Photoluminescence lifetime microscope spectrometer based on timecorrelated singlephoton counting with an avalanche diode detector Rev. Sci. Instrum. 61, 11 (1990); 10.1063/1.1141885Time response of small sideon photomultiplier tubes in timecorrelated singlephoton counting measurements Rev. Sci. Instrum. 59, 499 (1988);We report the operation of a new type of fluorescence lifetime imaging camera based on the time-correlated single-photon counting ͑TCSPC͒ technique. To the best of our knowledge the application-specific integrated circuit ͑ASIC͒ used in the camera is the first ASIC designed for the field of fluorescence. The ASIC also forms the basis for the first read-out system for single-photon timing array detectors and is capable of multiplexing and routing counts from up to sixteen detection channels, while preserving their timing characteristics with picosecond resolution. In conjunction with an array detector such as a multianode MCP-PM this ASIC allows multiple fluorescence decays to be routinely and simultaneously acquired using a single set of TCSPC timing electronics. To demonstrate one practical application of this technology, we have observed for the first time the spatial distribution of fluorescence lifetime contours through a strongly self-absorbing sample, and the effects observed demonstrate how differences in optical geometry can contribute to the lack of consistency between results obtained in different laboratories.
We review the technique of multiplexed time-correlated single-photon counting whereby multiple fluorescence decay curves are recorded in parallel by statistically time-sharing a single time-to-amplitude converter. Application of the multiplexing technique to measuring the fluorescence lifetime topography of a self-absorbing sample is demonstrated. Further possibilities are discussed for multiplexed optical fiber sensor networks with built-in intelligence for detecting and discriminating between different metal ions in solution.
We report the miniaturization of single-photon timing fluorescence lifetime spectroscopy using picosecond diode laser excitation and liquid light guide collection of different timecorrelated signals in a multiplexed arrangement. Liquid light guide coupling permits multiple channels of fluorescence decay information with respect to wavelength, polarization, sensor, etc, to be collected simultaneously and eliminates a common source of error in convolution analysis. The instrumental performance is demonstrated in a study of perylene fluorescence quenched by cobalt ions in micelles
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