Evaluation of preliminary phytochemical screening, acute toxicity and antioxidant profile of Ocimum kilimandscharicum

Abstract

Nature has offered us diverse curative herbs having with powerful antioxidant phytochemicals. Ocimum (Lamiaceae) is a notable source of volatile oils and flavouring agents in general and primarily of eugenol, methyl eugenol, linalool, methyl chavicol, etc. Karpoora Thulasi is a member of this genus; nevertheless, not much literature has been reported on its safety and antioxidant potential. In this investigation, we did a pre-clinical safety assessment of concentrate of O. kilimandscharicum on Sprague Dawley rodents. Toxicological concordat of the O. kilimandscharicum concentrate was carried out following OECD guidelines 423. Further, to verify the traditional efficacy and elucidate the mechanism, the present study compared in-vitro antioxidant activity of the plant by DPPH, ABTS and Hydroxyl radical scavenging method using ascorbic acid as the standard. In acute oral toxicity, no treatment-related death or toxic signs were observed. Moreover, the study revealed that the O. kilimandscharicum extract could be well tolerated up to the dose 2000 mg/kg body weight and could be classified as Category 5. Moreover, ABTS free radical activity of the extract was 79.2148 while that of DDPH Inhibition potential was found to be 70.72758. Our findings present substantiation that the crude extracts of O. kilimandscharicum is a likely source of natural antioxidants, and this justified its long-established uses.
 Keywords: Ocimum kilimandscharicum, Acute Toxicity, In-vitro Antioxidant Activity DPPH, ABTS

Silver nanoparticle biosynthesis utilizingOcimum kilimandscharicumleaf extract and assessment of its antibacterial activity against certain chosen bacteria

“…Plants are rich in secondary metabolites which usually facilitate the synthesis of silver nanoparticles. In a research study conducted by Saxena et al [33], flavonoids, alkaloids, glycosides, saponins, and tannins were present in methanolic extract of O. kilimandscharicum .…”

Acute and 28‐Day Repeated‐Dose Oral Toxicity Assessment of Abelmoschus moschatus Medik. in Healthy Wistar Rats

“…Antioxidants in medicinal plants play a major role in the defense against oxidative damage caused by reactive oxygen species [7,52]. erefore, the total antioxidant activity of the four selected extracts of A. moschatus was assessed by DPPH radical scavenging assay.…”

“…erefore, the total antioxidant activity of the four selected extracts of A. moschatus was assessed by DPPH radical scavenging assay. It is a simple, rapid, and relatively inexpensive assay, widely used in the evaluation of the antioxidant potential of aqueous or organic extracts with hydrophilic and lipophilic compounds [7,53]. e total antioxidant activity is expressed in terms of IC 50 ( Table 5).…”

“…Plants may produce toxic secondary metabolites as natural defenses against adverse conditions [1]. ese specialized secondary metabolites serve as signaling hormones, providing resistance against pests and diseases, attracting pollinators, and defending against herbivorous insects and pathogenic microorganisms [1,[7][8][9]. e secondary metabolites in plants have documented for their potency to modulate and modify biological activities in humans [10].…”

A Comprehensive Review of the Pharmacological Activities of Ocimum kilimandscharicum: Camphor Basil

“…Vero cell lines show that the tissue culture toxicity dose is 500 µg mL −1 for the methanol and aqueous extract of this plant. [ 27 ] Leaf extracts obtained using methanol have undergone preclinical safety assessment in Sprague Dawley pathogen‐free rats and this showed that the rats can tolerate up to 2500 mg kg −1 of the extract without any significant adverse effects like tremors, coma, and fatality. [ 28 ] Essential oils present in the leaves have been hydrodistilled and this is found to inhibit the proliferation of tumor cells with a GI50 of 31.90 mg mL −1 .…”