The aim of the current study is to evaluate the antiinflammatory, antioxidant and analgesic properties of ethanolic (100 and 200 mg/kg, p.o.) and ethyl acetate extracts (100 and 200 mg/kg, p.o.) of Caralluma europaea. Formalin-induced paw licking test, Acetic Acid induced Writhing Test and Hot-PlateTest were used to assess the analgesic activity. Xylene-induced ear edema test was used to evaluate anti-inflammatory activity of those extracts. In this work, the High-Performance Liquid Chromatography technique (HPLC), allowed us to identify and quantify the main phenolic compounds present in ethanolic and ethyl acetate extracts. In vitro anti-oxidant propriety was evaluated using two methods, the 2,2-Diphenyl-1Picrylhydrazyl (DPPH) radical scavenging method and reducing power methods. The main phenols identified were Catechin (24%) and quercetin (18%) in ethanolic extract, while in the ethyl acetate extract, they were quercetin (36%), Pcoumaric (30%) and 2-hydroxycinnamic (25%). Analysis of our results had shown that Caralluma europaea extracts had exhibited a very potent analgesic activity. Percentage of Pain Inhibition (PPI) in the writhing test, 63.60±4.24% for the Ethanolic Extract (EE) (200 mg/kg, p.o.) and 65.39±3.27% for the Ethyl Acetate Extract (EAE). The PPI of early and late phase in the formalin test were respectively, 41% and 73% for EAE (200 mg/kg; p.o), 28% and 75% for EE. In the hotplate test, latency to the thermal stimuli was increased in a dose dependent manner after the administration of EE and EAE. However, the analgesic potential of EAE seems to be higher than EE. Both EE and EAE presented a significant in vitro redox potential and high antiinflammatory activity. Our results have shown that Caralluma europaea is rich in phenolic compounds and possesses an important antinociceptive, anti-inflammatory and anti-oxidant activity.

The leaves of Salvia officinalis L. have a traditional reputation for the management of pain in Morocco. This study was conducted to investigate the curative effects of Salvia officinalis (SO) and its major constituents Rosmarinic (ROS) and Caffeic acids (CAF) on peripheral neuropathic pain in mice. Chronic constriction injury (CCI) was induced in mice, and neuropathic pain behaviors tests were evaluated by mechanical, chemical, thermal sensation tests and functional recovery of the sciatic nerve at different time intervals, i.e., (day 0, 1, 7, 14, and 21). Ethanolic extract of SO (100 and 200 mg/kg, p.o.), ROS (10 and 20 mg/kg, i.p.), CAF (30 and 40 mg/kg, i.p.), and CLOM (5 mg/kg, i.p., a positive control) was given for 21 days after surgery. Hematological and biochemical parameters were also measured as well as histopathological analysis. CCI produced significant development in mechanical and thermal hyperalgesia, cold allodynia, and rise in the sciatic functional index in mice. Chronic treatments with SO extract, ROS, CAF, and CLOM for 3 weeks significantly increased mechanical sensibility, cold, and thermal withdrawal latency and enhanced functional recovery of the injured nerve. The same treatments remarkably ameliorated hematological parameters and did not alter biochemical levels. The histopathological findings had revealed the protective effect of SO, ROS, and CAF against the CCI-induced damage. Our data support the use of SO in folk medicine to alleviate pain. Their main phenolic constituents could be promising antineuropathic compounds, which may be attributed to their biological activities including anti-inflammatory, antioxidant, and neuroprotective effects. SO leaves may be a good candidate to treat neuropathic pain.

No abstract

Malaria is a mosquito-borne infectious disease caused by obligate intraerythrocytic protozoa of the genus Plasmodium. As one of the most devastating global health issues, malaria is a sensitive disease to weather and climate conditions, in such a way the ongoing trends of increasing temperature and more variable weather could lead to malaria transmission spreading. Spatial and temporal variations in precipitation, temperature, and humidity that are projected to take place under different climate change scenarios will impact the biology and ecology of malaria vectors and subsequently the risk of disease transmission. Here, the authors review how climate and climate change may impact malaria transmission. They contrast ecological and behavioral characteristics of malaria vectors and parasites and how weather, climate, climate change, and socioeconomic factors may have very different impacts on their spatiotemporal occurrence and abundance and the resulting malaria risk.

Malaria is a mosquito-borne infectious disease caused by obligate intraerythrocytic protozoa of the genus Plasmodium. As one of the most devastating global health issues, malaria is a sensitive disease to weather and climate conditions, in such a way the ongoing trends of increasing temperature and more variable weather could lead to malaria transmission spreading. Spatial and temporal variations in precipitation, temperature, and humidity that are projected to take place under different climate change scenarios will impact the biology and ecology of malaria vectors and subsequently the risk of disease transmission. Here, the authors review how climate and climate change may impact malaria transmission. They contrast ecological and behavioral characteristics of malaria vectors and parasites and how weather, climate, climate change, and socioeconomic factors may have very different impacts on their spatiotemporal occurrence and abundance and the resulting malaria risk.