Nature has offered us diverse curative herbs having with powerful antioxidant phytochemicals. Ocimum (Lamiaceae) is a notable source of volatile oils and flavouring agents in general and primarily of eugenol, methyl eugenol, linalool, methyl chavicol, etc. Karpoora Thulasi is a member of this genus; nevertheless, not much literature has been reported on its safety and antioxidant potential. In this investigation, we did a pre-clinical safety assessment of concentrate of O. kilimandscharicum on Sprague Dawley rodents. Toxicological concordat of the O. kilimandscharicum concentrate was carried out following OECD guidelines 423. Further, to verify the traditional efficacy and elucidate the mechanism, the present study compared in-vitro antioxidant activity of the plant by DPPH, ABTS and Hydroxyl radical scavenging method using ascorbic acid as the standard. In acute oral toxicity, no treatment-related death or toxic signs were observed. Moreover, the study revealed that the O. kilimandscharicum extract could be well tolerated up to the dose 2000 mg/kg body weight and could be classified as Category 5. Moreover, ABTS free radical activity of the extract was 79.2148 while that of DDPH Inhibition potential was found to be 70.72758. Our findings present substantiation that the crude extracts of O. kilimandscharicum is a likely source of natural antioxidants, and this justified its long-established uses. Keywords: Ocimum kilimandscharicum, Acute Toxicity, In-vitro Antioxidant Activity DPPH, ABTS
Pre-ADMET software derived information has significant value in the design of newer potent drug molecules. All these predicted data help us to screen the potent and safe compounds for further their synthesis with its biological evaluation. Benzothiazole is versatile heterocyclic rings associated with multiple biological activities that result to inspire continue developing of Benzothiazole analogues. The antidiabetic activity can be evaluated by inducing the diabetic conditions in the experimental model using streptozotocin (STZ). The present research work was focused on screening of potent Benzothiazole derivatives with the help of Pre ADMET toxicity profile and further evaluating their biological activity in the streptozotocin induced diabetes rat model. Among all the selected compounds 6e and 6f were found more potent for anti-diabetic activity at 350 mg/kg (p. o.).
Draceana cinnabari (Dracaenaceae) is traditionally used in the treatment of wounds, leucorrhea, fractures, and diarrhoea. Herein, we performed a pre-clinical safety evaluation of extract of Draceana cinnabari resin on Spradgue Dawley rats. Toxicological codicil of the extract was carried out following OECD guidelines 423 and 407, with minor changes. DC resin methanol extract administered to the rats by oral gavage at 50, 500, 1500, and 2500 mg/kg body weight daily up to 28 days to male and female rats. Herbal extract could most likely be very much endured up to the dose 2000 mg/kg body weight and could be named Category 4. Oral toxicity studies confirm that Draceana cinnabari has no therapy-related demise or toxic signs on rats. Therefore, the resin, ought to be suitably considered for additional research for its medicinal and therapeutic efficacy. Keywords: Dracaenaceae, DC extract, Resin, Acute toxicity, Sub Chronic Toxicity
Objective: The objective of the study was to investigate the pharmacological evaluation of previously isolated compounds (CR-1 to CR-5) from the areal part of Cuscuta reflexa Roxb. is reported.Methods: The antimicrobial and antioxidant activity of the isolated compounds (CR-1 to CR-5) from C. reflexa was determined by the disc-diffusion method and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) model, respectively. The antimicrobial activity was performed against four strains Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa.Results: The results revealed that highest zone of inhibition is measured by compound CR-5 against E. coli. The antioxidant activity is evaluated for in vitro antioxidant activity using DPPH radical scavenging activity, inhibitory concentration 50% (IC50) (120.92–76.38 %), respectively. The results indicate that isolated compound CR-1 and CR-2 having IC50 76.38 and 76.94 μg/ml, respectively, showed potent antioxidant activity comparable to standard ascorbic acid (IC50 43.42 μg/ml).Conclusion: This study suggests that areal part of C. reflexa have bioactive compounds for a new antimicrobial and antioxidant drug development.
The newly synthesized compounds are being tested for in vitro anticancer activity. The method used for In Vitro testing is Sulforhodamine B assay also known SRB assay. Cell lines were prepared and homogenized and disassociated with the help of trypsin. Then trypsin was inactivated with fetal bovine serum. Then cell concentration was determined. Synthesized molecules were prepared into four different dilutions and exposed to cell lines. The procedure was also compared with standard drug doxorubicin. All the cell medium were incubated 37 degrees centigrade in a humidified incubator with 5 percentage CO. The plates were stained and fixed with trichloroacetic acid. Finally, the plates were incubated in orbital shaker incubator and absorbance was measured in a microplate reader at 510nm. All compounds (1-30) showed the similar anticancer activity of compounds (IA, IB, ID, IE, IF, IIB, IIC, IIIA, IVB, IVF, VA, VC, VD, VE.) were more potent when compared to the rest of the compounds synthesized.
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