Hemoglobin was immobilized in Mg(2)Al-Layered Double Hydroxides (LDH) by coprecipation method at pH 9.0. Interactions between Hb and LDH particles were investigated by X-ray diffraction patterns, FTIR, UV-vis, circular dichroism, and fluorescence spectroscopies. Morphology and porosity of Mg(2)Al-Hb(cop) biohybrid are analyzed from SEM and TEM images and permeability measurement. The direct electron transfer of immobilized Hb was studied by cyclic voltammetry, and the electrocatalytic activity was evaluated at glassy carbon modified with this Mg(2)Al-Hb(cop) biohybrid. Even though the percentage of electroactive Hb was less than 2%, this bioelectrode showed a low detection limit (1.5 x 10(-8) M) and a very high sensitivity (37 A/M cm(2)) for the amperometric detection of H(2)O(2).
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.