4′‐azopyridine (L), selected non‐steroidal‐anti‐inflammatory drugs (NSAIDs), namely diclofenac (Dic), ibuprofen (Ibu), flurbiprofen (Flu), mefenamic acid (Mefe), and naproxen (Nap), and Zn(NO3)2 were characterized by single crystal X‐ray diffraction. One of the coordination polymers, namely CP3 derived from Flu, was able to form metallovesicles in DMSO, DMSO/H2O and DMSO/DMEM (biological media) as revealed by TEM, AFM and DLS. Metallovesicle formation by CP3 was further supported by loading a fluorescent dye, namely calcein, as well as an anti‐cancer drug, doxorubicin hydrochloride (DOX), as revealed by UV‐vis and emission spectra, and fluorescence microscopy. DOX‐loaded metallovesicles of CP3 (DOX@CP3‐vesicle) could be delivered in vitro to a highly aggressive human breast cancer cell line, namely MDA‐MB‐231, as revealed by MTT and cell migration assays, and also cell imaging performed under laser scanning confocal microscope (LSCM). Thus, a proof of concept for developing a multi‐drug delivery system derived from a metallovesicle for delivering an anti‐cancer drug to cancer cells is demonstrated for the first time.
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