Room temperatures ionic liquids are considered as miraculous solvents for biological system. Due to their inimitable properties and large variety of applications, they have been widely used in enzyme catalysis and protein stability and separation. The related information present in the current review is helpful to the researchers working in the field of biotechnology and biochemistry to design or choose an ionic liquid that can serve as a noble and selective solvent for any particular enzymatic reaction, protein preservation and other protein based applications. We have extensively analyzed the methods used for studying the protein-IL interaction which is useful in providing information about structural and conformational dynamics of protein. This can be helpful to develop and understanding about the effect of ionic liquids on stability and activity of proteins. In addition, the affect of physico-chemical properties of ionic liquids, viz. hydrogen bond capacity and hydrophobicity on protein stability are discussed.

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The thermodynamics of mixed micellization of amitriptyline hydrochloride (AMT) with ionic liquid-type imidazolium gemini surfactant ([C 10 -4-C 10 im] Br 2 ), was investigated at different mole fractions and temperatures by surface tension measurements. The deviation of the critical micelle concentration (CMC) from the ideal critical micelle concentration (CMC*), micellar mole fraction (X m 1 ) from ideal micellar mole fraction (X ideal 1 ), the values of interaction parameter (b) and activity coefficients (f i ) (for both mixed micelles and mixed monolayer) explained the non-ideal behavior (i.e., synergistic behavior) of binary mixtures. The excess free energy (DG ex ) for the AMT-[C 10 -4-C 10 im] Br 2 binary mixtures explained the mixed micelles stability in comparison to micelles of [C 10 -4-C 10 im] Br 2 and pure AMT. Interfacial parameters, i.e., Gibbs surface excess (C max ), minimum head group area at air/water interface (A min ), free energy of micellization (DG o m ), and standard Gibbs energy of adsorption (DG ads o ) were also evaluated for the systems. The standard entropy of adsorption (DS ads o ) was found higher than the standard entropy of micellization (DS m o ) at all mole fractions of AMT (a 1 ).

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