Summary
The present work aimed to determine the bioactive compounds in two rosemary water extracts (RWE1 and RWE2) and to assess their antimicrobial, anti‐adhesive and antibiofilm potentials against the food‐related Bacillus and Pseudomonas species at concentrations; 4, 8, 12, 16 and 20 mg mL−1. Phenolic compounds and isoflavones in the RWEs were determined using HPLC. The concentrations of most bioactive compounds of RWE1 (benzoic, ellagic, gallic and rosmarinic acids, daidzein and genistein) were higher than that of RWE2. The MIC90 of RWE1 and RWE2 against all tested bacteria was 12 and 16 mg mL−1, respectively. The anti‐adhesive and antibiofilm doses were higher than MIC90. RWE1 and RWE2 showed potential reduction in the bacterial cell adhesion to HEp‐2 cells – 17.5–64.7 and 12.2–52.9%, respectively. In conclusion, this study emphasises the effective use of RWE as a natural anti‐adhesive and antibiofilm agent against Bacillus and Pseudomonas, without difficult extraction procedure.
In this research, some plant seeds powder was evaluated to find their potential effect to rule diseases of food poisoning. Antimicrobial effect of five plant seeds was examined contra Bacillus cereus, Staphylococcus aureus, Escherichia coli, Klebsiella. pneumonia and Candida albicans by using well diffusion method. Antimicrobial activity studies revealed high potential activity of plant seeds powder of Nigella sativa L., cucurbita pepo, Sesamum radiatum, Trigonella foenum-graecum, Linum usitatissimum with variable efficiency contra tested microbial strains with concentration of 100 mg/ml, except Sesamum radiatum scored no effect. The T. foenum and N. sativa seed powder showed the largest inhibition zone (24-20 mm) contra K. pneumonia, followed by S. aureus (20-18 mm) and C. albicans (15mm) respectively. The five plant seeds powder exhibited bacteriostatic and bactericidal effects with MIC’s 20 and MBC 40 mg/ml against K. pneumonia, and MIC’s 40 and MBC 60 mg/ml against S. aureus. The results of this study indicated that plants seeds powder have promising antimicrobial activities and their potential applications in food process. It could be utilized as a natural medicinal alternative instead of chemical substance.
Nanotechnology has become promise field as a new approach for pest managing in recent years. Laboratory experiments were conducted to evaluate the toxicity of synthesized silver nanoparticles (AgNPs) by using aqueous plant extract (Rosmary, Laura and Cardamom) and their concentrations against main stored grain insect pest (Sitophilus granarius), microbial contents and nutritive value. Wheat grain which were treated with Rosmary 5 showed 100% mortality for adults of S. granarius after 120 h., while the same efficacy of Laura5 and Cardamom 5 recorded after 144 h. LC50 and LC90 values of the synthesized silver nanoparticles were identified against S. granarius indicated that the toxicity of AgNPs varied according to the plant extractio and exposure period.The carbohydrate, fiber, fat, protien and ash content of the treated wheat grains were significantly higher than control. While, the microbial contents were not detected after treatments by most AgNPs concentrations. In conclusion, the synthesis of AgNPs with high amounts from each plant extract and lower concentration from AgNo3 (1 µg/ml) can be used as a valuable tool in pest management programs of S. granarius, reducing microbial content, and increasing the nutrition values, that led to increase shelf life of stored wheat grain. The results showed that these compounds could be used as a source of bioactive compounds safely for ecosystem.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.